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This field study evaluated the effects on cropping sequences and tillage systems on SOC and N fractions. No-till (NT) and rotary tillage (RT) were combined with four cropping sequences: wheat-soybean-wheat-maize (WSWM), wheat-maize-wheat-soybean (WMWS), wheat-soybean-wheat-soybean (WS) and wheat-maize-wheat-maize (WM). Tillage systems affected SOC and N in topsoil rather than deeper, but cropping sequences in the whole sampling depth (0-50cm). NT had significantly higher SOC concentrations than RT at the 0–10- (17% higher) and 20–30-cm (19% higher) soil layers. NT had 17% significantly higher N contents than RT at the 0–10-cm soil layer, but RT had 21% significantly higher N accumulation at the 10–20-cm soil layer. The particulate organic carbon (POC) was highest in WM and lowest in WS cropping sequence at 0–10-cm soil depth. Particulate organic nitrogen (PON) was significantly higher in soybean-included cropping sequences only at 0–10-cm soil depth. Overall, their findings suggested that NT especially with soybean could be a suitable practice to sequester SOC and N in the North China Plain.