The authors compared six HIV vectors carrying different integrases, either wild type or with different mutations shown to modify integrase enzymatic activity, oligomerization, or interaction with key cellular cofactor of HIV DNA integration as LEDGF/p75 or TNPO3. They report that an integrase carrying the D167H substitution improves vector transduction efficiency and integration in both HEK-293T and primary CD34+ cells.
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The authors compared six HIV vectors carrying different integrases, either wild type or with different mutations shown to modify integrase enzymatic activity, oligomerization, or interaction with key cellular cofactor of HIV DNA integration as LEDGF/p75 or TNPO3. They report that an integrase carrying the D167H substitution improves vector transduction efficiency and integration in both HEK-293T and primary CD34+ cells.
http://www.geg-tech.com/Vectors/index.php/line-lenti-one-epi.html