Mucosal Immunity

Paper-based immunoassays, usually in the form of lateral flow tests, are currently the standard platform for home diagnostics. However, conventional lateral tests are often complicated by severe non-specific adsorption of detector particles when applied to test samples containing salivary fluid. It is believed that a high concentration of proteinaceous substances in salivary fluid causes particle aggregation and adhesion. In this study, we developed a stacking flow platform for single-step detection of a target antibody in salivary fluid. Stacking flow circumvents the need for separate sample pre-treatments, such as filtration or centrifugation, which are often required prior to testing saliva samples using paper-based immunoassays. This is achieved by guiding the samples and reagents to the test strip through different paths. By doing so, salivary substances that interfere with the particle-based sensing system are removed before they come into contact with the detection reagents, which greatly reduces the background. In addition, the stacking flow configuration enables uniform flow with a unique flow regulator, which leads to even test lines with good quantification capability, enabling the detection of ~20 ng mL−1 α-fetoprotein in the serum. We have successfully applied the stacking flow device to detect dengue-specific immunoglobulins that are present in salivary fluid.

by M. Reza Zariffard, Kathryn Anastos, Audrey L. French, Elisaphane Munyazesa, Mardge Cohen, Alan L. Landay, Gregory T.

Swedish researchers may have found a solution to the growing resistance to antibiotics in the most unlikely of places — breastmilk.

Primary gastric lymphoma accounts for 7% of primary gastric neoplasms . H. pylori infection is considered to be the ultimate cause of gastric lymphomas

by Sébastien Boutin, Simon Y. Graeber, Michael Weitnauer, Jessica Panitz, Mirjam Stahl, Diana Clausznitzer, Lars Kaderali, Gisli Einarsson, Michael M. Tunney, J. Stuart Elborn, Marcus A. Mall, Alexander H.