Immunology Diagnosis
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Important considerations when planning to measure the levels of cytokines and chemokines in serum or plasma.
Alfredo Corell's insight:
Circulating levels of these proteins or other chemistries are measured from plasma or serum collected from peripheral blood draws. It is important to note that the methods of sampling and storage of plasma or serum are critical for accurate measurements. 
BrainImmune's comment,
September 25, 2013 7:26 AM
Thanks for this important considerations - few people pay attention to these details
Alfredo Corell's comment,
September 26, 2013 1:31 PM
cytokines are very labile and unstable. These considerations, as you well know, are criticall for any research or diagnostic purpouse.
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Genetic profiling to identify specific cell surface markers for a highly suppressive Treg population.
Alfredo Corell's insight:
Tr1 cells uniquely expressed genes for two cell surface markers, CD49b and LAG-3.
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For more information, visit http://www.bio-rad.com/yt/6/biotech-lab-textbook. This video demonstrates the Ouchterlony double immunodiffusion assay. This tech... Via Manuel Garcîa
Alfredo Corell's insight:
an step-to-step viedo showing the Ouchterlony Assay
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Programa de Garantía Externa de Calidad para Laboratorios de Inmunología Diagnóstica
Alfredo Corell's insight:
Mínimo precio - Gran calidad Comienzo inminente el 10 de junio de 2013 plazas disponibles
Instrucciones de inscripción: http://www.geclidsei.uva.es/mod/resource/view.php?id=2773
Alfredo Corell's curator insight,
June 9, 2013 2:55 PM
Programa de los cursos y metodologia docente: http://www.geclidsei.uva.es/mod/resource/view.php?id=3165
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For more info, visit http://www.bio-rad.com/yt/CellCounting. Eliminate the tedium of manual cell counting with Bio-Rad's TC20 automated cell counter. The TC2...
Alfredo Corell's insight:
An automated, reproducible and realible cell counter that simplifies your cell counting work on the lab
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Website for the 27th European Immunogenetics and Histocompatibility Conference, 11-14 May 2013, Maastricht, The Netherlands
Alfredo Corell's insight:
GECLID-SEI posters: innovation & research about Immunogenetics Intercomparisons (quality proficiency testing)
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Streck Introduces Flow Cytometry Control for CD117 read Streck Laboratories Inc. news in the SelectScience scientific news archive
Alfredo Corell's insight:
CD check for abnormal CD117 expresión control
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I came across this interesting infographic by MDG advertising this morning and thought I’d share it with you.
Alfredo Corell's insight:
Is this change to digital diagnosis of enough quality and safe??
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Medical Laboratory Observer Amid indications that vitamin D deficiency is increasing, clinical laboratories should conduct an informed consideration of available test options.
Alfredo Corell's insight:
The results reported for a total vitamin D assay may differ based on the method used. Immunoassay methods report a single total vitamin D result; high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) methods report vitamin D2 and vitamin D3 values separately and the two values are added together to obtain the total vitamin D value. Current scientific literature does not indicate that there is a clinical advantage to the latter approach, as it is the total 25(OH)D value that drives a clinician’s decision to treat.
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Alfredo Corell's insight:
ESCCA Summer Schools represent our new policy towards the development of residential, full-immersion courses which will pave the way to the specific certification of technical and academical operators, widely felt as a fundamental step for laboratory accreditation. Part of the courses will be organized nationally, in local languages and in full collaboration with the local cytometric society. An International course will be also organized in English, for the benefit of the operators from smaller Countries that cannot enjoy the support of a local society.
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Este año 2013 tendra lugar la celebración de un evento con actividades infantiles el día 28 de Abril.
Alfredo Corell's insight:
Toda la información sobre eventos para adultos y niños que tendrán lugar el 28 de abril de 2013 en la Maternidad de O'Donell
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Alfredo Corell's insight:
Even though DVD players are becoming all but obsolete due to the availability of USB drives and video streaming, their cheap optics may find a new life in a cost-effective, speedy technique for on-the-spot HIV testing and other analytics.
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El IOBA es un Instituto marcado por la excelencia en muchas líneas de trabajo, en las que es un auténtico referente internaciona. Entre ellas, el ámbito de la Inmunología. Como ejemplo, baste recordar que este laboratorio dirigido por Alfredo Corell, desarrolla un proyecto pionero mundial para garantizar un mejor diagnóstico previo en los trasplantes de órganos.
Alfredo Corell's insight:
Enlace al artículo en pdf: http://www.psc.uva.es/pdf_dossier/2013/06/0016SPMJ.pdf
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A Multiplexed Approach for Immunogenicity Assessment Using the SQiDlite System - Case Study Analysis
From
xtalks
In collaboration with SQI Diagnostics, Algorithme Pharma offers a unique multiplexed immunoassay system to characterize Anti-Drug-Antibody (ADA) using a reporter cocktail of differentially labeled secondary antibodies.
