Animal Models - GEG Tech top picks

Here, the scientists report the first application of single Cas9 nickase (Cas9n) to induce gene insertion at a selected locus in cattle. They demonstrate that a single Cas9n-induced single-strand break can stimulate the insertion of the natural resistance-associated macrophage protein-1 (NRAMP1) gene with reduced, but still considerable, off-target effects. Through somatic cell nuclear transfer, they finally obtain transgenic cattle with increased resistance to tuberculosis. These results contribute to the development of CRISPR-Cas9 system for agriculture applications.