SynBioFromLeukipposInstitute
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Scooped by Gerd Moe-Behrens
March 23, 2015 11:51 AM
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The DIY biohacking revolution is here

The DIY biohacking revolution is here | SynBioFromLeukipposInstitute | Scoop.it
It's never been easier to build your own biological creations.
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Scooped by Gerd Moe-Behrens
March 23, 2015 11:49 AM
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Can We Capture the World's Data in Our DNA?

Can We Capture the World's Data in Our DNA? | SynBioFromLeukipposInstitute | Scoop.it
Does our DNA hold the key to creating one of the most long lasting and efficient storage devices around? Scientists certainly seem to think so.
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Scooped by Gerd Moe-Behrens
March 23, 2015 11:48 AM
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Nanocrafter: a Synthetic Biology Game | CodonOps

Nanocrafter: a Synthetic Biology Game | CodonOps | SynBioFromLeukipposInstitute | Scoop.it
Nanocrafter: a Synthetic Biology Game
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Scooped by Gerd Moe-Behrens
March 23, 2015 11:47 AM
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CEIES on Twitter

CEIES on Twitter | SynBioFromLeukipposInstitute | Scoop.it
Seminar announcement
Using Engineering Principles for Synthetic Biology Applications pic.twitter.com/vwokEYw646
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Scooped by Gerd Moe-Behrens
December 22, 2014 10:15 AM
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Advancement of Emerging Tools in Synthetic Biology for the Designing and Characterization of Genetic Circuits - Springer

Advancement of Emerging Tools in Synthetic Biology for the Designing and Characterization of Genetic Circuits - Springer | SynBioFromLeukipposInstitute | Scoop.it
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Scooped by Gerd Moe-Behrens
December 22, 2014 10:10 AM
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Synthetic Biology of Multicellular Systems: New Platforms and Applications for Animal Cells and Organisms

Synthetic Biology of Multicellular Systems: New Platforms and Applications for Animal Cells and Organisms | SynBioFromLeukipposInstitute | Scoop.it
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Scooped by Gerd Moe-Behrens
December 22, 2014 9:56 AM
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Victor de Lorenzo´s lab: Synthetic Biology meets Environmental Biotechnology for a better world - YouTube

How do bacteria sense their environment? How can we describe the genetic mechanisms behind this process? How can we use this knowledge to develop useful appl...
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Scooped by Gerd Moe-Behrens
December 22, 2014 9:32 AM
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CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells

CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells | SynBioFromLeukipposInstitute | Scoop.it
Gerd Moe-Behrens's insight:

by
Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y1, Kang T1 Bleris L

"Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fragments of the delivery vehicle. A crucial element of the proposed system is the CRISPR protein Cas9. Upon delivery, Cas9 guided by a custom RNA sequence cleaves the delivery vector at strategically placed targets thereby inactivating a co-expressed gene of interest. Importantly, using experiments in human embryonic kidney cells, we show that specific parameters of the system can be adjusted to fine-tune the delivery properties. We envision future applications in complex synthetic biology architectures, gene therapy and trace-free delivery."


 http://bit.ly/1GOvYUp

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December 19, 2014 10:34 AM
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Programmable RNA recognition and cleavage by CRISPR/Cas9

Programmable RNA recognition and cleavage by CRISPR/Cas9 | SynBioFromLeukipposInstitute | Scoop.it
stimulate
Gerd Moe-Behrens's insight:

by
Mitchell R. O’Connell, Benjamin L. Oakes, Samuel H. Sternberg, Alexandra East-Seletsky, Matias Kaplan & Jennifer A. Doudna

"The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA–DNA complementarity to identify target sites for sequence-specific double-stranded DNA (dsDNA) cleavage1, 2, 3, 4, 5. In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require recognition of a short DNA sequence, known as the protospacer adjacent motif (PAM), next to and on the strand opposite the twenty-nucleotide target site in dsDNA4, 5, 6, 7. Cas9 has proven to be a versatile tool for genome engineering and gene regulation in a large range of prokaryotic and eukaryotic cell types, and in whole organisms8, but it has been thought to be incapable of targeting RNA5. Here we show that Cas9 binds with high affinity to single-stranded RNA (ssRNA) targets matching the Cas9-associated guide RNA sequence when the PAM is presented in trans as a separate DNA oligonucleotide. Furthermore, PAM-presenting oligonucleotides (PAMmers) stimulate site-specific endonucleolytic cleavage of ssRNA targets, similar to PAM-mediated stimulation of Cas9-catalysed DNA cleavage7. Using specially designed PAMmers, Cas9 can be specifically directed to bind or cut RNA targets while avoiding corresponding DNA sequences, and we demonstrate that this strategy enables the isolation of a specific endogenous messenger RNA from cells. These results reveal a fundamental connection between PAM binding and substrate selection by Cas9, and highlight the utility of Cas9 for programmable transcript recognition without the need for tags."

http://bit.ly/13kZBNX

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December 19, 2014 10:26 AM
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Frontiers | Developments in the Tools and Methodologies of Synthetic Biology

Gerd Moe-Behrens's insight:

by
Kelwick R, MacDonald JT, Webb AJ, Freemont P.

