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Scooped by
Gilbert C FAURE
February 22, 2024 4:21 AM
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Scooped by
Gilbert C FAURE
February 13, 2024 11:08 AM
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Abstract. Circulating tumor cells (CTC) and tumor-derived Extracellular Vesicles (tdEV) loads are prognostic of survival in carcinoma patients. The current meth
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Scooped by
Gilbert C FAURE
February 2, 2024 8:18 AM
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Global liquid biopsy for cancer diagnostics market size is expected to reach $16.42 Bn by 2028 at a rate of 15.7%, segmented as by type, product, services...
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Scooped by
Gilbert C FAURE
January 15, 2024 9:03 AM
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highlights -
Unimodal TRBC1 pattern correlates with T cell clonality. - •
A TRBC1-including multiparametric panel improves clonality assessment sensitivity. - •
Monitoring TRBC1 helps in identifying small T-cell clones of uncertain significance. - •
TRBC1 represents an opportunity to overcome time-consuming diagnostic strategies.
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Scooped by
Gilbert C FAURE
December 9, 2023 9:57 AM
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Season's Greetings 🎄
As we celebrate this festive season, i am excited to share the spirit of innovation and discovery with our valued community. This is… | 40 commentaires sur LinkedIn
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Scooped by
Gilbert C FAURE
November 14, 2023 5:46 AM
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The limited sensitivity of circulating tumor cell (CTC) detection in pancreatic adenocarcinoma (PDAC) stems from their extremely low concentration in the whole circulating blood, necessitating enhanced detection methodologies. This study sought to amplify assay-sensitivity by employing diagnostic leukapheresis (DLA) to screen large blood volumes. Sixty patients were subjected to DLA, with a median processed blood volume of ~ 2.8 L and approximately 5% of the resulting DLA-product analyzed using CellSearch (CS). Notably, DLA significantly increased CS-CTC detection to 44% in M0-patients and 74% in M1-patients, yielding a 60-fold increase in CS-CTC enumeration. DLA also provided sufficient CS-CTCs for genomic profiling, thereby delivering additional genomic information compared to tissue biopsy samples. DLA CS-CTCs exhibited a pronounced negative prognostic impact on overall survival (OS), evidenced by a reduction in OS from 28.6 to 8.5 months (univariate: p = 0.002; multivariable: p = 0.043). Additionally, a marked enhancement in sensitivity was achieved (by around 3-4-times) compared to peripheral blood (PB) samples, with positive predictive values for OS being preserved at around 90%. Prognostic relevance of CS-CTCs in PDAC was further validated in PB-samples from 228 PDAC patients, consolidating the established association between CTC-presence and reduced OS (8.5 vs. 19.0 months, p < 0.001). In conclusion, DLA-derived CS-CTCs may serve as a viable tool for identifying high-risk PDAC-patients and aiding the optimization of multimodal treatment strategies. Moreover, DLA enables comprehensive diagnostic profiling by providing ample CTC material, reinforcing its utility as a reliable liquid-biopsy approach. This high-volume liquid-biopsy strategy presents a potential pathway for enhancing clinical management in this malignancy.
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Scooped by
Gilbert C FAURE
October 3, 2023 8:28 AM
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World Experts. Your Colleagues. Join Us. You are cordially invited to attend ICCS 2023, the 38th Annual International Clinical Cytometry Meeting & Course. Please join your clinical cytometry colleagues from September 29 - October 3, 2023.
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Scooped by
Gilbert C FAURE
September 21, 2023 1:33 PM
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Cells, an international, peer-reviewed Open Access journal.
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Scooped by
Gilbert C FAURE
August 17, 2023 2:29 AM
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📢 What to know how cancer cells change their identity to evolve and escape therapy? Check out our latest review in Cancer Discovery that presents unifying…
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Scooped by
Gilbert C FAURE
July 2, 2023 3:57 AM
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Check the newest paper from Cíntia Marques, Olivier Jordan, Gerrit Borchard (he/him) from Institute of Pharmaceutical Sciences of Western Switzerland (ISPSO)-…
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Scooped by
Gilbert C FAURE
June 25, 2023 2:26 PM
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Tumorrestzellen: Standardisierte Flüssigbiopsien sollen Krebstherapie verbessern
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Scooped by
Gilbert C FAURE
June 16, 2023 8:23 AM
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According to the new market research report "Flow Cytometry Market Size, Growth by Technology (Cell-based, Bead-based), Product & Service (Analyzer, Sorter, Consumables, Software), Application ((Research - Immunology, Stem cell), (Clinical - Hematology)), End user (Biotech, Hospitals) - Global Forec...
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Scooped by
Gilbert C FAURE
April 19, 2023 2:46 AM
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A conversation with Gert Van Isterdael, Head of VIB Flow Core at the VIB-UGent Center for Inflammation Research
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Scooped by
Gilbert C FAURE
February 13, 2024 11:10 AM
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The Center for Bioactive Delivery's Pobezinsky and Pobezinskaya have analyzed and described what they call the "mosquito effect," shedding light on how certain pathogens can outwit the body's immune system.
