from Flow Cytometry to Cytomics
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Scooped by Gilbert C FAURE
September 1, 2013 3:43 AM
Scoop.it!

Counting Cells with a Hemocytometer

There can be tens of thousands of cells in one milliliter of culture medium. So how are cells counted?

 

Gilbert C FAURE's insight:

It is how techniques are taught or learned now! Thanks Youtube

 

Obviously, this first Scoop is not significant of the information you will find through the following 187 pages of this topic,

with many recent informations of developments of

flow cytometry,

image cytometry...

focusing particularly on the detection, quantification, characterization of rare events (CTCs

https://www.scoop.it/topic/from-flow-cytometry-to-cytomics?q=ctc

CECS, microvesicles...

 

in peripheral blood and biological fluids in the context of liquid biopsy

https://www.scoop.it/topic/from-flow-cytometry-to-cytomics?q=liquid+biopsy

 

more than 3000 scoops, >5200 visitors, 10700 views (march 2015)

juin 2019 more than 4400 scoops, >13K visitors, >31,6 K views

august 2022, More than 4.7K scoops, more than 15.4K visitors

 

For data from the curator, see the Nancytomique topic.

http://www.scoop.it/t/nancytomique

 

 

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Scooped by Gilbert C FAURE
December 24, 2024 4:33 AM
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https://www.immunology.org/events/flow-cytometry-making-sense-multiparametric-design

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Scooped by Gilbert C FAURE
November 24, 2024 9:00 AM
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ULB HeLSci - Continuing education in Health and Life Science on LinkedIn: #cytométrie #phénotypage #standardisation #flowjo #seformeraveculbhelsci…

ULB HeLSci - Continuing education in Health and Life Science on LinkedIn: #cytométrie #phénotypage #standardisation #flowjo #seformeraveculbhelsci… | from Flow Cytometry to Cytomics | Scoop.it
Explorez nos modules avancés en #cytométrie en flux !

➡️ Boîte à outils avancée pour réussir son expérience de #phénotypage cellulaire
💻 Module en 2 étapes :…
Marcus Jorelle's curator insight, December 16, 2024 9:26 PM
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November 2, 2024 6:35 AM
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Catherine Alix-Panabières on LinkedIn: #australia #liquidbiopsy #ctc #precisionmedicine

Catherine Alix-Panabières on LinkedIn: #australia #liquidbiopsy #ctc #precisionmedicine | from Flow Cytometry to Cytomics | Scoop.it
🩸Liquid Biopsy Milestones since 1869 ...

✨Wonderful experience at the University of Technology Sydney (UTS) in #Australia.

CHU de Montpellier
Recherche et…
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August 14, 2024 4:31 AM
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Sensitive Microfluidic Sensors Detect Pancreatic Cancer Cells

Sensitive Microfluidic Sensors Detect Pancreatic Cancer Cells | from Flow Cytometry to Cytomics | Scoop.it
Researchers developed a microfluidic platform using surface-enhanced Raman spectroscopy (SERS) to detect single pancreatic cancer cells. The platform successfully differentiated between cancer stages, offering a promising tool for early cancer diagnosis through molecular analysis of individual...
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May 14, 2024 2:59 AM
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Oliver Burton sur LinkedIn : The scale of the plots presenting your flow cytometry data are actually…

Oliver Burton sur LinkedIn : The scale of the plots presenting your flow cytometry data are actually… | from Flow Cytometry to Cytomics | Scoop.it
The scale of the plots presenting your flow cytometry data are actually critically important in getting good results with high dimensional data analysis. The…
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May 2, 2024 4:27 AM
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Melanie O'Donahue on LinkedIn: Advances in Cytometry: Applications

Melanie O'Donahue on LinkedIn: Advances in Cytometry: Applications | from Flow Cytometry to Cytomics | Scoop.it
J. Paul Robinson, Pratip Chattopadhyay, & James Jacobberger have completed a new volume in Methods in Cell Biology:

📕Advances in Cytometry:…
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March 11, 2024 10:50 AM
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Panel Design: selecting fluorophores for spectral flow, part 2

