Immunology Diagnosis
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Immunology Diagnosis

Immunology Diagnosis

Diagnostico en el Laboratorio de Inmunología
Curated by Alfredo Corell
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October 27, 2013 6:57 PM
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Across the Globe, ISO 15189 Enjoys Growing Acceptance by Governments for the Accreditation of Medical Laboratories in their Countries | Dark Daily

Across the Globe, ISO 15189 Enjoys Growing Acceptance by Governments for the Accreditation of Medical Laboratories in their Countries | Dark Daily | Immunology Diagnosis | Scoop.it
From www.darkdaily.com - October 27, 2013 6:57 PM
Even as only a small number of clinical laboratories in the United States have taken the steps to earn accreditation to ISO 15189 Medical Laboratories, it is a different story in other nations, particularly where no statutory requirement for...
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October 21, 2013 5:29 PM
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Primera Inmunopíldora en diagnóstico: producción de anticuerpos monoclonales

From www.youtube.com - October 21, 2013 5:29 PM
T.2 TÉCNICAS DE INMUNODIAGNÓSTICO. Inmunología Humana. Alfredo Corell Almuzara. Universidad de Valladolid. Contenidos: * Preparación de anticuerpos para diag...
Alfredo Corell's insight:

Hoy se ha publicado la primera píldora de la serie T, dedicada a técnicas de diagnóstico en el laboratorio de Inmunología.

 

Muchas gracias por seguir apoyando este proyecto

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October 13, 2013 9:01 AM
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ERA Significantly Accelerates Turnaround Time for Proficiency Testing Results

ERA Significantly Accelerates Turnaround Time for Proficiency Testing Results | Immunology Diagnosis | Scoop.it
From www.labbulletin.com - October 13, 2013 9:01 AM
New Process Reduces Waiting Period from Twenty-one Days to Two Days Environmental Resource Associates (ERA), a Waters® Business, recently completed a nine-month project to significantly reduce the turnaround time for reporting laboratory...
Alfredo Corell's insight:

It is imperative that laboratories receive reliable PT results as quickly as possible. Laboratories rely on proficiency tests to signal potential quality issues within their facility. A failed proficiency test could be an indicator of a problem with testing methodology, instrument, or an analyst’s performance.

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October 7, 2013 6:14 PM
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Alergólogos reúnen a más de 80 médicos de otras especialidades para intercambiar protocolos asistenciales

Alergólogos reúnen a más de 80 médicos de otras especialidades para intercambiar protocolos asistenciales | Immunology Diagnosis | Scoop.it
From www.elnortedecastilla.es - October 7, 2013 6:14 PM
Armentia: «Tenemos un gran equipo, que hace diagnóstico y tratamiento de alto nivel»
Alfredo Corell's insight:

Ver página de la Sociedad Castellano-Leonesa de Alergia e Inmunología Clínica, presidida por la Dra. Alicia Armentia

www.sclaic.es 

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October 5, 2013 2:08 PM
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Flow Cytometry Buying Guide

Flow Cytometry Buying Guide | Immunology Diagnosis | Scoop.it
From www.selectscience.net - October 5, 2013 2:08 PM
If you are looking to invest in Flow Cytometry technology, this guide aims to educate you on the essential information needed to assist your decisions.
Alfredo Corell's insight:

I've beem reading some parts of the document and I would rather expect a less tendentious results

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September 29, 2013 12:13 PM
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2013 Core Facility Benchmarking Study Results

2013 Core Facility Benchmarking Study Results | Immunology Diagnosis | Scoop.it
From www.ilabsolutions.com - September 29, 2013 12:13 PM
iLab Solutions today announced the results of the 2013 Core Facility Benchmarking Study.
Alfredo Corell's insight:

Boston - iLab Solutions announced today the results of its 3rd Annual Core Facility Benchmarking Study. This study is based on a survey of individuals who directly manage cores, service centers, shared facilities, and recharge centers at hospitals, universities, and independent research institutions. They represent more than 40 different core types from over 60 institutions from North America, Europe and the Asia Pacific region. The goals of the study are to provide an understanding of core facility management operations practices, trends in core growth and utilization, and the biggest challenges Core Managers face today.


