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This study assessed the impact of long-term conservation tillage and N fertilization in wheat-soybean rotation on soil aggregate distribution, nutrients stocks and functions of soil microorganisms. They compared conventional tillage and minimal tillage and 2 N levels; 0 and 200kgN/ha. Residues were incorporated to 25 cm in CT and 50% to 15cm in MT. Tillage was the most discriminant factor explaining 72% and 60% of total variance of soil parameters at 0-15 cm and 15-30 cm soil depth. All enzyme activities were higher under MT, whereas the majority of soil chemical parameters were higher under N200. They conclude that maintaining or even increasing SOC conservation in their silt loam soils may require both reduced tillage systems and N fertilization, shifting microbial community toward taxa more effective in contrasting soil degradation