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Truncated Rep protein of porcine circovirus 2 (PCV2) caused by a naturally occurring mutation reduced virus replication in PK15 cells

BACKGROUND: Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVADs). The infection of PCV2 is widespread and has serious consequence, thereby causing significant economic losses in the swine industry worldwide. Previously, we found that a strain named YiY-3-2-3 has a naturally occurring point mutation (G710 to A710) in ORF1 region, which leads to a shorten product of the rep gene (945 to 660 base pair). Importantly, the Rep protein is responsible for genome replication of PCV2. To explore the effects of this mutation on the PCV2 replication, in the current study we constructed infectious clone of this IF-YiY-3-2-3, as well as those of its two parental strains of IF-YiY-3-2-1 and IF-YiY-3-2-10. Subsequently, these infectious clones which have 1.1 copy of PCV2 genome of their corresponding strains were transfected into PK15 cells to obtain rescued viruses, respectively. RESULTS: Though all of the three infectious clones could be rescued, the copy number and infectivity of these rescued viruses were significantly different, as analyzed by fluorescence quantitative PCR, Tissue culture infectious dose 50 (TCID50), and indirect immunofluorescence assay (IFA). Notably, whether the PCV2 copy number, viral titer or the infectivity of rescued viruses from infectious clone IF-YiY-3-2-3 was significantly less than those of its parental clones. Meanwhile, the spatial structure of the Rep protein from the IF-YiY-3-2-3 displayed an apparent truncation at the C-terminal. CONCLUSIONS: These findings therefore suggest that the Rep protein with truncated C-terminal would reduce virus replication and infectivity, and there might also exist both favorable and unfavorable mutations in the ORF1 of PCV2 in the process of its evolution.

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Virology News
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Why the Covid-19 coronavirus is worse than the flu, in one chart

Why the Covid-19 coronavirus is worse than the flu, in one chart | Virology News | Scoop.it
It’s more contagious, more deadly (particularly for older people), and it has a greater potential to overwhelm our health care system.
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GREAT graphic!!
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Oldest human genetic data may expand our 'family tree'

Oldest human genetic data may expand our 'family tree' | Virology News | Scoop.it
The oldest human genetic data ever recovered could shed light on the evolution of our ancestors and clarify the branches of the human family tree.
Ed Rybicki's insight:
Hey, It's not ALL about SARS2!
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At the frontlines of Nigeria’s COVID-19 response

At the frontlines of Nigeria’s COVID-19 response | Virology News | Scoop.it
Ultra-low freezers hum and beep continuously in the biorespository room, supporting the non-stop diagnostic activities at the National Public Health Reference Laboratory in Gaduwa, Abuja, on a relatively quiet Friday afternoon.
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COVID-19's economic effects could last decades

COVID-19's economic effects could last decades | Virology News | Scoop.it
A look back at the economic effect of past pandemics shows that we could be facing COVID-19's impact for years and years to come.
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US flu activity drops sharply; death toll holds at 24K

US flu activity drops sharply; death toll holds at 24K | Virology News | Scoop.it
While the U.S. flu season is ebbing, it’s still not over.
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Anthony Fauci says it's likely coronavirus will become 'seasonal'

Anthony Fauci says it's likely coronavirus will become 'seasonal' | Virology News | Scoop.it
Dr. Anthony Fauci, an infectious disease expert on the White House task force, said Sunday it is likely that coronavirus will become a seasonal illness.
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Presymptomatic Transmission of SARS-CoV-2 — Singapore, January 23–March 16, 2020

Presymptomatic Transmission of SARS-CoV-2 — Singapore, January 23–March 16, 2020 | Virology News | Scoop.it
Investigation of all 243 cases of COVID-19 reported in Singapore during January 23–March 16 identified seven clusters of cases in which presymptomatic transmission is the most likely explanation for the occurrence of secondary cases.
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Novel coronavirus vaccine being tested on rodents in Israel 