Download the full 2013 Core Facility Benchmarking Study here:

http://www.ilabsolutions.com/wp-content/uploads/2013/09/20130830-BMS2013.pdf

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September 22, 2013 2:29 PM
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Immuno-PCR: molecular and Immunological techniques combined in DNA amplification

Immuno-PCR: molecular and Immunological techniques combined in DNA amplification | Immunology Diagnosis | Scoop.it
From www.innovabiosciences.com - September 22, 2013 2:29 PM
Introduction Since the first immunoassays were developed in the 1950's the variety of assay formats has increased dramatically.
Alfredo Corell's insight:

This new method, termed immuno-polymerase chain reaction (immuno-PCR), benefits from the specificity of antibodies and the sensitivity of PCR. Several independent studies undertaken since the publication of this seminal paper have consistently demonstrated the advantages of this technique over traditional ELISA, some achieving more than a 50,000 fold increase in sensitivity over the equivalent immunoassay [2]. Furthermore, immuno-PCR can be used in a multiplex format and offers accurate quantitation.

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September 22, 2013 12:40 PM
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Zellkraftwerk: chipcytometry promising 41-plex immunophenotype in September

Zellkraftwerk: chipcytometry promising 41-plex immunophenotype in September | Immunology Diagnosis | Scoop.it
From www.zellkraftwerk.com - September 22, 2013 12:40 PM
A new dimension in cytometry and immunohistology
Alfredo Corell's insight:

Biomarker: 41-plex Panel Out Now! High-content human immune cell phenotyping based on Chipcytometry Technology

The Human Immunecell Phenotyping Panel (HIPP) comprises: CD3, CD4, CD5, CD8, CD10, CD11b, CD11c, CD14, CD16, CD19, CD20, CD24, CD25, CD27, CD31, CD38, CD45, CD45RA, CD45RO, CD56, CD69, CD71, CD86, CD206, HLA-DR, IgD, IgG, IgA, T-bet, FoxP3, ROR gt, Helios, IL1b, IL8, IL10, IL12, IL17A, IL17F, IFNy, TNFa, pan Cytokeratin

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Rescooped by Alfredo Corell from Immunology Diagnosis
September 8, 2013 11:34 AM
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Check the last diagnostic test updates at IOBA-InmunoLAB (Infectious and immune-mediated ophtalmic disease diagnosis)

Check the last diagnostic test updates at IOBA-InmunoLAB (Infectious and immune-mediated ophtalmic disease diagnosis) | Immunology Diagnosis | Scoop.it
From www.ioba.es - September 11, 2012 7:08 AM

For any questioun, doubt, english documents or enqueries: .

 

Laboratorio de Inmunología (inmunoLAB)
Edificio IOBA, Campus Miguel Delibes
Paseo de Belén 17
47011 Valladolid
E-mail: inmunolab@ioba.med.uva.es
Tel: (+34) 983 423238 / 983 184755


Via Alfredo Corell
Alfredo Corell's insight:

Any sample (biopsies, brush or impresion cytologies, corneal scraping, vitreous or aqueus humour, ....)

DIAGNOSTIC TESTS FOR:
-Ophtalmic infections
-Ophtalmic inflammation
-Ophtalmic diseases genetics,
-Immune-mediated ocular inflammation

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Alfredo Corell's curator insight, December 17, 2012 5:51 PM

Feel free of asking any question or make any suggestion.
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August 13, 2013 2:48 PM
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Introduction to Flow Cytometry and some aplicaciones ! Innova Biosciences

Introduction to Flow Cytometry and some aplicaciones ! Innova Biosciences | Immunology Diagnosis | Scoop.it
From www.innovabiosciences.com - August 13, 2013 2:48 PM
Flow cytometry applications, click for technical information and a full range of flow cytometry (FC) related products. FAST delivery, expert technical support.
Alfredo Corell's insight:

Some tips, protocols and applications of flow cytometry

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July 29, 2013 3:25 PM
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Cell Counting Using the Trypan Blue Exclusion Method

From www.youtube.com - July 29, 2013 3:25 PM
Video tutorial on cell counting using the Trypan Blue Exclusion method from Celsis IVT.
Alfredo Corell's insight:

a short and easy to follow video about cell counting using Trypan Blue staining for death cells

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July 27, 2013 2:34 PM
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Infectious Disease Diagnosis: New Guideline on Lab Tests - Medscape

Infectious Disease Diagnosis: New Guideline on Lab Tests - Medscape | Immunology Diagnosis | Scoop.it
From login.medscape.com - July 15, 2013 9:56 PM
Infectious Disease Diagnosis: New Guideline on Lab Tests Medscape "The quality of the laboratory services must be excellent in order for the ID physician and other physicians to have confidence that the results will lead to an accurate diagnosis or...
Alfredo Corell's insight:

Microbiological diagnoses based on laboratory testing directly affect patient care and outcomes, including hospital infection control, duration of hospitalization, and laboratory efficiency, according to new guidelines from the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology.

