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Animal Models - GEG Tech top picks
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In this study, the scientists present a highly efficient method for introducing gene modifications at almost all target sequences in randomly selected embryos. The gene modification activity of TALEN is enhanced by its fusion to the 3′UTR of the Xenopus DEADSouth gene. The addition of this sequence promotes mRNA translation in the oocytes and increases the expression of TALEN proteins to near-maximal levels three hours post fertilization. 
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Tested using Xenopus tropicalis, the scientists show that founders containing transplants transmit mutant alleles with high efficiency. F1 offspring from intercrosses between F0 that carry embryonic lethal alleles recapitulate loss-of-function phenotypes, circumventing an entire generation of breeding. They anticipate leapfrogging will be transferable to other species.