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The paper suggests that the increase in carbon sequestration under long-term NT and cover crop management is a sustainable agricultural system with an increase in SOC and soil respiration. This study looks at the addition of biochar on soil respiration in Japan. Long-term tillage practices, such as NT and moldboard plowing (MP), cover crops such as rye (RY) and fallow (FA), and biochar application such as with biochar (WB) and no biochar (NB) were applied to a split–split plot in a randomized complete block design with four replications. NT had higher respiration compared to MB. Rye had a higher respiration than fallow. Biochar significantly reduced respiration in the soybean and cover crop growing seasons but enhanced carbon sequestration. It reduced respiration by regulating the soil temperature, soil moisture and protect the SOC from decomposition. Biochar also increased SOC, decreased bulk density that improved soil porosity.