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Scooped by Gilbert C FAURE
November 16, 2011 12:07 PM
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An improved intracellular staining protocol for efficient detection of nuclear ... - BioTechniques.com

An improved intracellular staining protocol for efficient detection of nuclear ... - BioTechniques.com | from Flow Cytometry to Cytomics | Scoop.it
An improved intracellular staining protocol for efficient detection of nuclear ...BioTechniques.comIntracellular staining is a widely used flow cytometry (FCM)-based technique to detect the expression of cytosolic or nucleic antigens.
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Scooped by Gilbert C FAURE
November 15, 2011 11:03 AM
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Partec to Sell De Novo Software’s FCS Express

Partec to Sell De Novo Software’s FCS Express | from Flow Cytometry to Cytomics | Scoop.it
Partec, a pioneer in flow cytometry reagents and tools, have entered into a deal with De Novo Software, a provider of analysis solutions for flow cytometry data, to sell FCS Express, a flagship product of De Novo Software, to global customers in...
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Scooped by Gilbert C FAURE
November 14, 2011 9:44 AM
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Fluorescent cell barcoding in flow cytometry | Stem Cell Assays

Fluorescent cell barcoding in flow cytometry | Stem Cell Assays | from Flow Cytometry to Cytomics | Scoop.it
Fluorescent cell barcoding (FBC) was developed in Nolan’s lab at Stanford University a few years ago. For the first time, they used it for studying signaling pathways in activated/ inhibited T-cells.
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Scooped by Gilbert C FAURE
November 12, 2011 2:51 PM
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Millipore - Insightful Cellular Analysis

Millipore - Insightful Cellular Analysis | from Flow Cytometry to Cytomics | Scoop.it
Insightful cellular analysis: #cell counting 2 flow #cytometry http://t.co/0394ApyN #SfN11 Booth 1025...
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Scooped by Gilbert C FAURE
November 12, 2011 2:20 AM
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CD200 Expression in B-Cell Chronic Lymphoproliferative Disor... : Journal of Investigative Medicine

Background: Flow cytometry immunophenotyping (FCIP) is used for rapid, specific diagnosis of B-chron (Ahead of Print: CD200 Expression in B-Cell Chronic Lymphoproliferative Disorders: Background: Flow cytometry imm...)...
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Scooped by Gilbert C FAURE
November 11, 2011 9:19 AM
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GEN | News Highlights:FDA Sanctions NYBC’s Cord Blood Hematopoietic Progenitor Cells for Transplantation

GEN | News Highlights:FDA Sanctions NYBC’s Cord Blood Hematopoietic Progenitor Cells for Transplantation | from Flow Cytometry to Cytomics | Scoop.it
World-first approval will allow transplantation of Hemacord allogeneic HPCs for treating acquired and inherited hematopoietic system disorders, as well as those resulting from myeloablative therapy.
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Scooped by Gilbert C FAURE
November 11, 2011 4:24 AM
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DRAQ7™ Novel Cell-Impermeant DNA Dye - SelectScience.net (press release)

DRAQ7™ Novel Cell-Impermeant DNA Dye - SelectScience.net (press release) | from Flow Cytometry to Cytomics | Scoop.it
DRAQ7™ Novel Cell-Impermeant DNA DyeSelectScience.net (press release)DRAQ7™ only labels membrane-compromised dead, dying and apoptotic cells and gives clear discrimination between these and healthy cells in high content cytotoxicity and viability...
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Scooped by Gilbert C FAURE
November 11, 2011 4:16 AM
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Partec to Distribute FCS Express 4 Flow Cytometry with CyFlow Cube™ Instruments

LOS ANGELES, Nov. 10, 2011 /PRNewswire/ -- De Novo Software™, a leading provider of flow cytometry data...
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Scooped by Gilbert C FAURE
November 10, 2011 12:39 PM
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Recent advances in cytometry applications: preclinical, clinical, and ...