Alfredo Corell's insight:
Registration link: https://www2.gotomeeting.com/register/205925202
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Alfredo Corell's insight:
The important role of lab-diagnosis in disease discovery and follow-up
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Programa de Garantía Externa de Calidad para Laboratorios de Inmunología Diagnóstica
Alfredo Corell's insight:
Mínimo precio - Gran calidad Comienzo inminente el 10 de junio de 2013 plazas disponibles
Instrucciones de inscripción: http://www.geclidsei.uva.es/mod/resource/view.php?id=2773
Alfredo Corell's comment,
June 9, 2013 2:37 PM
No puedes adjuntar una copia de tu resguardo de pago con el boletín de inscripción. Pero se lo puedes mandar a nuestros administrativos en geclid-sei@ioba.med.uva.es
Alfredo Corell's curator insight,
June 9, 2013 2:55 PM
Programa de los cursos y metodologia docente: http://www.geclidsei.uva.es/mod/resource/view.php?id=3165
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La Universidad de Valladolid y las sociedades de Citometría e Inmunología han firmado este jueves un convenio que contempla la colaboración entre las tres instituciones para mejorar el diagnóstico en los transplantes de órganos y enfermedades como...
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Create flow cytometry settings that allow for standardized consistent fluorescence intensity target values.
Alfredo Corell's insight:
Guidelines and steps to standarize Flow cytometry.
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Alfredo Corell's insight:
Registration is in full swing and space is limited. The tentative registration deadline is May 17th. In order to guarantee your spot please complete your registration!
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Measuring proliferation of lymphocytes using CFSE is not a foolproof protocol.
Alfredo Corell's insight:
A simple and useful review about This cytometry-based technique to asses lymphocyte proliferation
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Alfredo Corell's insight:
BMJ Qual Saf doi:10.1136/bmjqs-2012-001550 Original research25-Year summary of US malpractice claims for diagnostic errors 1986–2010: an analysis from the National Practitioner Data Bank
Ali S Saber Tehrani1,HeeWon Lee2,Simon C Mathews2,Andrew Shore3,Martin A Makary3,Peter J Pronovost4,David E Newman-Toker1
Conclusions Among malpractice claims, diagnostic errors appear to be the most common, most costly and most dangerous of medical mistakes. We found roughly equal numbers of lethal and non-lethal errors in our analysis, suggesting that the public health burden of diagnostic errors could be twice that previously estimated. Healthcare stakeholders should consider diagnostic safety a critical health policy issue.
a review in a blog: http://qualityhealthcareplease.wordpress.com/2013/04/24/diagnostic-errors-symptom-to-treatment-disconnect/
Review at Doctorslounge: http://www.doctorslounge.com/index.php/news/pb/37467
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Visit our online shop: http://www.nitzipper.com nitzipper® is a new and revolutionary bioconjugation technology that allows to quickly, easily, and effective...
Alfredo Corell's insight:
Conjugate any particle or biomolecule with the conjugate you need for your research or diagnostic purpouses
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This is the concluding lecture about the T cells. Function of T helper 1, and 2, cytotoxic T cells, NK cells and T regulator cells (T17) are discussed.
Alfredo Corell's insight:
By Mobeen Syed part 1: http://www.youtube.com/watch?v=GKf8-Ex6F7Y&list=TLv1_K8sf8xWw part 2: http://www.youtube.com/watch?v=j7vlkt93UEc&list=TLv1_K8sf8xWw Part 3: http://www.youtube.com/watch?v=wap9Ilm7HPE&list=TLv1_K8sf8xWw part 4: http://www.youtube.com/watch?v=u5O2avcbkzY&list=TLv1_K8sf8xWw part 5: http://www.youtube.com/watch?v=PLCPjFsD8Kc&list=TLv1_K8sf8xWw
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UCLA professor Dr. Aydogan Ozcan and his team have created a compact, low-cost fluorescent microscope and flow cytometer out of a cell phone camera.
Alfredo Corell's insight:
While you’re running around taking silly photos with your smartphone of what you ate for lunch today, a researcher at the University of California, Los Angeles, has found a way to photograph microscopic cell samples using a cell phone camera. Dr. Aydogan Ozcan, a professor and head of the Ozcan Research Group at UCLA, and his team of electrical scientists and bioengineers have developed a device that fits onto any camera-equipped cell phone that could benefit doctors and scientists in countries with limited clinical supplies. The research findings were recently published in the Journal of Visualized Experiments(JoVE, subscription required). Read more: http://www.digitaltrends.com/photography/ucla-scientists-turn-lowly-cell-phone-camera-into-fully-functional-research-microscope/#ixzz2QooMoN2V Follow us: @digitaltrends on Twitter | digitaltrendsftw on Facebook
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The literature on diagnostic tests for food allergy currently lacks clear consensus regarding the accuracy and safety of different investigative approaches. The European Academy of Allergy and Clinical Immunology is in the process of developing its Guideline for Food Allergy and Anaphylaxis, and this systematic review is one of seven inter-linked evidence syntheses that are being undertaken in order to provide a state-of-the-art synopsis of the current evidence base in relation to epidemiology, prevention, diagnosis and clinical management, and impact on quality of life, which will be used to inform the formulation of clinical recommendations. The aim of this systematic review will be to assess the diagnostic accuracy of tests aimed at supporting the clinical diagnosis of IgE-mediated food allergy.
link to the provisional pdf: http://www.ctajournal.com/content/pdf/2045-7022-3-18.pdf