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This paper looks at the effect of agricultural practices in the maize rhizosphere on soil bacterial communities in Mexico. Maize and bulk rhizosphere soil from conventional (CP) and CA practices were sampled at three growth stages and used 16S rRNAto assess bacterial diversity and community structure. Results showed that CA positively affected soil bacteria compared to CP. The bacterial community in the maize bulk soil resembled each other more than in the rhizosphere of CA and CP. Although agricultural practices did not alter the effect of the rhizosphere on the soil bacterial communities in the flowering and grain filling stage, they did in the vegetative stage.