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This study from Italy initiated in 1994 looks at the long term effects of CT and NT initially on Durum wheat but added Vicia faba cover crop in 2009 in half of each tillage treatment. They monitored microbial biomass, soluble N, and enzyme activities (EA). Between 2009 and 2018, CT yields were on average 15% higher than NT, especially during high rainfall years, but NT increased SOC in the 0-30 cm soil depth and higher levels of soluble N, dsDNA, and EAs at 0-10 cm depth. Introducing cover crops over 4 years did not yield significant impacts.They conclude that adopting NT practices resulted in higher SOC concentration, enhanced soil biological activity, and improved biogeochemical cycles, emphasizing the positive impact of NT on soil health and sustainability.