The new recommendations, which address appropriate and accurate use of laboratory tests to diagnose infectious diseases (IDs), were published online July 10 in Clinical Infectious Diseases by Ellen Jo Baron, PhD, from the Department of Pathology, Stanford University School of Medicine, and Cepheid, R&D, Sunnyvale, California, and colleagues.

Direct link to the Clinical Infectious Diseases Journal: http://cid.oxfordjournals.org/content/early/2013/06/24/cid.cit278.full

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July 7, 2013 2:12 PM
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Immune monitoring using the predictive power of immune profiles

Immune monitoring using the predictive power of immune profiles | Immunology Diagnosis | Scoop.it
From www.immunotherapyofcancer.org - July 7, 2013 2:12 PM
We have developed a novel approach to categorize immunity in patients that uses a combination of whole blood flow cytometry and hierarchical clustering.
Alfredo Corell's insight:
Conclusions

Comprehensive multi-factorial immune analysis resulting in immune profiles were prognostic, uncovered relationships among immune markers and identified a potential biomarker for the prognosis of cancer. Immune profiles may be useful to streamline evaluation of immune modulating therapies and continue to identify immune based biomarkers.

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October 27, 2013 6:13 PM
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Collaboration of Caltech Engineers and LeukoDX Produces Prototype Point-of-Care Device That Counts White Blood Cells in Minutes in the Patient’s Home

Collaboration of Caltech Engineers and LeukoDX Produces Prototype Point-of-Care Device That Counts White Blood Cells in Minutes in the Patient’s Home | Immunology Diagnosis | Scoop.it
From www.darkdaily.com - October 27, 2013 6:13 PM
Over the last couple of years, medical laboratory technology developers have brought a number of FDA-approved, point-of-care (POCT) and home self-tests to market.
Alfredo Corell's insight:

Sometimes the Quality controls and appropiate use of this devices are not concordant with maufacturer's instructions.

 

Could be useful, but could be dangerous without the proper use.

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October 9, 2013 6:25 PM
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NEW FALL GECLID-SEI COURSES: Transplant Diagnostic Reports and Introduction to aQuality in the Lab - starting 28th october

NEW FALL GECLID-SEI COURSES: Transplant Diagnostic Reports and Introduction to aQuality in the Lab - starting 28th october | Immunology Diagnosis | Scoop.it
From www.geclidsei.uva.es - October 9, 2013 6:25 PM
Programa de Garantía Externa de Calidad para Laboratorios de Inmunología Diagnóstica
Alfredo Corell's insight:

Programm for the Renal transplantation course:

http://www.geclidsei.uva.es/mod/resource/view.php?id=3188

 

Programm for the Introduction to Quality in the Laboratory:

http://www.geclidsei.uva.es/mod/resource/view.php?id=3189 

 

NOW with CFC recognition credits

You cand follow them completely "on-line"

 

Official language: Spanish

 

 

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October 13, 2013 8:56 AM
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Primary Immunodeficiency May Be Misdiagnosed as Cow's Milk ...

Primary Immunodeficiency May Be Misdiagnosed as Cow's Milk ... | Immunology Diagnosis | Scoop.it
From www.hindawi.com - September 30, 2013 11:27 AM
To describe 7 patients referred to a tertiary allergy/immunology Center with a proposed diagnosis of CMA, who were ultimately diagnosed with PID. Methods. This was a retrospective study based on clinical and laboratory data from medical ...
Alfredo Corell's insight:
ISRN Pediatrics
Volume 2013 (2013), Article ID 470286, 6 pages
http://dx.doi.org/10.1155/2013/470286Clinical StudyPrimary Immunodeficiency May Be Misdiagnosed as Cow’s Milk Allergy: Seven Cases Referred to a Tertiary Pediatric HospitalKarina Mescouto Melo,1 Ellen Dantas,1 Maria Isabel De Moraes-Pinto,1 Antonio Condino-Neto,2 Isabela G. S. Gonzalez,1 Marcia C. Mallozi,1 Jackeline M. Franco,3 andBeatriz T. Costa-Carvalho1
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Gilbert C FAURE's curator insight, October 13, 2013 1:23 PM

showing the difficulty of medical diagnosis, as well as the requirement of training either initial or continuous
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October 5, 2013 7:00 PM
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Tideca: Tiempos de cambio en la bioquímica

