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Scooped by
Ed Rybicki
January 15, 2020 4:00 AM
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Introduction
Annual influenza vaccination is recommended for older adults, but evidence regarding the impact of repeated vaccination has been inconclusive. Aim We investigated vaccine effectiveness (VE) against laboratory-confirmed influenza and the impact of repeated vaccination over 10 previous...
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Scooped by
Ed Rybicki
December 11, 2019 5:15 AM
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Scientists braved the heat to answer a question about oxygen and "Snowball Earth," a period when the oceans virtually froze from the poles to the equator.
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Scooped by
Ed Rybicki
December 11, 2019 5:14 AM
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Researchers from the University of South Australia are close to developing a life-changing vaccine for deadly peanut allergies.
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Scooped by
Ed Rybicki
December 11, 2019 5:11 AM
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Lyon, France – December 5, 2019 – Osivax announced today that it has entered into an agreement with the United States’ National Institute of Allergy and Infectious Diseases (NIAID), part of...
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Ed Rybicki
December 11, 2019 5:10 AM
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An Aboriginal elder in Queensland is working with a team of researchers to find mass graves — the final resting place of 90 locals who died in the space of three weeks during one of the world's worst outbreaks of Spanish influenza.
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Scooped by
Ed Rybicki
December 11, 2019 5:09 AM
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Flu Shot(alt-text) "Wait, how often are you getting bitten by snakes? And why are you boiling water?" "Dunno, the CDC people keep showing up with complicated questions about the 'history of the property' and 'possible curses' but I kinda tune them out. At least one of them offered me the flu shot." |< < ? > >| You can get it here: Amazon, B&N, IndieBound, Apple, Audible, Target Archive Store What If? About
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Scooped by
Ed Rybicki
December 11, 2019 5:08 AM
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It's that time of year when we should think about the grandparents' vaccines before they meet and hold newborns.And also these vaccines protect them...
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Scooped by
Ed Rybicki
December 11, 2019 5:07 AM
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A man with asthma who caught the flu ended up in a coma after declining a flu vaccine.
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Scooped by
Ed Rybicki
December 11, 2019 5:06 AM
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Scientists call some cancers "Wnt addicted" because they can't live without it. New research with gastric cancer organoids suggests a new treatment strategy.
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Scooped by
Ed Rybicki
December 11, 2019 5:06 AM
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"A safety signal could be a musical piece, a person, or even an item like a stuffed animal that represents the absence of threat."...
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Ed Rybicki
December 11, 2019 5:04 AM
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Watching the way rat brains replay their memories forward and backward while making a decision let researchers predict what it would decide.
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Scooped by
Ed Rybicki
December 3, 2019 5:05 AM
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KISS have been forced to cancel their entire Australian tour as Paul Stanley continues to battle a bad bout of influenza.
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Scooped by
Ed Rybicki
December 3, 2019 5:04 AM
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According to social media posts, flu shots contain 25 micrograms of mercury, despite the safe limit for injected mercury being zero. This is misleading; of the nine flu vaccines authorized in Canada this year, only five contain thimerosal, a mercury-containing organic compound used to preserve...
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Scooped by
Ed Rybicki
January 15, 2020 3:57 AM
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PCV3 capsid protein (Cap) is an important antigen for diagnosis and vaccine development. To achieve high-level expression of recombinant PCV3 Cap in Escherichia coli (E. coli), the gene of wild-type entire Cap (wt-eCap) was amplified from clinical samples, and three optimized entire Cap (opti-eCap) and one optimized Cap deleted nuclear location signal (NLS) (opti-dCap) gene fragments encoding the same amino acid sequence with wt-eCap were synthesized based on the codon bias of E. coli. Those gene fragments were inserted into the pET30a expression vector. One recombinant strain with the highest expressed soluble eCap from four entire Cap (one wt-eCap and three opti-eCap) and one recombinant strain expressed opti-dCap were selected for further purification. The purified eCap and dCap were identified by transmission electron microscopy (TEM), a large number of round hollow particles with a diameter of 10 nm virus-like particles (VLPs) were observed in eCap, whereas irregular aggregation of proteins observed in dCap. After formation the VLPs were applied as a coating antigen to establish an indirect ELISA (I-ELISA) for detection of PCV3-specific antibody in swine serum. 373 clinical swine serum samples from China collected in 2019 were tested utilizing the VLP-based I-ELISA method under optimized conditions. To the best of our knowledge, this is the first report of self-assembly into VLPs of PCV3 recombinant Cap. Our results demonstrated that the VLP-based I-ELISA will be a valuable tool for detecting the presence of PCV3 antibodies in serum samples and will facilitate screening of large numbers of swine serum for clinical purposes.
