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This paper analyzed two 6-year long component omission experiments on two different soil types (sandy and clay) in terms of soil chemistry, residue decomposition and arbuscular mycorrhizal fungi (AMF) colonization of maize roots. Soil chemical properties only differed across systems in the sandy soil mulched systems under no-tillage (NT) resulting in increased soil organic carbon levels, total nitrogen, and soil available phosphorus as compared to conventional tillage with no mulch or rotation (CT). Systems with NT and rotation resulted in highest AM fungal root colonization rate of maize at the clay soil location. Total plant N uptake was almost 2-fold higher in tilled and no-tilled systems with both mulch and rotations. They used structural equation modeling to disentangle the links between cropping systems, soil chemical and biological properties, plant nutrient uptake, and maize grain yield. They conclude that a more holistic approach to cropping system assessment that includes a higher number of abiotic and biotic determinants is needed to evaluate the drivers of yield. .