The acceptance of flow cytometry (FCM) in clinical laboratory medicine is a major stepping stone towards development new cell analyses, improvement of accuracy, and finally a new range of diagnostic tests.
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Scooped by Gilbert C FAURE
November 9, 2011 8:24 AM
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Post meeting coverage: Roche / Nature Medicine Symposium on Cancer Immunology and Immunotherapy

Nature - the world's best science and medicine on your desktop (RT @natureauthors: MT @NatureConf: Symposium on Cancer Immunology and Immunotherapy- post meeting report, etc.)...
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Scooped by Gilbert C FAURE
November 7, 2011 10:08 AM
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Report on the use of flow cytometry in life science research. | Antibody Chain

In order to dive more deeply into the characteristics and dynamics of the market for flow cytometry, Percepta has introduced the Flow Cytometry Dashboard™, designed to take a snapshot of the current market landscape with the future goal of...
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Scooped by Gilbert C FAURE
November 7, 2011 2:22 AM
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Hematology Textbooks » Flow Cytometry in Neoplastic Hematology, Second Edition: Morphologic–Immunophenotypic Correlation

Hematology Textbooks » Flow Cytometry in Neoplastic Hematology, Second Edition: Morphologic–Immunophenotypic Correlation | from Flow Cytometry to Cytomics | Scoop.it
Hematology Textbooks » Flow Cytometry in Neoplastic Hematology ...
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Scooped by Gilbert C FAURE
November 5, 2011 2:32 PM
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Application of Tesla C2050 to Flow Cytometry and Cancer Detection view file - /view/10_keyword-pipeline-anatomy/application-of-tesla-c2050-to-flow-cytometry-and-cancer-detection.pdf

Free Ebook Application of Tesla C2050 to Flow Cytometry and Cancer Detection view file http://t.co/3Vj57v3F...
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Scooped by Gilbert C FAURE
November 15, 2011 2:58 PM
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GEN | Magazine Articles: Flow Cytometry Shifting Gears

GEN | Magazine Articles: Flow Cytometry Shifting Gears | from Flow Cytometry to Cytomics | Scoop.it
Technology Shedding Its Slow, Cumbersome Reputation and Entering High-Throughput Realm (#Flow Cytometry Shifting Gears http://t.co/MgfzzyDE...)...
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Scooped by Gilbert C FAURE
November 14, 2011 9:45 AM
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TM's 1st world molecular & cell biology online conference - Nature.com (subscription)

TM's 1st world molecular & cell biology online conferenceNature.com (subscription)Attendees participate in molecular & cell biology online conference for free and without travel. Register early to secure your seats.
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Scooped by Gilbert C FAURE
November 13, 2011 2:58 AM
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Potency determination of recombinant IFN-alpha based on phosphorylated STAT1 using flow cytometry

Potency determination of recombinant IFN-alpha based on phosphorylated STAT1 using flow cytometry | from Flow Cytometry to Cytomics | Scoop.it
Potency determination of recombinant IFN-alpha based on phosphorylated STAT1 using flow cytometry - Life Sciences Social Network (Potency determination of recombinant IFN-alpha based on phosphorylated STAT1 using flow cytometry
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Scooped by Gilbert C FAURE
November 12, 2011 10:40 AM
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Amazon.com: Flow Cytometry in Drug Discovery and Development eBook: Virginia Litwin, Philip Marder: Kindle Store

Amazon.com: Flow Cytometry in Drug Discovery and Development eBook: Virginia Litwin, Philip Marder: Kindle Store (Flow Cytometry in Drug Discovery and Development: This book covers the unique application of flow cytometry in d...)...
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Scooped by Gilbert C FAURE
November 11, 2011 9:20 AM
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ECI 2012

ECI 2012 | from Flow Cytometry to Cytomics | Scoop.it
Mark Your Calendar (#ECI2012 - Mark Your Calendar: http://t.co/fCic8cg3 Hope I've got enough together for an abstract!
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Scooped by Gilbert C FAURE
November 11, 2011 9:12 AM
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CD304 is preferentially expressed on a subset of B-lineage acute ...