Novel coronavirus vaccine being tested on rodents in Israel  | Virology News | Scoop.it
The institute's director told Prime Minister Benjamin Netanyahu that the vaccine was being readied for testing in animals.
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Ed Rybicki's comment, April 2, 5:53 AM
And yet again, they don't say what it is B-(
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Researchers team up to design face shield kits

Researchers team up to design face shield kits | Virology News | Scoop.it
"These face shields are a key component of the equipment that helps keep our providers safe while caring for patients during this critical time."...
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Australia begins pre-clinical testing for virus vaccine

Australia begins pre-clinical testing for virus vaccine | Virology News | Scoop.it
Australia's national science agency has said that it has commenced the first stage of testing potential vaccines for Covid-19, as it joins a global race to halt the coronavirus pandemic.
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A Simplified Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants

It's not all about COVID B-)I

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A Vaccine From The 1920s [BCG: Ed]  Is Now Being Tested For Use Against The Coronavirus Pandemic

A Vaccine From The 1920s [BCG: Ed]  Is Now Being Tested For Use Against The Coronavirus Pandemic | Virology News | Scoop.it
Researchers in a handful of countries are testing a century-old tuberculosis vaccine to see if it can give a boost to the immune system to help it fight off the novel coronavirus now causing the COVID-19 pandemic.
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China's restrictions may have prevented 700K COVID-19 cases

China's restrictions may have prevented 700K COVID-19 cases | Virology News | Scoop.it
"...without the Wuhan travel ban and the national emergency response there would have been more than 700,000 confirmed COVID-19 cases outside of Wuhan..."...
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Some people who recover from the coronavirus might be left with '20 to 30%' less lung function, and gasping for breath when they walk quickly, Hong Kong doctors said

Some people who recover from the coronavirus might be left with '20 to 30%' less lung function, and gasping for breath when they walk quickly, Hong Kong doctors said | Virology News | Scoop.it
The Hong Kong Hospital Authority made the discovery after observing the first wave of discharged patients in the city.
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Bill Lapschies, Survivor of Spanish Flu, Recovers From Coronavirus

Bill Lapschies, Survivor of Spanish Flu, Recovers From Coronavirus | Virology News | Scoop.it
World War II vet Bill Lapschies was feted on his 104th birthday Wednesday, also celebrating his recovery from the coronavirus.
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Coronavirus Australia: $230 million boost for CSIRO's COVID vaccine push

Coronavirus Australia: $230 million boost for CSIRO's COVID vaccine push | Virology News | Scoop.it
Government will spend $220m to upgrade the Geelong lab currently testing two vaccines for COVID-19, Science Minister Karen Andrews will announce Saturday.
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Ferrets, hamsters will soon reveal whether Canadian vaccine bid has a shot

Ferrets, hamsters will soon reveal whether Canadian vaccine bid has a shot | Virology News | Scoop.it
Saskatchewan lab finding ways to fast-track research amid COVID-19 pandemic...
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Coronavirus: Anti-Parasite Drug Ivermectin Kills Coronavirus Within 48 Hours In Lab Settings, Claims Report

