 Your new post is loading...
Your new post is loading...
|
Scooped by
BigField GEG Tech
June 17, 2024 5:05 AM
|
A team at Montana State University published research this week that shows how RNA, the close chemical cousin to DNA, can be edited using CRISPRs.
|
|
Scooped by
BigField GEG Tech
April 24, 2024 5:41 AM
|
The bioengineered immune players called CAR T cells last longer and work better if pumped up with a large dose of a protein that makes them resemble stem cells.
|
|
Scooped by
BigField GEG Tech
April 12, 2024 6:58 AM
|
Since its breakthrough development more than a decade ago, CRISPR has revolutionized DNA editing across a broad range of fields.
|
|
Scooped by
BigField GEG Tech
April 2, 2024 7:26 AM
|
Magnetic resonance imaging (MRI) and lumbar puncture (LP) may not always be necessary for diagnosing and managing a serious neurological complication associated with CAR T-cell therapy, according to a new Blood Advances study.
|
|
Scooped by
BigField GEG Tech
March 21, 2024 6:48 AM
|
In vivo CRISPR genome-wide screening pinpoints the transcriptional modulator CITED2 as a pivotal driver in the progression of prostate cancer to bone metastasis.
|
|
Scooped by
BigField GEG Tech
March 11, 2024 6:11 AM
|
Over the past two decades, the immune system has attracted increasing attention for its role in fighting cancer.
|
|
Scooped by
BigField GEG Tech
March 6, 2024 10:52 AM
|
Current approaches for inserting autonomous transgenes into the genome, such as CRISPR–Cas9 or virus-based strategies, have limitations including low efficiency and high risk of untargeted genome mutagenesis. Here, we describe precise RNA-mediated insertion of transgenes (PRINT), an approach for site-specifically primed reverse transcription that directs transgene synthesis directly into the genome at a multicopy safe-harbor locus. PRINT uses delivery of two in vitro transcribed RNAs: messenger RNA encoding avian R2 retroelement-protein and template RNA encoding a transgene of length validated up to 4 kb. The R2 protein coordinately recognizes the target site, nicks one strand at a precise location and primes complementary DNA synthesis for stable transgene insertion. With a cultured human primary cell line, over 50% of cells can gain several 2 kb transgenes, of which more than 50% are full-length. PRINT advantages include no extragenomic DNA, limiting risk of deleterious mutagenesis and innate immune responses, and the relatively low cost, rapid production and scalability of RNA-only delivery. Transgenes are inserted into human cells by 2-RNA delivery of a retroelement protein and template.
|
|
Scooped by
BigField GEG Tech
February 7, 2024 6:07 AM
|
study evaluates senolytic CAR T-cell therapy targeting uPAR-positive cells in aged mice, showing its effectiveness in mitigating age-related metabolic dysfunction and offering a potential long-lasting treatment for aging-associated conditions.
|
|
Scooped by
BigField GEG Tech
February 5, 2024 6:11 AM
|
A new light-inducible RNA base editing tool, padCas13, combines the specificity of CRISPR-Cas13 with the control of light activation and allows for precise, reversible RNA targeting and degradation in mammalian cells, both in vitro and in vivo.
|
|
Scooped by
BigField GEG Tech
February 1, 2024 11:56 AM
|
Using CRISPR, an immune system bacteria use to protect themselves from viruses, scientists have harnessed the power to edit genetic information within cells.
|
|
Scooped by
BigField GEG Tech
January 17, 2024 6:06 AM
|
Researchers developed an RNA-based switch, the pA regulator system, to control gene expression in mammalian cells by modulating synthetic polyA signal cleavage, offering a novel approach for gene therapy applications.
|
|
Scooped by
BigField GEG Tech
December 26, 2023 10:36 AM
|
Utilizing CRISPR screening, the deubiquitinase ATXN3 has been identified as a key regulator of PD-L1 transcription in tumor cells, a critical factor in tumor immune evasion.
|
|
Scooped by
BigField GEG Tech
December 20, 2023 9:00 AM
|
|
|
|
Scooped by
BigField GEG Tech
June 17, 2024 4:43 AM
|
A team at Montana State University published research this week that shows how RNA, the close chemical cousin to DNA, can be edited using CRISPRs.
|
|
Scooped by
BigField GEG Tech
April 15, 2024 9:55 AM
|
Mayo Clinic scientists have developed an immunotherapy strategy that potentially lays the groundwork for treating a spectrum of autoimmune diseases.