Panel Design: selecting fluorophores for spectral flow, part 2 | from Flow Cytometry to Cytomics | Scoop.it
How do we decide which fluorophores to use for spectral flow cytometry? Last week I suggested treating spectral cytometers just like conventional cytometers with lots of detectors. For the purpose of fluorophore selection, this means picking one fluorophore to peak in detector. I'll show some exceptions to this rule, and also show how they aren't as exceptional as they might first appear. The guidelines I'm presenting here are most important as you increase the size and complexity of your panels
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February 13, 2024 11:10 AM
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CBD’s Pobezinsky and Pobezinskaya Use Flow Cytometry to Determine How Tumor Cells Outwit the Body’s Immune System : Institute for Applied Life Sciences : UMass Amherst

CBD’s Pobezinsky and Pobezinskaya Use Flow Cytometry to Determine How Tumor Cells Outwit the Body’s Immune System : Institute for Applied Life Sciences : UMass Amherst | from Flow Cytometry to Cytomics | Scoop.it
The Center for Bioactive Delivery's Pobezinsky and Pobezinskaya have analyzed and described what they call the "mosquito effect," shedding light on how certain pathogens can outwit the body's immune system.
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John Gordon sur LinkedIn : Extracellular vesicles as tools and targets in therapy for diseases -…

John Gordon sur LinkedIn : Extracellular vesicles as tools and targets in therapy for diseases -… | from Flow Cytometry to Cytomics | Scoop.it
#ExtraCellularVesicles | Impressively Comprehensive OPEN ACCESS Review Covering Role for #EVs in #Cancer #NeuroDegenerativeDiseases #Diabetes…
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https://www.linkedin.com/posts/catherine-alix-panabi%C3%A8res-b5969942_cancer-liquidbiopsy-ctc-activity-7153076654972502016-mQVC?utm_source=share&utm_medium=member_desktop

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Frontiers | A comprehensive battery of flow cytometric immunoassays for the in vitro testing of chemical effects in human blood cells

Frontiers | A comprehensive battery of flow cytometric immunoassays for the in vitro testing of chemical effects in human blood cells | from Flow Cytometry to Cytomics | Scoop.it
BackgroundThere is a growing need for immunological assays to test toxic and modulatory effects of chemicals. The assays should be easy to use, reproducible and superior to cell line-based assays. We have therefore developed a comprehensive portfolio of assays based on primary human blood cells that are suitable for testing chemical effects.MethodsThe flow cytometry-based assays were designed to target a wide range of human peripheral blood mononuclear cells and whole blood, including T cells, NK cells, B cells, basophils and innate-like T cells such as γδT, MAIT and NKT cells. We have selected a set of activation markers for each immune cell, e.g: CD154 (T cells), CD137, CD107a (NK cells), CD63 (basophils), CD69, CD83 (B cells), CD69, IFN-γ (MAIT cells) and we selected cell specific stimuli: aCD3 antibodies (T cells); E. coli and cytokines IL-12/15/18 (MAIT cells); CpG ODN2006, R848 or aCD40 antibodies (B cells), fMLP or aFcϵR1 (basophils) or K562 cells (NK cells).ResultsBy selecting immune cell-specific markers and cell-specific stimuli, we were able to induce particular immune responses from the targeted immune cells. For example, the response to stimulation with anti-CD3 antibodies was in 36.8% of CD107a+CD8+ cells. Cytokine stimulation induced the production of IFN-γ in 30% of MAIT cells. After stimulation with E. coli, around 50% of MAIT cells produced TNF. About 40% of basophils responded to aFcƐR1 stimulation. Similar activation ranges were achieved i
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Leukemia and Lymphoma

Leukemia and Lymphoma | from Flow Cytometry to Cytomics | Scoop.it
Hematologic malignancies (blood cancers) are diseases of immune system cells and are traditionally categorized according to where the cancer is first detected.
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Jochen Behrends on LinkedIn: #cytolytics #cytolution #bat #allergy #basophils #diagnostic

Jochen Behrends on LinkedIn: #cytolytics #cytolution #bat #allergy #basophils #diagnostic | from Flow Cytometry to Cytomics | Scoop.it
Visualizing Basophil Activation in Allergy Diagnostics Using Cytolution

Cytolytics’ Cytolution software simplifies our flow cytometric data analysis and…
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Advances in liquid biopsy: From exploration to practical application

Marcus Jorelle's curator insight, December 13, 2024 6:07 AM
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Le score de Matutes revisité grâce à la cytométrie en flux multicouleurs standardisée !

