Duchenne Muscular Dystrophy (DMD) is caused by a lack of dystrophin expression in patient muscle fibres.
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In this work, scientists have demonstrated that lentiviral vectors can package and deliver inserts of a similar size to dystrophin. They report a novel approach for delivering large transgenes in lentiviruses, in which they demonstrate proof-of-concept for a ‘template-switching’ lentiviral vector that harnesses recombination events during reverse-transcription.