"Synthetic biology is principally concerned with the rational design and engineering of biologically based parts, devices, or systems. However, biological systems are generally complex and unpredictable, and are therefore, intrinsically difficult to engineer. In order to address these fundamental challenges, synthetic biology is aiming to unify a "body of knowledge" from several foundational scientific fields, within the context of a set of engineering principles. This shift in perspective is enabling synthetic biologists to address complexity, such that robust biological systems can be designed, assembled, and tested as part of a biological design cycle. The design cycle takes a forward-design approach in which a biological system is specified, modeled, analyzed, assembled, and its functionality tested. At each stage of the design cycle, an expanding repertoire of tools is being developed. In this review, we highlight several of these tools in terms of their applications and benefits to the synthetic biology community."

http://bit.ly/1zHrWLy

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Scooped by Gerd Moe-Behrens
December 19, 2014 10:05 AM
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MoMA | Designing Life: Synthetic Biology and Design

MoMA | Designing Life: Synthetic Biology and Design | SynBioFromLeukipposInstitute | Scoop.it
MoMA | Designing Life: Synthetic Biology and Design
Luciana Santos's curator insight, December 20, 2014 2:19 PM

O que acontece quando a biologia e mais especificamente , os materiais nucleicos e os processos que sustentam o ciclo de vida de todos os seres vivos desde o nascimento , à existência , à doença e à morte torna-se sinteticamente replicáveis por seres humanos e consequentemente se tornam projetos de design ? Na sequência da recente publicação MIT Estética sintética e apenas alguns dias antes do  iGEM (International Máquinas Geneticamente Modificadas ) Biologia Sintética 2014 Jamboree no início de Novembro de 2014, o Moma se propôs a discutir esta complexa questão, hospedando um painel de discussão, Estética sintéticas: Novas Fronteiras da Contemporary Design.

Scooped by Gerd Moe-Behrens
December 19, 2014 10:04 AM
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Synthetic biology on a piece of paper: Brighter future for disease diagnostics? - Genetic Literacy Project

Synthetic biology on a piece of paper: Brighter future for disease diagnostics? - Genetic Literacy Project | SynBioFromLeukipposInstitute | Scoop.it
A new technology that allows synthetic gene circuits to be printed on paper could allow us to cheaply and accurately detect diseases.
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Scooped by Gerd Moe-Behrens
December 19, 2014 10:00 AM
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Synthetic biology: Toehold gene switches make big footprints

Synthetic biology: Toehold gene switches make big footprints | SynBioFromLeukipposInstitute | Scoop.it
Gerd Moe-Behrens's insight:

by
Simon Ausländer & Martin Fussenegger

"The development of RNA-based devices called toehold switches that regulate translation might usher in an era in which protein production can be linked to almost any RNA input and provide precise, low-cost diagnostics."


http://bit.ly/1DRsNNN

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Scooped by Gerd Moe-Behrens
March 23, 2015 11:50 AM
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Synthetic biology yields new approach to gene therapy

Synthetic biology yields new approach to gene therapy | SynBioFromLeukipposInstitute | Scoop.it
Bioengineers at The University of Texas at Dallas have created a novel gene-delivery system that shuttles a gene into a cell, but only for a temporary stay, providing a potential new gene-therapy strategy for treating disease.
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Scooped by Gerd Moe-Behrens
March 23, 2015 11:49 AM
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Democratizing synthetic biology: New method makes research cheaper, faster, and more accessible

Democratizing synthetic biology: New method makes research cheaper, faster, and more accessible | SynBioFromLeukipposInstitute | Scoop.it
Deep in the heart of synthetic biology are the proteins that make it tick. Protein engineering is the crucial pulse of the booming, relatively new scientific discipline.
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Rescooped by Gerd Moe-Behrens from Synthetic biology
March 23, 2015 11:48 AM
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Design and build of synthetic DNA goes back to 'BASIC'

Design and build of synthetic DNA goes back to 'BASIC' | SynBioFromLeukipposInstitute | Scoop.it

A new technique for creating artificial DNA that is faster, more accurate and more flexible than existing methods has been developed by scientists.


