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BigField GEG Tech
June 25, 2014 5:37 AM
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Interesting example of Lentiviral vector use to induce loss of function in the CNS.
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BigField GEG Tech
June 18, 2014 6:29 AM
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The correction of genetic mutations by homologous recombination is an attractive approach to gene therapy. We used the DNA double-strand breaks introduced by the site-specific endonuclease I-Sce1 as a means of increasing homologous recombination of an exogenous DNA template in murine hematopoietic stem cells (mHSCs). To develop this approach, we chose an Artemis knockout (Art−/−) mouse in which exon 12 of the Artemis gene had been replaced by an I-Sce1 recognition site. The I-Sce1 enzyme and the Artemis correction template were each delivered by a self-inactivating (SIN)-integrase-defective lentiviral vector (SIN-IDLV-CMV-ISce1 and SIN-IDLV-Art, respectively). Transduction of Art−/− mHSCs with the two vectors successfully reverted the Art−/− phenotype in 2 of our 10 experiments. Even though the potential for genotoxicity has yet to be evaluated, this new approach to gene editing appears to be promising. Improving the efficacy of this approach will require further technical work.
Nice example of a genome editing tool vectorization in Non Integrating Lentiviral vectors.
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BigField GEG Tech
June 13, 2014 9:34 AM
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Authors described an efficient method for introducing nucleases into cells as proteins packaged within lentiviral vector particles to induce efficient targeted mutagenesis HEK293H and primary T cells. When a repair template sequence was packaged in the lentiviral vector, high levels of homologous gene targeting were obtained and toxicity was markedly reduced.
GEG-tech offers similar type of tools with Lenti-ONE vectors Epi or Trans (RNA) to express Cre, PhiC31 or your favourite recombinase thanks our "on demand service".
http://geg-tech.com/products/vectors.html?applications=Recombinase%2C+editors
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BigField GEG Tech
June 10, 2014 9:06 AM
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Nice example of lentiviral use to express sh RNA in the central nervous system. The endospanin 1 short hairpin RNA expressed by lentiviral vectors allows to decrease endospanin 1 expression by 80% in the N46 hypothalamic cell line and at least by 30% after stereotactic bilateral injection in the ARC of male C57/Bl6 mice.
GEG-tech offers sh-RNA lentiviral vectors for in vitro and in vivo use. If you wish, you can see our video about it to know more...
http://geg-tech.com/rna-interference.html
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BigField GEG Tech
June 4, 2014 11:55 AM
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In this new study, the use of lentiviral vector which expresses the Cre recombinase allows a new non-invasive approach and specific monitoring of eNSCs over time, opening perspectives for preclinical evaluation of candidate stroke therapeutics.
GEG-tech offer this type of vector which is declined in integrative, non integrative and RNA vectors. The last one allows a full transient expression which is generally very usefull to express recombinases.
http://geg-tech.com/products/vectors.html?transgene=Cre-NLS
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BigField GEG Tech
May 5, 2014 5:50 AM
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The American Journal of Pathology, Volume 183, Issue 3, Pages 964-974, September 2013, Authors:Haibo Wang; George W. Smith; Zhihong Yang; Yanchao Jiang; Manabu McCloskey; Kenneth Greenberg; Pete Geisen; William D. Culp; John Flannery; Tal Kafri; Scott Hammond; M. Elizabeth Hartnett
An example of Sh lentivector to knockdown the VEGF. GEG-tech offers all types of sh Lentivector through the "on demand" service. Furthermore a whole collection of Lentivectors VEGF165b, which is an antagonist of the VEGF, is available in our web site.
http://geg-tech.com/products/vectors.html?env=VSV&transgene=VEGF165b
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BigField GEG Tech
April 29, 2014 11:18 AM
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GEG-tech offers similar vectors as those used by Yáñez-Muñoz et. al. in this study. Furthermore, GEG-tech allows you to choose several types of IDLV (different intergase mutations) or promoters and offers the mokola envelope which is specific of astrocytes.
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BigField GEG Tech
April 15, 2014 6:19 AM
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Proof-of-principle of CRISPR-Cas9 system to correct disease genes in vivo in adult mouse liver. The authors described a substantial improvement of efficiency to correct hemophilia in mice compared to the initial efficiency of repair using zinc finger nucleases.
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BigField GEG Tech
April 11, 2014 4:05 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Nice example of tool design for gene transfer. The authors have tested several promoters in the piggyback transposon system to determine the best configuration in their experimental context. Exactly that GEG-tech offers for lentiviral vectors through the different set of vectors to select the promoter or other features the most adapted to your study.
http://geg-tech.com/products/sets.html
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BigField GEG Tech
April 4, 2014 6:59 AM
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Illustration of Non Integrating Lentiviral Vectors efficiency in central nervous system.This is typically the type of vector available on GEG-tech website with very high titer.
http://geg-tech.com/products/vectors/v020008001.html
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BigField GEG Tech
April 3, 2014 9:19 AM
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New strategy for iPS base on dual integrase cassette exchange (DICE) mediated by phiC31 and Bxb1 integrases into a safe locus.