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Scooped by
Ed Rybicki
December 11, 2019 5:15 AM
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New evidence points to an giant, icy planet orbiting a white dwarf star that's slowing evaporating it.
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Scooped by
Ed Rybicki
December 11, 2019 5:13 AM
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Preparing for the Next Pandemic Given the ongoing threat posed by influenza, the WHO earlier this year released its Global Influenza Strategy 2019–2030. Although the strategy is a welcome step, add...
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Scooped by
Ed Rybicki
December 11, 2019 5:11 AM
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- A stockpile of 500,000 doses of Ebola vaccine for emergency use in outbreaks o...
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Scooped by
Ed Rybicki
December 11, 2019 5:10 AM
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There’s a good chance flu season could peak much earlier than normal in the U.S. this winter, health officials say.
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Scooped by
Ed Rybicki
December 11, 2019 5:09 AM
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Norovirus Antibody May Make Effective “Stomach Flu” Vaccine Genetic Engineering & Biotechnology News...
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Scooped by
Ed Rybicki
December 11, 2019 5:08 AM
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The first observations from NASA's Parker Solar Probe "will fundamentally change our understanding of the sun," researchers say.
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Scooped by
Ed Rybicki
December 11, 2019 5:07 AM
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"Among Bennu's many surprises, the particle ejections sparked our curiosity, and we've spent the last several months investigating this mystery."...
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Scooped by
Ed Rybicki
December 11, 2019 5:06 AM
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A new early life intranasal vaccine will allow UK cattle farmers can now protect dairy and beef calves against the key causes of viral pneumonia from as young as one week of age.
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Scooped by
Ed Rybicki
December 11, 2019 5:05 AM
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By NewsDesk @bactiman63 Taiwan health officials reported today (computer translated) on two human H9N2 avian influenza on Mainland China. The cases, from November 12th and 22nd, are the first reported in China this season.
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Scooped by
Ed Rybicki
December 11, 2019 5:03 AM
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BACKGROUND: Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVADs). The infection of PCV2 is widespread and has serious consequence, thereby causing significant economic losses in the swine industry worldwide. Previously, we found that a strain named YiY-3-2-3 has a naturally occurring point mutation (G710 to A710) in ORF1 region, which leads to a shorten product of the rep gene (945 to 660 base pair). Importantly, the Rep protein is responsible for genome replication of PCV2. To explore the effects of this mutation on the PCV2 replication, in the current study we constructed infectious clone of this IF-YiY-3-2-3, as well as those of its two parental strains of IF-YiY-3-2-1 and IF-YiY-3-2-10. Subsequently, these infectious clones which have 1.1 copy of PCV2 genome of their corresponding strains were transfected into PK15 cells to obtain rescued viruses, respectively. RESULTS: Though all of the three infectious clones could be rescued, the copy number and infectivity of these rescued viruses were significantly different, as analyzed by fluorescence quantitative PCR, Tissue culture infectious dose 50 (TCID50), and indirect immunofluorescence assay (IFA). Notably, whether the PCV2 copy number, viral titer or the infectivity of rescued viruses from infectious clone IF-YiY-3-2-3 was significantly less than those of its parental clones. Meanwhile, the spatial structure of the Rep protein from the IF-YiY-3-2-3 displayed an apparent truncation at the C-terminal. CONCLUSIONS: These findings therefore suggest that the Rep protein with truncated C-terminal would reduce virus replication and infectivity, and there might also exist both favorable and unfavorable mutations in the ORF1 of PCV2 in the process of its evolution.
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Scooped by
Ed Rybicki
December 3, 2019 5:04 AM
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Scientists have puzzled over the existence of buckyballs in space, but a new idea about how the carbon molecules form could solve the mystery.
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