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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world. 
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If you're going to Brazil for the World Cup, don’t forget your insect repellent, and maybe one of these. The country has the highest incidence of dengue fever in the world, with...
BigField GEG Tech's insight:
Oxitec has developed transgenic mosquitoes. The modified male mosquitoes are released into the wild and create progeny that have the lethal gene.
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The rise of antibiotic resistance rekindles interest in a century-old virus treatment.
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The tangled highway of blood vessels that twists and turns inside our bodies, delivering essential nutrients and disposing of hazardous waste to keep our organs working properly has been a conundrum for scientists trying to make artificial vessels from scratch.
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Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.
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In a new study published in the peer reviewed Public Library of Science (PLOS), researchers emphasize that there is sufficient evidence that meal-derived DNA fragments carry complete genes that can enter into the human circulation system through an unknown mechanism. (0) I wonder if the scientists at these biotech corporations have already identified this method? […]
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Although two high-profile research articles on stimulus-triggered acquisition of pluripotency (STAP) cells were found to be flawed in an interim investigatory report by Riken, the state-affiliated institute stopped short of ...
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
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PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in phiC31-mediated integration in different types of mammalian cells; as a result, the efficiencies of pseudo attP site integration and evaluation of site-specific integration are compromised. To improve this system, we used an attB-TK fusion gene as a negative selection marker, thereby eliminating random integration during phiC31-mediated transfection. We also excised the selection system and plasmid bacterial backbone by using two other site-specific recombinases, Cre and Dre. Thus, we generated clean transgenic bovine fetal fibroblast cells free of selectable marker and plasmid bacterial backbone. These clean cells were used as donor nuclei for somatic cell nuclear transfer (SCNT), indicating a similar developmental competence of SCNT embryos to that of non-transgenic cells. Therefore, the present gene delivery system facilitated the development of gene therapy and agricultural biotechnology.
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BigField GEG Tech
March 10, 2014 5:53 AM
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In such fungi, RNAi has been induced by expressing hairpin RNAs delivered through plasmids, sequences integrated in fungal or plant genomes, or by RNAi generated in planta by a plant virus… ...
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BigField GEG Tech
June 10, 2014 10:09 AM
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A couple of years ago, my fiancée and I wanted to try to make some home-made mozzarella cheese, but ran into a problem. In order to ...
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BigField GEG Tech
June 6, 2014 11:06 AM
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Genetic engineering of plants, animals and microorganisms such as bacteria typically involves the use of restriction enzymes to 'cut and paste' DNA fragments into certain genetic sequence locations. This process allows scientists to introduce new genes into an organism, but is constrained to specific ...
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BigField GEG Tech
June 6, 2014 10:40 AM
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Artist prints 3D replica of van Gogh's ear grown from living cells of his great-great grandson.
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BigField GEG Tech
June 4, 2014 6:22 AM
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Proof of concept of how PhiC31 recombinase works in Capra hircus fibroblasts. Moreover, this study points out new pseudo attP sites in the mammalian genome.
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BigField GEG Tech
May 27, 2014 10:00 AM
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ChIP-seq for Cas9 shows varying amounts of off-target binding with different guide RNAs and low levels of indels at some off-target sites.
CRISPR Cas had recently emerged as a new tool for DNA engineering, It's quite promising due to the easy of use and the reduced cost compared to TALENs or ZFNs. However we are still waiting for robust challenging of this technology, espacially regarding the off-target frequency. The team of Mazhar Adli provides new insights on this question and bring elements to decipher the factors that may influence the targeting specificity.
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BigField GEG Tech
May 5, 2014 10:26 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Genetic engineering of iPs invloving 3 site specific recombinases. PhiC31 integrase was used to mediate initial placement of a single copy of a reprogramming plasmid into the genome at a safe location. A second phage integrase, Bxb1, was used to place the full-length dystrophin coding sequence into the same location, and Cre resolvase was utilized to excise unwanted sequences.
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BigField GEG Tech
April 29, 2014 10:49 AM
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The fusion Cas9/FokI enhances specificity of CRISPR system without reduce the efficiency. In human cells, the authors shown that this fusion allows the modification of target DNA sites with a 140-fold higher specificity than wild-type Cas9 and with an efficiency similar to that of paired Cas9 'nickases'.
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BigField GEG Tech
March 19, 2014 10:07 AM
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PubMed comprises more than 23 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
CRISPR to modify HPSC by homologous recombination, and one use of more for this technology!
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BigField GEG Tech
March 12, 2014 4:39 AM
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The first high resolution three-dimensional structure of the DNA modifying enzyme Cas9. Nice!
Smart strategy which allows a very simple, efficient and fast assay for detecting induced mutations It will be surely widely used in the area of genome editing.
http://geg-tech.com/