The quantification of MRD by flow cytometry (FC) is based on the identification of a leukemia-associated phenotype (LAP). Because phenotypic switch is common during treatment, multiple LAPs must be available and used for ...
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Scooped by Gilbert C FAURE
November 11, 2011 4:20 AM
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Download Cyflogic 1.2.1 Flow cytometry data analysis made e... - SoftBuz.Com

Download Cyflogic 1.2.1 Flow cytometry data analysis made e... - SoftBuz.Com | from Flow Cytometry to Cytomics | Scoop.it
Cyflogic 1.2.1 Flow cytometry data analysis made easy.
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Scooped by Gilbert C FAURE
November 10, 2011 12:40 PM
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Cytometry of intracellular signaling: from laboratory bench to clinical ...

The analysis of signaling pathways based on combinations of phospho-specific antibodies is now a well-recognized flow cytometry technique. Despite its wide-ranging potential in the fields of biology, industry, and medicine, ...
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Scooped by Gilbert C FAURE
November 10, 2011 12:32 PM
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Trends in Immunology - E4BP4: an unexpected player in the immune response

E4BP4: an unexpected player in the immune response http://t.co/gA3R9ati...
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Scooped by Gilbert C FAURE
November 8, 2011 5:27 AM
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Author reveals medical mystery woman in 'The Immortal Life of Henrietta Lacks' - The State Journal-Register

Author reveals medical mystery woman in 'The Immortal Life of Henrietta Lacks' - The State Journal-Register | from Flow Cytometry to Cytomics | Scoop.it
La Jolla LightAuthor reveals medical mystery woman in 'The Immortal Life of Henrietta Lacks'The State Journal-RegisterAfter a few confusing minutes lecturing about cellular biology, Defler wrote a name on the chalkboard in large letters: HENRIETTA...
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Scooped by Gilbert C FAURE
November 7, 2011 10:07 AM
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High throughput flow cytometry based yeast two-h... [Cytometry A. 2011] - PubMed - NCBI

PubMed comprises more than 21 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Gilbert C FAURE's insight:
Abstract

The analysis of protein-protein interactions is a key focus of proteomics efforts. The yeast two-hybrid system (Y2H) has been the most commonly used method in genome-wide searches for protein interaction partners. However, the throughput of the current yeast two-hybrid array approach is hampered by the involvement of the time-consuming LacZ assay and/or the incompatibility of liquid handling automation due to the requirement for selection of colonies/diploids on agar plates. To facilitate large-scale Y2H assays, we report a novel array approach by coupling a GFP reporter based Y2H system with high throughput flow cytometry that enables the processing of a 96-well plate in as little as 3 min. In this approach, the yEGFP reporter has been established in both AH109 (MATa) and Y187 (MATα) reporter cells. It not only allows the generation of two copies of GFP reporter genes in diploid cells, but also allows the convenient determination of self-activators generated from both bait and prey constructs by flow cytometry. We demonstrate a Y2H array assay procedure that is carried out completely in liquid media in 96-well plates by mating bait and prey cells in liquid YPD media, selecting the diploids containing positive interaction pairs in selective media and analyzing the GFP reporter directly by flow cytometry. We have evaluated this flow cytometry based array procedure by showing that the interaction of the positive control pair P53/T is able to be reproducibly detected at 72 hr postmating compared with the negative control pairs. We conclude that our flow cytometry based yeast two-hybrid approach is robust, convenient, quantitative, and is amenable to large-scale analysis using liquid-handling automation.

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Scooped by Gilbert C FAURE
November 6, 2011 8:14 AM
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Amazon.com: Flow Cytometry: Principles and Applications eBook: Marion G. Macey: Kindle Store

Amazon.com: Flow Cytometry: Principles and Applications eBook: Marion G.
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