Tideca: Tiempos de cambio en la bioquímica | Immunology Diagnosis | Scoop.it
From www.tideca.net - October 5, 2013 7:00 PM

TIEMPOS DE CAMBIO EN LA BIOQUÍMICA

Alfredo Corell's insight:

Un lugar difereente para enfocar desde nuevos ángulos la bioquímica y el diagnóstico:

 

excelente colección de casos prácticos

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October 1, 2013 5:59 PM
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Basophil activation testing

Basophil activation testing | Immunology Diagnosis | Scoop.it
From www.jacionline.org - October 1, 2013 5:59 PM
The Journal of Allergy and Clinical Immunology, Volume 132, Issue 4, Pages 777-787, October 2013, Authors:Donald W. MacGlashan, MD, PhD
Alfredo Corell's insight:

Both the treatment of patients with allergic diseases and the study of allergic disease mechanisms depend on a wide variety of assays that in various ways assess the presence and function of IgE antibody. The study of allergic diseases could benefit from the study of its 2 principle cellular participants, mast cells and basophils, but the basophil is more accessible than mast cells for ex vivo studies. Its functionality is tested by using 2 predominant methodologies: the secretion of mediators of allergic inflammation and the expression of proteins on the plasma membrane after stimulation. Each approach has benefits. There are also many operational details to consider regardless of which general approach is taken, and proper interpretation of the methods requires a good understanding of the reagents used and the receptors expressed on basophils and a detailed understanding of the factors regulating aggregation of cell-surface IgE.

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Gilbert C FAURE's curator insight, October 3, 2013 10:17 AM

of course, we lost cytologist's competencies... to look at cells.

We have to rely on secretions assays or cytometric analysis which work pretty well in our experience. Unfortunately, clinical data to correlate biological are not easy to collect and the topic is not very fashionable, somehow outdated.

But, if JACI is devoting a review on the topic, the tests might come back on stage?
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September 29, 2013 11:51 AM
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Clinical Cytometry - Volume 84, Issue 5 - Validation of Cell-Based Fluorescence Assays: Practice Guidelines from the International Council for Standardization of Haematology and t...

Clinical Cytometry - Volume 84, Issue 5 - Validation of Cell-Based Fluorescence Assays: Practice Guidelines from the International Council for Standardization of Haematology and t... | Immunology Diagnosis | Scoop.it
From onlinelibrary.wiley.com - September 29, 2013 11:51 AM
Alfredo Corell's insight:
Cytometry Part B: Clinical CytometrySpecial Issue: Validation of Cell-Based Fluorescence Assays: Practice Guidelines from the International Council for Standardization of Haematology and the International Clinical Cytometry SocietySeptember/October 2013

Volume 84, Issue 5

Pages 279–357

Issue edited by: Marie C. Béné, Gerald E. Marti

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Gilbert C FAURE's curator insight, September 29, 2013 2:22 PM

thanks MC/Bruce et al...
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September 22, 2013 2:13 PM
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Smartphone 'microscope' can detect a single virus, nanoparticles

Smartphone 'microscope' can detect a single virus, nanoparticles | Immunology Diagnosis | Scoop.it
From www.sciencedaily.com - September 22, 2013 2:13 PM
Your smartphone now can see what the naked eye cannot: A single virus and bits of material less than one-thousandth of the width of a human hair.
Alfredo Corell's insight:

Aydogan Ozcan, a professor of electrical engineering and bioengineering at the UCLA Henry Samueli School of Engineering and Applied Science, and his team have created a portable smartphone attachment that can be used to perform sophisticated field testing to detect viruses and bacteria without the need for bulky and expensive microscopes and lab equipment. The device weighs less than half a pound.