Le score de Matutes revisité grâce à la cytométrie en flux multicouleurs standardisée ! | from Flow Cytometry to Cytomics | Scoop.it

Le score de Matutes, décrit par le Dr Matutes du Royal Marsden Hospital à Londres en 1994, est un score diagnostique de la leucémie lymphoïde chronique (LLC), la forme la plus fréquente de leucémie chronique chez l'adulte. Il est basé sur l'étude de l’expression, par cytomérie en flux, de 5 marqueurs de surface des lymphocytes B: le CD5, le CD23, le FMC7, le CD79b et l'intensité de l'immunoglobuline de surface. Un point est ou non attribué en fonction de l’expression de chaque marqueur, et un score supérieur ou égal à 4 est très en faveur du diagnostic de LLC. Dans les combinaisons d’anticorps utilisées en cytométrie en flux pour le diagnostic des hémopathies lymphoïdes B, est systématiquement présent le CD20 qui permet d'isoler la population B, c’est également une cible thérapeutique. L’anticorps anti FMC7 reconnaissant un épitope de la protéine CD20, il y a compétition entre les anticorps et l’étude du FMC7 nécessite donc un tube spécifique, actuellement en 8 couleurs/8 antigènes.

 

Les cytomètres de routine hospitalière passant de 8-10 couleurs à 12-13 couleurs, Valérie Bardet et ses collaborateurs de du Service d'Hématologie-Immunologie-Transfusion des Hôpitaux Universitaires Paris Ile de France-Ouest (UVSQ/UPSaclay, Boulogne-Billancourt) se sont demandés s'ils pouvaient remplacer l’étude du FMC7 par celle de l’intensité du CD20, ceci afin de limiter le nombre d’anticorps utilisés et donc le coût et le temps des techniques. Leur étude parue dans le British Journal of Haematology a été réalisée sur une cohorte de 508 patients atteints d’hémopathie lymphoïde B chronique provenant des hôpitaux Ambroise Paré et Saint Louis. Elle a permis de montrer qu’une nouvelle modalité de calcul était possible avec des performances égales au score historiquement décrit.

 

Légende Figure : Comparaison des résultats du calcul du score avec le FMC7 (barre de gauche) ou le CD20 (barre de droite) dans différents types d'hémopathies B (CLL, chronic lymphocytic leukaemia; DLBCL, diffuse large B‐cell lymphoma; FL, follicular lymphoma; HCL, hairy cell leukaemia; MCL, Mantle cell lymphoma; MZL, marginal zone lymphoma; SDRPL, splenic diffuse red pulp small B‐cell lymphoma; WM, Waldenström macroglobulinaemia)

 

Contact : valerie.bardet@aphp.fr 


Via Life Sciences UPSaclay
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https://www.tandfonline.com/doi/full/10.1080/19336918.2024.2395807#d1e312

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July 16, 2024 4:41 AM
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Flow cytometry as you have never seen it before

Presented By: Jordi Petriz

Speaker Biography: Jordi Petriz received his BSc degree in Biochemistry and Animal Biology from the University of Barcelona. He then pursued his PhD at Barcelona after acceptance at the Cryobiology and Cell Therapy Department, Cancer Research Institute (IRO)...

Webinar: Flow cytometry as you have never seen it before

Webinar Abstract: The Invitrogen™ Attune™ CytPix Flow Cytometer can simultaneously collect fluorescence data and high-resolution brightfield images of individual cells. This allows to better understand the morphology of each cell population identified for analysis. The images can be used to study cell-cell interactions, rare event identification, and rapid confirmation of disease...

