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Genetic Engineering Publications - GEG Tech top picks
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In a new study published in the peer reviewed Public Library of Science (PLOS), researchers emphasize that there is sufficient evidence that meal-derived DNA fragments carry complete genes that can enter into the human circulation system through an unknown mechanism. (0) I wonder if the scientists at these biotech corporations have already identified this method? […]
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Although two high-profile research articles on stimulus-triggered acquisition of pluripotency (STAP) cells were found to be flawed in an interim investigatory report by Riken, the state-affiliated institute stopped short of ...
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
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PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in phiC31-mediated integration in different types of mammalian cells; as a result, the efficiencies of pseudo attP site integration and evaluation of site-specific integration are compromised. To improve this system, we used an attB-TK fusion gene as a negative selection marker, thereby eliminating random integration during phiC31-mediated transfection. We also excised the selection system and plasmid bacterial backbone by using two other site-specific recombinases, Cre and Dre. Thus, we generated clean transgenic bovine fetal fibroblast cells free of selectable marker and plasmid bacterial backbone. These clean cells were used as donor nuclei for somatic cell nuclear transfer (SCNT), indicating a similar developmental competence of SCNT embryos to that of non-transgenic cells. Therefore, the present gene delivery system facilitated the development of gene therapy and agricultural biotechnology.
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BigField GEG Tech
March 10, 2014 5:53 AM
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In such fungi, RNAi has been induced by expressing hairpin RNAs delivered through plasmids, sequences integrated in fungal or plant genomes, or by RNAi generated in planta by a plant virus… ...
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BigField GEG Tech
March 5, 2014 3:39 AM
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A general protocol for the generation of Nanobodies for structural biology http://t.co/AYYkCzgeKp
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The opening of a new way of research which seems promising!
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BigField GEG Tech
February 25, 2014 5:28 AM
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A paper diagnostic for cancer: Low-cost urine test amplifies signals from growing tumors to detect disease. It will be great!
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BigField GEG Tech
February 24, 2014 9:14 AM
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Researchers at the Gladstone Institutes use gene editing to move a step closer to genetic disease cure.
BigField GEG Tech's insight:
A new method to capture rare mutational events, such as single-base substitutions without antibiotic selection. Nice step for the genome editing field!
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BigField GEG Tech
February 12, 2014 6:00 AM
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Nanorobots into human cells! The beginning of fantastic voyages?
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BigField GEG Tech
June 4, 2014 6:22 AM
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PhiC31 Integrase Induces Efficient Site-Specific Recombination in the Capra hircus Genome | Abstract
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Proof of concept of how PhiC31 recombinase works in Capra hircus fibroblasts. Moreover, this study points out new pseudo attP sites in the mammalian genome.
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BigField GEG Tech
May 27, 2014 10:00 AM
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ChIP-seq for Cas9 shows varying amounts of off-target binding with different guide RNAs and low levels of indels at some off-target sites.
BigField GEG Tech's insight:
CRISPR Cas had recently emerged as a new tool for DNA engineering, It's quite promising due to the easy of use and the reduced cost compared to TALENs or ZFNs. However we are still waiting for robust challenging of this technology, espacially regarding the off-target frequency. The team of Mazhar Adli provides new insights on this question and bring elements to decipher the factors that may influence the targeting specificity.
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BigField GEG Tech
May 5, 2014 10:26 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
BigField GEG Tech's insight:
Genetic engineering of iPs invloving 3 site specific recombinases. PhiC31 integrase was used to mediate initial placement of a single copy of a reprogramming plasmid into the genome at a safe location. A second phage integrase, Bxb1, was used to place the full-length dystrophin coding sequence into the same location, and Cre resolvase was utilized to excise unwanted sequences.
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BigField GEG Tech
April 29, 2014 10:49 AM
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The fusion Cas9/FokI enhances specificity of CRISPR system without reduce the efficiency. In human cells, the authors shown that this fusion allows the modification of target DNA sites with a 140-fold higher specificity than wild-type Cas9 and with an efficiency similar to that of paired Cas9 'nickases'.
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BigField GEG Tech
March 19, 2014 10:07 AM
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PubMed comprises more than 23 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
BigField GEG Tech's insight:
CRISPR to modify HPSC by homologous recombination, and one use of more for this technology!
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March 12, 2014 4:39 AM
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The first high resolution three-dimensional structure of the DNA modifying enzyme Cas9. Nice!
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March 7, 2014 3:49 AM
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Synthetic spider silk strong enough for a superhero, the research pipeline for synthetic spider silk is very active, and scientists expect that production is right on the verge of scaling up.
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February 24, 2014 9:36 AM
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Scientists studying the sexual transformation of the malaria parasite have solved a long-standing mystery in parasite biology. Two research teams have independently discovered that a single protein acts as the master genetic switch that triggers the development of male and female sexual forms of the malaria parasite. The research also gives important clues for identifying the underlying mechanisms that control this developmental fate, determining whether or not a malaria parasite will be able to transmit the disease. The discovery has important implications for human health.
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Stéphanie Philippe
from Tracking the Future
February 15, 2014 6:51 AM
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Over the last decade, as DNA-sequencing technology has grown ever faster and cheaper, our understanding of the human genome has increased accordingly. Yet scientists have until recently remained largely ham-fisted when they’ve tried to directly modify genes in a living cell. Take sickle-cell anemia, for example. A debilitating and often deadly disease, it is caused by a mutation in just one of a patient’s three billion DNA base pairs. Even though this genetic error is simple and well studied, researchers are helpless to correct it and halt its devastating effects.
Now there is hope in the form of new genome-engineering tools, particularly one called CRISPR. This technology could allow researchers to perform microsurgery on genes, precisely and easily changing a DNA sequence at exact locations on a chromosome. Along with a technique called TALENs, invented several years ago, and a slightly older predecessor based on molecules called zinc finger nucleases, CRISPR could make gene therapies more broadly applicable, providing remedies for simple genetic disorders like sickle-cell anemia and eventually even leading to cures for more complex diseases involving multiple genes. Most conventional gene therapies crudely place new genetic material at a random location in the cell and can only add a gene. In contrast, CRISPR and the other new tools also give scientists a precise way to delete and edit specific bits of DNA—even by changing a single base pair. This means they can rewrite the human genome at will.
Via Szabolcs Kósa
BigField GEG Tech's insight:
Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.
http://geg-tech.com/