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This paper reports the results from a 9-year field study on a wheat-maize-soybean rotation in Italy where NT combined with grassy and legume cover crops (CC) were studied in terms of yield, C and N input from the main and cover crops, soil aggregation, and C and N sequestration rates in comparison with conventional tillage (CT). They showed that yield of winter wheat, maize, and soybean were never reduced under both NT treatments, neither during the transition phase, nor afterwards. Rye and hairy vetch provided the same amount of biomass and C input, although vetch doubled N input compared with rye. Both NT plus rye or vetch promoted C and N sequestration mainly due to increase in macroaggregate associated C and N. They conclude that since there was no difference between cover crops in terms of biomass input, and C and N sequestration potential, that cover crop biomass production (rather than biomass quality) and retention onto the soil as residue were the main drivers of soil C and N sequestration.