|
|
Scooped by
BigField GEG Tech
April 11, 2024 9:22 AM
|
Treatment with a next-generation CAR T-cell agent displayed early efficacy in a small group of patients with glioblastoma.
|
|
Scooped by
BigField GEG Tech
March 29, 2024 7:05 AM
|
Targeting two brain tumor-associated proteins-;rather than one-;with CAR T cell therapy shows promise as a strategy for reducing solid tumor growth in patients with recurrent glioblastoma (GBM), an aggressive form of brain cancer, according to early results from the first six patients treated in an ongoing Phase I clinical trial led by researchers from the Perelman School of Medicine at the University of Pennsylvania and Penn Medicine's Abramson Cancer Center.
|
|
Scooped by
BigField GEG Tech
March 13, 2024 8:21 AM
|
Fluorescence resonance energy transfer (FRET) reporters are commonly used in the final stages of nucleic acid amplification tests to indicate the presence of nucleic acid targets, where fluorescence is restored by nucleases that cleave the FRET reporters. However, the need for dual labelling and purification during manufacturing contributes to the high cost of FRET reporters. Here we demonstrate a low-cost silver nanocluster reporter that does not rely on FRET as the on/off switching mechanism, but rather on a cluster transformation process that leads to fluorescence color change upon nuclease digestion. Notably, a 90 nm red shift in emission is observed upon reporter cleavage, a result unattainable by a simple donor-quencher FRET reporter. Electrospray ionization–mass spectrometry results suggest that the stoichiometric change of the silver nanoclusters from Ag13 (in the intact DNA host) to Ag10 (in the fragments) is probably responsible for the emission colour change observed after reporter digestion. Our results demonstrate that DNA-templated silver nanocluster probes can be versatile reporters for detecting nuclease activities and provide insights into the interactions between nucleases and metallo-DNA nanomaterials. Here the authors present a non-FRET DNA-templated silver nanocluster probe that exhibits a distinct colour switch from green to red upon nuclease digestion, visible under UV excitation, offering a low-cost, effective alternative to fluorescent reporters for detecting nuclease activities.
|
|
Scooped by
BigField GEG Tech
March 7, 2024 9:49 AM
|
Siteman Cancer Center, based at Barnes-Jewish Hospital and Washington University School of Medicine in St. Louis, is one of the first centers nationwide to offer a newly approved cell-based immunotherapy that targets melanoma.
|
|
Scooped by
BigField GEG Tech
March 4, 2024 6:23 AM
|
Review synthesizes research on NK cells' role in cancer immunity and their potential in therapeutics through bioengineering, immune checkpoint inhibitors, and cell engagers, highlighting ongoing preclinical and clinical trials.
|
|
Scooped by
BigField GEG Tech
February 6, 2024 5:58 AM
|
This study is led by Prof. Xianqun Fan (Department of Ophthalmology, Shanghai Jiao Tong University School of Medicine Affiliated Ninth People's Hospital).
|
|
Scooped by
BigField GEG Tech
February 2, 2024 6:13 AM
|
Chimeric antigen receptor T-cell therapy, or CAR T, has dramatically improved the treatment of certain blood cancers. Initially approved for patients who had failed multiple lines of therapy, clinical trials have shown CAR T can be used as an earlier treatment option.
|
|
Scooped by
BigField GEG Tech
January 31, 2024 9:10 AM
|
|
|
Scooped by
BigField GEG Tech
January 16, 2024 6:27 AM
|
At EPFL's School of Engineering, Professor Li Tang's Laboratory of Biomaterials for Immunoengineering has made significant strides in cancer treatment research.
|
|
Scooped by
BigField GEG Tech
December 21, 2023 6:14 AM
|
The recent publication in Science by Mogila, Tamulaitiene et al. represents a continuation of the successful scientific research conducted by Gintautas Tamulaitis' group.
|
Scoop.it!
RNA repair could be a fundamental aspect of biology, and harnessing this activity could lead to new life-saving cures. In particular, RNA editing has important applications in the search for treatments for genetic diseases. A team of researchers recently published research showing how RNA can be edited using a different CRISPR system to that for DNA, called type III. The study article entitled "Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells" was published in the journal Science and is the latest advance in the team's ongoing exploration of CRISPR applications for programmable genetic engineering.