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This article looks at the role of crop rotation on soil aggregation and associated organic carbon (OC). The used 2199 paired observations from 53 studies to elucidate the rotations role in soil aggregation and associated OC and to identify optimal climatic, edaphic and agronomic factors. Overall, rotation improved aggregate and OC contents in all aggregate classes compared to monoculture. Greater increases in soil aggregation and associated OC induced by crop rotation were associated with sub-soiling, no-till, straw retention, combined manure-inorganic fertilizers, and a lower nitrogen fertilization input rate with more rotation cycles and longer rotation length. Climate and initial soil OC were important for the benefits of rotation. They conclude rotation is important for the sustainability of agroecosystems.