Via Integrated DNA Technologies
Scooped by Gerd Moe-Behrens
February 20, 2015 4:37 PM
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Programmable materials and the nature of the DNA bond

Programmable materials and the nature of the DNA bond | SynBioFromLeukipposInstitute | Scoop.it
Gerd Moe-Behrens's insight:

*Programmable materials and the nature of the DNA bond*

by
Matthew R. Jones, Nadrian C. Seeman2, Chad A. Mirkin

"BACKGROUND

Nucleic acids are ubiquitous in biology because of their ability to encode vast amounts of information via canonical Watson-Crick base-pairing interactions. With the advent of chemical methods to make synthetic oligonucleotides of an arbitrary sequence, researchers can program entire libraries of molecules with orthogonal interactions, directed to assemble in highly specific arrangements. Early attempts to use DNA to make nanostructures led to topologically defined architectures, but ones that were too conformationally flexible to be used to guide the construction of well-defined nanoscale materials from the bottom up. In this Review, we discuss the key discoveries that have overcome this limitation and distill common design principles that have since led to a revolution in materials sophistication based on DNA-directed assembly.
ADVANCES
The experimental realization of DNA-based constructs that are sufficiently rigid so as to impart directionality to hybridization interactions marks a major milestone in the development of programmable materials assembly. This feat was accomplished simultaneously by the Mirkin Group and Seeman Group in 1996, but through chemically and conceptually distinct pathways. In one approach, rigidity is derived from multiple strand crossover events and the hybridization that stabilizes them to create a conformationally restricted DNA tile. In the other approach, a rigid non-nucleic acid–based nanoparticle (inorganic or organic) core acts as a template to organize functionalized DNA strands in a surface-normal orientation. It is appealing to draw the analogy between DNA-based constructs of this sort with the concepts of “bonds” and “valency” found in atomic systems. Just as understanding the nature of atomic bonding is crucial for chemists to manipulate the formation of molecular and supramolecular species, so too is an understanding of the nature of these DNA bonding modes necessary for nanoscientists to build complex and functional architectures to address materials needs.
OUTLOOK
The interest in nanoscale materials constructed by using DNA bonds has continued to grow steadily, but has seen a noteworthy explosion in relevance over the past several years. This is due in large part to the development of methods to move beyond simple clusters and crystals to more sophisticated nanostructured materials that are dynamic and stimuli responsive, are macroscopic in spatial extent, and exhibit emergent physical properties that arise from specific arrangements of matter. These techniques offer perhaps the most versatile way of organizing optically active materials into architectures that exhibit unusual and deliberately tailorable plasmonic and photonic properties. In addition, prospects include the use of these materials in biological settings, being that they are constructed, in large measure, from nucleic acid precursors. The ability to manipulate gene expression, deliver molecular payloads via DNA-based binding events, and detect relevant markers of disease with nanoscale spatial resolution represent some of the most fruitful avenues of future research."


http://bit.ly/1EyPgeD

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Scooped by Gerd Moe-Behrens
December 22, 2014 10:12 AM
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Book Review of Synthetic Biology: A Lab Manual

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Scooped by Gerd Moe-Behrens
December 22, 2014 10:01 AM
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Bacteria are wishing you a Merry Xmas

Bacteria are wishing you a Merry Xmas | SynBioFromLeukipposInstitute | Scoop.it
A bacterium has been used to wish people a Merry Xmas. Grown by Dr Munehiro Asally, an Assistant Professor at the University of Warwick, the letters used to spell MERRY XMAS are made of Bacillus subtilis, a non-pathogenic bacterium which is found in soil and also human gut.
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Scooped by Gerd Moe-Behrens
December 22, 2014 9:56 AM
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Media Lab Conversations Series: George Church

Media Lab Conversations Series: George Church | SynBioFromLeukipposInstitute | Scoop.it
synthetic biology: inventing intelligent design http://t.co/Saiw6ZL4WR
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Scooped by Gerd Moe-Behrens
December 19, 2014 10:52 AM
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New class of synthetic molecules mimics antibodies

New class of synthetic molecules mimics antibodies | SynBioFromLeukipposInstitute | Scoop.it
A Yale University lab has crafted the first synthetic molecules that have both the targeting and response functions of antibodies.
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Scooped by Gerd Moe-Behrens
December 19, 2014 10:29 AM
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Computational Methods in Synthetic Biology - Springer

Computational Methods in Synthetic Biology - Springer | SynBioFromLeukipposInstitute | Scoop.it
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Scooped by Gerd Moe-Behrens
December 19, 2014 10:20 AM
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▶ Synthetic Aesthetics: New Frontiers in Contemporary Design

Paola Antonelli, Director of R&D and Senior Curator, Department of Architecture and Design, MoMA, introduces the symposium Synthetic Aesthetics: New Frontiers in Contemporary Design, an investigation of the intersections between synthetic biology and design.

The symposium features guest speakers David Benjamin, Daisy Ginsberg, Dan Grushkin, and William Shih.
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December 19, 2014 10:05 AM
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George Church on the Future of Human Genomics and Synthetic Biology - YouTube

http://research.ncsu.edu/ges In his talk “The Future of Human Genomics and Synthetic Biology,” Church discussed the exponentially fast pace of emerging genet...
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Scooped by Gerd Moe-Behrens
December 19, 2014 10:04 AM
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Biocoding For Beginners

Biocoding For Beginners | SynBioFromLeukipposInstitute | Scoop.it
How you can build a biotech startup for less than a mobile app.
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