Animal Models - GEG Tech top picks

This study was conducted to confirm that 1-site and 4-site ppU6-GGTA1-gRNA CRISPR vectors together with the pCX-Flag2-NLS1-Cas9-NLS2 plasmid can both generate KO pigs by direct pronuclear microinjection. In total, 41 and 53 fertilized eggs were microinjected on 1-site and 4-site strategies, respectively.  The results show that mutant pigs could be generated by direct pronuclear microinjection of CRISPR vectors and that the 4-site strategy has a better mutant efficiency.