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May 12, 2024 11:33 AM
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abcam on LinkedIn: The complete flow cytometry guide

abcam on LinkedIn: The complete flow cytometry guide | from Flow Cytometry to Cytomics | Scoop.it
Get everything you need to set up your flow and multicolor flow cytometry experiments with our comprehensive guide
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April 21, 2024 7:45 AM
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https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.24841

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February 22, 2024 4:21 AM
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Blood-based liquid biopsy: A promising noninvasive test in diagnosis, surveillance, and prognosis of patients with upper tract urothelial carcinoma

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February 13, 2024 11:08 AM
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Training an automated circulating tumor cell classifier when the true classification is uncertain | PNAS Nexus | Oxford Academic

Training an automated circulating tumor cell classifier when the true classification is uncertain | PNAS Nexus | Oxford Academic | from Flow Cytometry to Cytomics | Scoop.it
Abstract. Circulating tumor cells (CTC) and tumor-derived Extracellular Vesicles (tdEV) loads are prognostic of survival in carcinoma patients. The current meth
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February 2, 2024 8:18 AM
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Liquid Biopsy For Cancer Diagnostics Market Size, Growth And Forecast 2024-2033

Liquid Biopsy For Cancer Diagnostics Market Size, Growth And Forecast 2024-2033 | from Flow Cytometry to Cytomics | Scoop.it
Global liquid biopsy for cancer diagnostics market size is expected to reach $16.42 Bn by 2028 at a rate of 15.7%, segmented as by type, product, services...
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January 15, 2024 9:03 AM
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Rapid evaluation of T cell clonality in the diagnostic work-up of mature T cell neoplasms: TRBC1-based flow cytometric assay experience

highlights

  • Unimodal TRBC1 pattern correlates with T cell clonality.

  • A TRBC1-including multiparametric panel improves clonality assessment sensitivity.

  • Monitoring TRBC1 helps in identifying small T-cell clones of uncertain significance.

  • TRBC1 represents an opportunity to overcome time-consuming diagnostic strategies.

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December 9, 2023 9:57 AM
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Paolo Cappella, Ph.D sur LinkedIn : #flowcytometry | 40 commentaires

Paolo Cappella, Ph.D sur LinkedIn : #flowcytometry | 40 commentaires | from Flow Cytometry to Cytomics | Scoop.it
Season's Greetings 🎄

As we celebrate this festive season, i am excited to share the spirit of innovation and discovery with our valued community. This is… | 40 commentaires sur LinkedIn
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Chat GPT a encore frappé

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November 14, 2023 5:46 AM
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Ultra-sensitive CTC-based liquid biopsy for pancreatic cancer enabled by large blood volume analysis | Molecular Cancer | Full Text

Ultra-sensitive CTC-based liquid biopsy for pancreatic cancer enabled by large blood volume analysis | Molecular Cancer | Full Text | from Flow Cytometry to Cytomics | Scoop.it
The limited sensitivity of circulating tumor cell (CTC) detection in pancreatic adenocarcinoma (PDAC) stems from their extremely low concentration in the whole circulating blood, necessitating enhanced detection methodologies. This study sought to amplify assay-sensitivity by employing diagnostic leukapheresis (DLA) to screen large blood volumes. Sixty patients were subjected to DLA, with a median processed blood volume of ~ 2.8 L and approximately 5% of the resulting DLA-product analyzed using CellSearch (CS). Notably, DLA significantly increased CS-CTC detection to 44% in M0-patients and 74% in M1-patients, yielding a 60-fold increase in CS-CTC enumeration. DLA also provided sufficient CS-CTCs for genomic profiling, thereby delivering additional genomic information compared to tissue biopsy samples. DLA CS-CTCs exhibited a pronounced negative prognostic impact on overall survival (OS), evidenced by a reduction in OS from 28.6 to 8.5 months (univariate: p = 0.002; multivariable: p = 0.043). Additionally, a marked enhancement in sensitivity was achieved (by around 3-4-times) compared to peripheral blood (PB) samples, with positive predictive values for OS being preserved at around 90%. Prognostic relevance of CS-CTCs in PDAC was further validated in PB-samples from 228 PDAC patients, consolidating the established association between CTC-presence and reduced OS (8.5 vs. 19.0 months, p < 0.001). In conclusion, DLA-derived CS-CTCs may serve as a viable tool for identifying high-risk PDAC-patients and aiding the optimization of multimodal treatment strategies. Moreover, DLA enables comprehensive diagnostic profiling by providing ample CTC material, reinforcing its utility as a reliable liquid-biopsy approach. This high-volume liquid-biopsy strategy presents a potential pathway for enhancing clinical management in this malignancy.
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