Coronavirus: Anti-Parasite Drug Ivermectin Kills Coronavirus Within 48 Hours In Lab Settings, Claims Report | Virology News | Scoop.it
Amid a barrage of research on finding treatment for new coronavirus, Australian scientists have found that a common anti-parasitic drug killed SARS-CoV-2 virus, growing in cell culture, within 48 hours in lab settings. Ivermectin is an FDA-approved anti-parasitic drug that has also been shown to be effective in vitro against a broad range of viruses including HIV, dengue, influenza and zika virus. Published in the journal Antiviral Research, the study from Monash University showed that a single dose of Ivermectin could stop the coronavirus growing in cell culture -- effectively eradicating all genetic material of the virus within two days. "We found that even a single dose could essentially remove all viral RNA by 48 hours and that even at 24 hours there was a really significant reduction in it," said study lead author Dr Kylie Wagstaff. Dr Wagstaff, however, cautioned that the tests conducted in the study were in vitro and that trials needed to be carried out in people. "Ivermectin is very widely used and seen as a safe drug. We need to figure out now whether the dosage you can use it at in humans will be effective - that''s the next step," Wagstaff informed. In times when we're having a global pandemic and there isn't an approved treatment, "if we had a compound that was already available around the world then that might help people sooner". "Realistically it's going to be a while before a vaccine is broadly available," she said. Although the mechanism by which Ivermectin works on the virus is not known, it is likely, based on its action in other viruses, that it works to stop the virus ''dampening down'' the host cells' ability to clear it. Dr Wagstaff made a previous breakthrough finding on Ivermectin in 2012 when she identified the drug and its antiviral activity with Monash Biomedicine Discovery Institute's Professor David Jans, also an author on this paper. Professor Jans and his team have been researching Ivermectin for more than 10 years with different viruses. Dr Wagstaff and Professor Jans started investigating whether it worked on the SARS-CoV-2 virus as soon as the pandemic was known to have started. The use of Ivermectin to combat COVID-19 depends on pre-clinical testing and clinical trials, with funding urgently required to progress the work, the researchers noted. (This story has been published from a wire agency feed without modifications to the text. Only the headline has been changed.)
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Team tracks coronavirus in wastewater

Team tracks coronavirus in wastewater | Virology News | Scoop.it
Testing for the new coronavirus in wastewater to trace its prevalence in US communities could help public health officials better prepare for the future.
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See Bill Gates’ Chilling Pandemic Warnings To Trump – Before The Coronavirus Outbreak Hit 

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COVID-19 precautions are impossible in prisons

COVID-19 precautions are impossible in prisons | Virology News | Scoop.it
"A prison in a pandemic becomes akin to a cruise ship—people are crowded into a shared space that they cannot leave." Here are prisons' COVID-19 options.
Ed Rybicki's insight:
We have a large and very crowded prison population in South Africa. It could get really bad here.
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Coronavirus: Saskatoon lab tests potential vaccine on ferrets

Coronavirus: Saskatoon lab tests potential vaccine on ferrets | Virology News | Scoop.it
VIDO-InterVac said it has isolated the coronavirus in its lab and generated a vaccine that it's now testing on ferrets.
Ed Rybicki's comment, April 2, 5:53 AM
No indication of what the vaccine is!!
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This is no April Fool's. British American Tobacco is trying to make a coronavirus vaccine

This is no April Fool's. British American Tobacco is trying to make a coronavirus vaccine | Virology News | Scoop.it
The maker of Camel, Lucky Strike and Pall Mall cigarettes is trying its hand at a new product: a coronavirus vaccine.
Ed Rybicki's insight:
Sigh...they say BAT, and then they show KBP's sign! They were making drugs / candidate vaccines in plants LONG before BAT  / RJ Reynolds bought them.
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Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein | Virology News | Scoop.it
The emergence of SARS-CoV-2 has resulted in >90,000 infections and >3,000 deaths.Coronavirus spike (S) glycoproteins promote entry into cells and are…...
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Influenza and Coronavirus Demise Could Lie with Phage Nanoparticles

Influenza and Coronavirus Demise Could Lie with Phage Nanoparticles | Virology News | Scoop.it
Researchers have found a new use for phage in the fight against seasonal influenza and avian flu and is being looked at to uses against coronavirus.
Ed Rybicki's insight:
Sigh...it's hype, of course, and I don't see widespread use of this ever becoming a Thing, but it IS cool B-)
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Prediction: a Vaccine before Christmas

Prediction: a Vaccine before Christmas | Virology News | Scoop.it
Three days ago, I asked our community to predict when the FDA would approve the first COVID-19 vaccine. What did the crowd say?
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