 

You can download the Original Paper at ACS Nano in pdf format:

http://pubs.acs.org/journal/ancac3 ;

 

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September 13, 2013 4:00 PM
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Cytometry for immunology: A stable and happy marriage - Cossarizza

Cytometry for immunology: A stable and happy marriage - Cossarizza | Immunology Diagnosis | Scoop.it
From onlinelibrary.wiley.com - September 13, 2013 4:00 PM

Editorial

You have free access to this contentCytometry for immunology: A stable and happy marriageAndrea Cossarizza1,*, Andreas Radbruch2,*

Article first published online: 2 AUG 2013

DOI: 10.1002/cyto.a.22336

Alfredo Corell's insight:

The XV International Congress of Immunology (ICI) held in Milan, Italy, August 22–27, 2013 is likely a perfect occasion to celebrate a marriage that was officiated in the mid-1970s, when monoclonal antibodies (mAbs) were created and became available for research. After a very brief period of engagement, the “official exchange of rings” was represented by the possibility to conjugate mAbs with adequate fluorochromes. This changed almost completely the main use of an instrument, that is, the flow cytometer, that had been created and commercialized few years before for the measurement of cellular DNA by fluorescent intercalating dyes such as ethidium bromide. The recognition of immune cells stained with fluorescent mAbs, typically performed by fluorescence microscopy, was then passed to complex (and at that time quite esoteric) instruments that could analyze thousands of cells in a few seconds, and could provide informative and highly repetitive results.

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Gilbert C FAURE's curator insight, September 14, 2013 2:53 AM

like any marriage, jealousy from other disciplines may occur... although cell imaging and other cytomics approaches should unite more scientists in searching mysteries of the cells, numerous or rare in blood and biological fluids still unexplored
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September 1, 2013 3:00 PM
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Accurate measurement of intact Igs allows improved monitoring of Multiple Myeloma patients.

Accurate measurement of intact Igs allows improved monitoring of Multiple Myeloma patients. | Immunology Diagnosis | Scoop.it
From blog.bindingsite.com - September 1, 2013 3:00 PM
Alfredo Corell's insight:

Free e-book about HeavyLite and FreeLite kits in the use of Free ligth chain and intact immunoglobulins cuantification in the diagnosis and follow up of Multiple Myeloma patients.

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July 31, 2013 5:08 PM
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Se centralizan en el IOBA los análisis de calidad diagnóstica celular de toda España y Portugal

Se centralizan en el IOBA los análisis de calidad diagnóstica celular de toda España y Portugal | Immunology Diagnosis | Scoop.it
From www.rtvcyl.es - July 31, 2013 5:08 PM
Noticias, Vídeos y Guía TV de Cyltv y La Ocho de Castilla y León.
Alfredo Corell's insight:

Tras el convenio firmado con la SEI en 2010, se añade ahora la Sociedad Ibérica de Citometría al programa de garantía de calidad. Se homogeneizaran y certificará la calidad diagnóstica de laboratorios que en el día a día se enfrente a leucemias, trasplantes, SIDA, diabetes (entre otras enfermedades)

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Alfredo Corell's curator insight, August 1, 2013 8:10 AM

Tras el convenio firmado con la SEI en 2010, se añade ahora la Sociedad Ibérica de Citometría al programa de garantía de calidad. Se homogeneizaran y certificará la calidad diagnóstica de laboratorios que en el día a día se enfrente a leucemias, trasplantes, SIDA, diabetes (entre otras enfermedades)
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July 27, 2013 2:38 PM
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The 5 Most Misdiagnosed Diseases - Infections in the 1st position

The 5 Most Misdiagnosed Diseases - Infections in the 1st position | Immunology Diagnosis | Scoop.it
From www.fool.com - July 21, 2013 1:50 PM
The 5 Most Misdiagnosed Diseases
Motley Fool
As investors, it also gives us definable opportunities to take advantage of instances where certain medicines or diagnostics may greatly increase in usage to improve patients' quality of life.
Alfredo Corell's insight:

Where do we go from here?
In addition to increasing the awareness for both patients and physicians around these five commonly misdiagnosed diseases, I believe it opens up the door for advancements in diagnostic and molecular technologies.

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July 18, 2013 5:31 PM
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13th conference on clinical cell analysis and 9th course on clinical cytometry

13th conference on clinical cell analysis and 9th course on clinical cytometry | Immunology Diagnosis | Scoop.it
From www.escca.eu - July 18, 2013 5:31 PM
Alfredo Corell's insight:

ESCCA 2013: INSPIRING AND INNOVATIVE

The European Society for Clinical Cell Analysis (ESCCA) will organise ESCCA 2013 over 5 days in Luxembourg:

the 13th Euroconference on Clinical Cell Analysis (12 - 14 November)the 9th European Course on Clinical Cytometry (15-16 November)

These meetings are of major importance for education and scientific exchange in the fields of basic, translational and clinical applications in cytometry. They offer a unique opportunity to learn, to share information and scientific experience, to seek collaborations and to network - in short, to be involved and to meet old and new friends.

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