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Scooped by
Gilbert C FAURE
February 6, 2024 3:53 AM
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#ExtraCellularVesicles | Impressively Comprehensive OPEN ACCESS Review Covering Role for #EVs in #Cancer #NeuroDegenerativeDiseases #Diabetes…
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Scooped by
Gilbert C FAURE
January 17, 2024 4:26 AM
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Scooped by
Gilbert C FAURE
January 2, 2024 8:09 AM
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BackgroundThere is a growing need for immunological assays to test toxic and modulatory effects of chemicals. The assays should be easy to use, reproducible and superior to cell line-based assays. We have therefore developed a comprehensive portfolio of assays based on primary human blood cells that are suitable for testing chemical effects.MethodsThe flow cytometry-based assays were designed to target a wide range of human peripheral blood mononuclear cells and whole blood, including T cells, NK cells, B cells, basophils and innate-like T cells such as γδT, MAIT and NKT cells. We have selected a set of activation markers for each immune cell, e.g: CD154 (T cells), CD137, CD107a (NK cells), CD63 (basophils), CD69, CD83 (B cells), CD69, IFN-γ (MAIT cells) and we selected cell specific stimuli: aCD3 antibodies (T cells); E. coli and cytokines IL-12/15/18 (MAIT cells); CpG ODN2006, R848 or aCD40 antibodies (B cells), fMLP or aFcϵR1 (basophils) or K562 cells (NK cells).ResultsBy selecting immune cell-specific markers and cell-specific stimuli, we were able to induce particular immune responses from the targeted immune cells. For example, the response to stimulation with anti-CD3 antibodies was in 36.8% of CD107a+CD8+ cells. Cytokine stimulation induced the production of IFN-γ in 30% of MAIT cells. After stimulation with E. coli, around 50% of MAIT cells produced TNF. About 40% of basophils responded to aFcƐR1 stimulation. Similar activation ranges were achieved i
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Scooped by
Gilbert C FAURE
November 21, 2023 5:22 AM
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Hematologic malignancies (blood cancers) are diseases of immune system cells and are traditionally categorized according to where the cancer is first detected.
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Scooped by
Gilbert C FAURE
October 5, 2023 2:43 AM
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The amount of data collected with cytometers keeps increasing. With studies measuring up to 40 markers, millions of cells per sample and up to thousands of samples, manual gating of all this data becomes infeasible. In this summer school, we will have workshops on all steps of a computational cytometry pipeline in R. On Monday morning, we offer two tracks, one for people with a cytometry background and one for people with a computational background. From Monday afternoon onwards, we will dive into the computational cytometry specifics. We will cover data cleaning, cell population identification and downstream statistics. In addition, we will also have some sessions where these concepts are demonstrated on commercial platforms, such as FlowJo, FCSExpress and OMIQ.
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Scooped by
Gilbert C FAURE
September 24, 2023 4:00 AM
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IntroductionThe three groups of helper innate lymphoid cells (ILCs), namely ILC1, ILC2 and ILC3, have been identified by flow cytometry by combinations of cell surface markers. Here, we review various ways ILCs are currently identified, focusing on potential problems and their solutions. The first step to identify all ILCs is to exclude other lymphocytes and myeloid cells by their lineage-specific markers (Lin). However, the Lin cocktail varies in various studies, and the definition of Lin- population containing ILCs is often ambiguous, resulting in contamination of Lin+ cells, particularly T cells.MethodWe have designed combinations of cell surface markers to identify ILC populations in various tissues of B6 mice by flow cytometry. To minimize T cell contamination, TCR/CD3ϵ antibodies were used separately from the Lin cocktail. ILCs identified by surface markers are confirmed by the expression of the transcription factors GATA3, RORγt, T-bet and Eomes.ResultILC1s in the B6 mouse liver are identified by Lin-NKp46+NK1.1+TCR/CD3ϵ−CD49a+CD49b−. However, defining ILC1s in other tissues remains a challenge. ILC2s in the lung are identified by Lin−TCR/CD3ϵ− Thy1+CD127+ST2+ whereas ILC2s in the small intestine and liver are identified by Lin−TCR/CD3ϵ−Thy1+GATA3+RORγt−. ILC3s in B6 mouse spleen, liver, lung and small intestine are identified by Lin−TCR/CD3ϵ− Thy1+CD127+RORγt+.DiscussionThe ILC population is heterogeneous and the strategies to identify ILCs have to be designed fo
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Scooped by
Gilbert C FAURE
September 5, 2023 2:39 AM
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CRUSTY is a web-tool for clustering analysis designed for high-dimensional single-cell data.
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Scooped by
Gilbert C FAURE
July 28, 2023 2:50 AM
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🩸 Delighted to share this article on the characterization of viable CTCs with you ! Good reading !!!! 💫
#liquidbiopsy #circulatingtumorcells #CTCs #cancer…
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Scooped by
Gilbert C FAURE
July 1, 2023 4:15 AM
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Your cytometry learning portal.
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Scooped by
Gilbert C FAURE
June 24, 2023 4:48 AM
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Probiotic enumeration is a key testing component to ensure reliable and compliant products reach consumers. Eurofins offers a unique ISO 17025 accredited flow cytometry method for probiotic enumeration. Flow cytometry offers faster turn-around times and reduced relative standard deviation compared to traditional plating methods. However, limitations also exist for flow cytometry applications.
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Scooped by
Gilbert C FAURE
May 13, 2023 4:57 AM
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Although immunotherapy is becoming a standard approach of human cancer treatment, only a small but critical fraction of patients responds to the therapy. It is therefore required to determine the sub-populations of patients who will respond to immunotherapies along with developing novel strategies...
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