I2BC Paris-Saclay

Nikon is presenting its latest confocal microscope to the Paris-Saclay community, equipped with a new detector for very high-resolution images (XY : 100 nm).

From January 30 to February 9, 8 days of demonstrations on the Gif Imaging platform will enable you to test the microscope with your own samples .

Don't hesitate to come and take advantage of this opportunity.

More information: here

Annual workshop of electron microscopy at Imagerie-Gif 17-20 October 2023

Subscriptions are open for the annual workshop of electron microscopy at Imagerie-Gif on 17-20 October 2023. This workshop covers all sample preparation techniques for cell biology in electron microscopy. It mixes theorical courses and large practical sessions. Beginners are welcome.
For more information link or please contact: claire.boulogne@i2bc.paris-saclay.fr

You are looking for an internship, come and meet us on October 15 at I2BC, 1 avenue de la terrasse in Gif sur Yvette.

More information: here

Acquire and reinforce the theoretical basis of light microscopy
Deepen and master the practical use of a wide field and confocal microscope
Understand the complementarities of techniques from conventional microscopy to super-resolution.

Registration deadline September 27th  here

Contact: Romain Le Bars <romain.lebars@i2bc.paris-saclay.fr>

Training was the key-word from 28th to 31th of March at Imagerie-Gif and for the 20 attendees of the annual cytometry workshop and the image analysis course. Attendees learnt the basis and advanced techniques of flow cytometry (https://cnrsformation.cnrs.fr/atelier-cytometrie?mc=Cytom%C3%A9trie%20-) under the supervision of Mickael Bourge, or have explored the marvellous world of image analysis during the training course about ImageJ, with Romain Le Bars.

L'école thématique Ecoclim2022 aura lieu à Orsay du 7 au 10 juin 2022.  Cette école a pour objectif d'offrir une formation de base sur les enjeux environnementaux aux chercheuses et chercheurs de toutes disciplines.

Les renseignements et le formulaire d'inscription sont disponibles sur la page https://indico.ijclab.in2p3.fr/event/7951

Notez qu'un suivi en distanciel sera possible, de préférence pour les personnes en province, mais sans interaction possible. 

Les organisateurs : 

Guillaume Blanc et Guillaume Roux

Au programme

Cours introductif sur le climat

Jean-Yves Grandpeix (LMD)

Dérèglement climatique et impacts

Amaëlle Landais (LSCE)
Didier Paillard (LSCE)
Jeanne Gherardi (LSCE)
Mélanie Becker (LIENSs)

Cycles biogéochimiques, sols et agriculture

Bertrand Guenet (LG-ENS)
Julia Le Noé (ENS)
Petros Chatzimpiros (LIED)

Trajectoires de décarbonation

Henri Waisman (IDDRI)
Gilles Billen (METIS) ( à confirmer)
Aurélien Bigo ()
Emmanuelle Frenoux (LISN)
Tamara Ben Ari (iEES, Labo1point5)

Scénarios et modèles de sociétés

Luc Semal (CESCO)
Timothée Parrique (Lund University)
Emmanuel Combet / Valérie Quiniou / David Marchal (Ademe)

The I2BC NGS facility organizes a new training on the preparation of small RNA libraries with technical tips and tricks to conduct successful small RNA NGS experiments.

MicroRNAs (miRNAs) and other types of small RNA (sRNA) molecules play important roles in the cell and their dysregulation has been linked to various diseases. Indeed, circulating miRNAs are found in various human body fluids and are of interest as potential new non-invasive biomarkers. Next-generation sequencing (NGS) allows a very sensitive and quantitative detection of sRNA expression profiles. However, NGS library preparation from sRNAs is technically relatively challenging as compared to NGS protocols for other applications. Classical sRNA library preparation methods often introduce serious biases leading to sequencing data that do not represent the original sRNA expression profiles. The new training organized by the I2BC NGS facility focusses on technical aspects of NGS library preparation from different types of sRNAs. These include natural sRNAs such as miRNAs, and artificial sRNAs such as ribosome-protected fragments (Ribo-seq) or co-immunoprecipitated sRNA fragments (e.g. CLIP-seq/RIP-seq). Various sRNA library preparation protocols exist and during the training advantages and pitfalls of these methods will be discussed. Specific adaptations required for different types of sRNA will be highlighted. A practical course will teach the participants how to prepare sRNA NGS libraries themselves and how to perform quality control. Together, this training will enable participants to prepare sRNA libraries themselves autonomously, to validate the quality of their libraries and to perform successful NGS experiments by being cautious of technical pitfalls.

More information: https://cnrsformation.cnrs.fr/preparer-des-banques-ngs-a-partir-des-petits-arn-pour-la-technologie-illumina

From October the 3rd to October the 10th

Registration before September the 28th.

More information here

Contact: Claire Boulogne <claire.boulogne@i2bc.paris-saclay.fr>

To acquire the theoretical basis of transmission electron microscopy (TEM) necessary for a functional exploration of the cell
Learn the different sample preparation techniques associated with TEM
Learn how to prepare your own sample

Deadline registration September 27th.

More information: here

METABIODIVEX, in which the I2BC is participating, and the IRN i-NPChem are organising the first thematic school entitled "Molecular Exploration of Biodiversity: New Challenges, New Strategies". It will take place from June 26 to July 1st 2022 in Aussois.

The themes will be :

- Chemodiversity in the era of "Big Data"
- Drug Discovery: Challenges and Strategies
- Evolution and diversity of plant metabolism
- Chemical ecology of marine organisms
- Discovery and production of microbial metabolites: from the gene to the molecule
- Digital chemistry and quantum computing

Registrations until May 15th 2022: https://www.azur-colloque.fr/DR04/inscription

 More information: https://metabiodivex.sciencesconf.org/

Contact: Sylvie LAUTRU <sylvie.lautru@i2bc.paris-saclay.fr>

I2BC teams collaborate and create a teaching project to discover new phages with Bachelor students from Université Paris-Saclay.

Financed by a grant from the University Paris-Saclay to develop teaching innovation, lecturers from I2BC’s Virology and Microbiology Departments have created a new lab course unit, called “Phage Discovery”, for second-year Bachelor students.
In September 2021, thirteen “phage hunters” signed up for this elective course and collected soil and water samples from the campus of Orsay and surroundings. Following purification, they isolated three distinct viruses that infect the bacterium Corynebacterium glutamicum. The students were welcomed by the cryo-electron microscopy platform of I2BC by Ana Arteni, Laura Pieri and Malika Ould Ali. Student phage hunters were dazzled by electron micrographs of their phage samples. Their day at the platform was the highlight of their first research experience.
The project will continue after the sequencing of the viral DNA by the I2BC platform: third-year Bachelor students will annotate the phage genomes. Stay tuned!
Contact: Ombeline Rossier, Christophe Regeard