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BigField GEG Tech
March 25, 2014 6:12 AM
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Non integrating lentiviral vectors allow long term expression in neuron with a level of expression which could be compatible with some therapeutic strategies.
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BigField GEG Tech
from Animal Models - GEG Tech top picks
July 4, 2014 8:14 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Lentiviral vectors which express shRNA to study cardiac features.
Lentiviral vectors which express shRNA to study cardiac features.
http://geg-tech.com/rna-interference.html
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BigField GEG Tech
June 24, 2014 6:18 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
New scientific publication of GEG-tech.
Proof of concept of the PhiC31 recombinase vectorization in a Non Integrating Lentiviral Vector system, a promising tool to mediate genome editing. This article shows the potentiel of this tools for gene transfer applications as well as the genotoxic effects which can occurs in some cases. This article suggests several ways of improvement to avoid these undesirable events.
GEG-tech offers this type of vectors, substrat and recombinase :
http://geg-tech.com/products/vectors.html?applications=Recombinase%2C+editors
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BigField GEG Tech
June 18, 2014 6:00 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
Promising results about the use of Non Integrating Lentiviral Vectors for vaccination.
GEG-tech offers the largest range of Non Integrating Lentiviral Vectors. Different integrase mutations are available and you have the possibility to select the NILVs the most adapted for your research thanks to our "set of vectors".
http://geg-tech.com/products/sets/integrase-set-starter.html
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BigField GEG Tech
June 10, 2014 9:52 AM
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The first strategy which allows to deliver TALENs from a lentiviral vector. This is possible because the TAL-effector nucleases is fused to Gag precursors and delivered directly as a protein.
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BigField GEG Tech
June 6, 2014 10:53 AM
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Interesting combination between zinc finger nucleases and integrase-defective lentiviral vectors to demonstrate for the first time that FANCA can be efficiently and specifically targeted into the AAVS1 safe harbor locus in fibroblasts from FA-A patients. Up to 40% of FA fibroblasts showed gene targeting 42 days after gene editing.
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BigField GEG Tech
May 12, 2014 6:40 AM
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PubMed comprises more than 23 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In this study, the authors used lentiviral vectors to generate transgenic chickens that express the human EC-SOD gene. They obtained 1/16 positive bird and a postive G1 for which hEC-SOD protein was expressed in the egg white and showed antioxidant activity.
GEG-tech provides a large range of vectors for transgenesis with very high titer whose lentiviral vectors which express SOD or other antioxidant proteins.
http://geg-tech.com/products/vectors.html?applications=Apoptosis%2C+oxidative+stress
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BigField GEG Tech
May 2, 2014 3:33 AM
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A gene transfer tool at the service of oncology field. Since two years, Sleeping Beauty allowed to identify loci that participate in tumor initiation, progression and metastasis for human pancreatic, hepatocellular, colorectal and neurological cancers . Oncogenomic approaches have directly linked hundreds of genes identified by SB with human cancers, many with prognostic implications.
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BigField GEG Tech
April 28, 2014 8:06 AM
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Molecular Therapy — Methods & Clinical Development, Published online: 9 April 2014; | doi:10.1038/mtm.2014.10
The lentivirus with the Csf1r element is capable of directing macrophage-restricted reporter gene expression in mouse, rat, human, pig, cow, sheep, and even chicken. Rat bone marrow cells transduced with the lentivirus were capable of differentiating into macrophages expressing the reporter gene in vitro.
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BigField GEG Tech
April 14, 2014 6:36 AM
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This study reports for the first time the generation of keratinocyte-derived iPSCs from a domestic species. Compared with previous reports, equine iPSCs were generated with high efficiency and, more importantly, could be maintained using simplified, feeder-free culture conditions.This work will surely has an impact in veterinary and therapeutic fields.
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BigField GEG Tech
April 8, 2014 6:22 AM
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
The UshStat, an EIAV-based lentiviral gene therapy vector harboring a full length human myosin VIIa expression cassette under the control of a constitutive CMV promoter, restores the α-transducin translocation threshold and reduces the light-dependent photoreceptor degeneration phenotypes in shaker1 mice. These data provided support for the initiation of the first-in-man clinical trial of UshStat in patients with Usher syndrome 1B (ClinicalTrials.gov identifier: NCT01505062) that is currently underway.
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BigField GEG Tech
March 31, 2014 7:34 AM
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Non integrating lentiviral vectors with mini MAR generating mitotically stable episomes capable of long-term transgene expression. 13-45% of cells expressing the transgene for over 100 cell generations in the absence of selection, great!
http://geg-tech.com/