SynBioFromLeukipposInstitute

Population level control - multicellular systems: an interesting area!

Synthetic Biology offers qualitatively new perspectives on the benefits of industrially harnessed biological processes. The ability to modify and reprogramme natural biology increases the scope of tailored bioprocesses and yields attractive prospects beyond conventional Biotechnology. The present review summarises the major achievements and categorises them according to a hierarchy of system levels. Similar structures are known in the engineering sciences and might prove useful for the future development of Synthetic Biology.

Engineering at the scale of whole genomes requires fundamentally new molecular biology tools. Recent advances in recombineering using synthetic oligonucleotides enable the rapid generation of mutants at high efficiency and specificity and can be implemented at the genome scale. With these techniques, libraries of mutants can be generated, from which individuals with functionally useful phenotypes can be isolated. Furthermore, populations of cells can be evolved in situ by directed evolution using complex pools of oligonucleotides. Here, we discuss ways to utilize these multiplexed genome engineering methods, with special emphasis on experimental design and implementation.

"Remote control of cells is a desirable feature in synthetic biology. We established a light-switchable interfering peptide (iPEP) which controls gene expression by modulating the activity of a transcription factor...."

Guest editors Cameron Alexander and Rachel O'Reilly introduce this themed issue of Journal of Materials Chemistry on materials chemistry in the emerging field of synthetic biology.

Scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, have developed a new method which enables researchers to label any protein of their choice with any of a wide variety of previously available compounds, in living cells, by introducing a single reactive artificial amino acid.