Follow, research and publish the best content
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Already have an account: Login
Vectorology - GEG Tech top picks
22.3K views |
+5 today
 Your new post is loading...
Your new post is loading...
Scoop.it!
BigField GEG Tech's insight:
Nice example of lentiviral use to express sh RNA in the central nervous system. The endospanin 1 short hairpin RNA expressed by lentiviral vectors allows to decrease endospanin 1 expression by 80% in the N46 hypothalamic cell line and at least by 30% after stereotactic bilateral injection in the ARC of male C57/Bl6 mice.
http://geg-tech.com/rna-interference.html
|
Scoop.it!
BigField GEG Tech's insight:
The authors showed that pharmacological stimulation of A2A receptors or injection of lentiviral vectors expressing the A2A receptor into white fat induces brown-like cells. Importantly, mice fed a high-fat diet and treated with an A2A agonist are leaner with improved glucose tolerance. These results demonstrate that adenosine–A2A signalling plays an unexpected physiological role in sympathetic BAT activation and protects mice from diet-induced obesity. Those findings reveal new possibilities for developing novel obesity therapies.
|
In this study, the authors injected a lentiviral to overexpress the Neuropeptide Y (NPY) into the paraventricular nucleus (PVN) of rats fed a high fat (HFD) or low-fat diet. 8 weeks later, in vivo intravenous glucose tolerance tests and euglycemic-hyperinsulinemic clamp revealed that insulin resistance of adipose tissue were induced by NPY overexpression with or without HFD. NPY increased food intake, but did not change blood glucose, glycated hemoglobin A1c (HbA1c) or lipid levels.
www.geg-tech.com/Vectors