I2BC Paris-Saclay

Unveiling how bacteria modify the surface of solid walls in their close environment at the nanoscale is an important step in nanotechnology and bioengineering as well as in geomicrobiology and fundamental surface chemistry/physics.

Bacteria have evolved on earth for billions of years and have managed to colonize all ecological niches. Their metabolic diversity is so vast that they play a major role in the biogeochemical cycles of our planet. Understanding the mechanisms by which bacteria interact with the first interfacial layers of the solid matter that surrounds them is an important issue with many perspectives in both fundamental and applied science. Thin iron films, with a surface area of centimeters and a thickness of nanometers, have been produced. The nanofilm’s opacity being related to its thickness, it is then possible to measure both the nanofilm’s degradation and to localize and quantify the bacteria dynamics in situ and in real time by simple optical means (backgroud of the picture). This experimental work published in the journal ACS Central Science indicates a homogeneous corrosion of iron nanofilms triggered suddenly by bacteria. Experiments reveal in particular rapid motions of Shewanella oneidensis MR1 bacteria during iron dissolution. Mutants of S. oneidensis as well as other bacterial species such as E. coli and L. plantarum are also able to induce corrosion (figure). All the experiments highlight more generally the role of electroactive respiratory proteins and soluble secreted molecules in the modification of the surface properties of nanofilms.

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Contact person: Christophe Regeard

The GO Paris-Saclay 2021 team won a gold medal and the "Best Inclusivity" prize at the iGEM 2021 international synthetic biology competition on Sunday, November 14.

The team's project "EndoSeek" aims to develop a new diagnostic tool for endometriosis, which is a painful and poorly understood pathology caused by the proliferation of uterine cells outside the uterus. Worldwide, this disease affects about 10% of women and can take up to 7 years to diagnose. Based on preliminary studies using small patient cohorts, certain blood microRNAs (miRNAs) may be biomarkers of the disease.
The team has developed a machine learning program that will allow identification of new endometriosis biomarkers with future cohorts. In experiments conducted this summer at I2BC, students exploited Cas13a and Cas14a1 nucleases for miRNA detection. They created a video game to educate adults and children over the age of 10 about endometriosis and synthetic biology. Finally, following their dialogue with patients and physicians, they questioned the ethical implications of diagnostics.
The team was awarded the Best Inclusivity Award for outstanding efforts to include people with diverse identities. The students thought about the position of LGBT+ people in their project and created a multi-language, voice-assisted website with color customization.
This project was supported by the I2BC, the Faculty of Sciences of the University of Paris-Saclay, La Diagonale Paris-Saclay, EUGLOH (European University Alliance for Global Health), the Graduate School Life Sciences and Health, IDT and Promega.

Team 2021 supervisors included Téo Hébra (ICSN) and 5 members of the I2BC: Philippe Bouloc, Stéphanie Bury-Moné, Emma Piattelli, Ombeline Rossier and Charlène Valadon.
For more information, see the wiki https://2021.igem.org/Team:GO_Paris-Saclay and the video https://video.igem.org/w/ihqYR3UfveimEtZVv7x6jE.

This is the first published evidence linking lipopolysaccharide structural moiety to neonatal sepsis outcome and opens the possibility of using the 2-hydroxymyristate marker as a detection tool for high-risk patients, which could help reduce their mortality.

Enterobacter cloacae complex species are involved in infections among critically ill patients. After a recent E.cloacae outbreak of fulminant neonatal septic shock, we conducted a study to determine whether septic shock severity and its lethal consequence are related to structural features of the endotoxin (lipopolysaccharide [LPS]) of the strains isolated from hospitalized infants and more specifically its lipid A region. It appeared that the LPSs are very heterogeneous, carrying fifteen different molecular species of lipid A. The virulence was correlated with a structural feature identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry and gas chromatography coupled with mass spectrometry: the presence of 2-hydroxymyristic acid as a secondary substituent in lipid A. This is the first published evidence linking LPS structural moiety to neonatal sepsis outcome and opens the possibility of using this fatty acid marker as a detection tool for high-risk patients, which could help reduce their mortality.

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Contact person: Pierre Tissières

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First experimental evidence for type I-B CRISPR-Cas system adaptation in the emerging human enteropathogen C. difficile and a functional link between the adaptation and interference CRISPR machineries sharing similar tri-nucleotide PAM motif for recognition of foreign nucleic acid sequences.

CRISPR-Cas systems provide prokaryotes with adaptive immunity for defense against foreign nucleic acid invaders, such as viruses or phages and plasmids. The CRISPR-Cas systems are highly diverse, and detailed studies of individual CRISPR-Cas subtypes are important for our understanding of various aspects of microbial adaptation strategies and for the potential applications. The significance of this collaborative work of French, Russian and US labs from I2BC, Skoltech and Waksman Institute of Microbiology is in providing the first experimental evidence for type I-B CRISPR-Cas system adaptation in the emerging human enteropathogen Clostridioides difficile. This bacterium needs to survive in phage-rich gut communities, and its active CRISPR-Cas system might provide efficient antiphage defense by acquiring new spacers within CRISPR arrays that constitute memory for further invader elimination. This study also reveals a functional link between the adaptation and interference CRISPR machineries. The definition of all possible functional trinucleotide motifs upstream protospacers within foreign nucleic acid sequences is important for CRISPR-based genome editing in this pathogen and for developing new drugs against C. difficile infections.

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Contact person: Olga Soutourina

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Escherichia coli acquires resistance to polymyxin B upon the activation of two-component systems, but this is at the expense of innate bile acid resistance. The lack of lipopolysaccharide phosphorylation accounts for bile susceptibility and impairs intestinal colonization in mice.

The surface properties of bacterial cells play a key role in resistance to various antimicrobial agents. Gram-negative bacteria expose lipopolysaccharides (LPS) on their surface, which can undergo different structural modifications. However, some of these modifications may be beneficial in some circumstances, but detrimental in others.

In this study, published in Frontiers in Microbiology, the team of Thierry Touzé, in collaboration with a team from Institut Pasteur, showed that Escherichia coli cells resist to cationic antimicrobial peptide polymyxin B, one last resort antibiotic, under conditions that simultaneously activate two-component regulatory systems PmrA/B and PhoP/Q. Among a set of modifications, they identified those responsible for this resistance, which occurs on the lipid A moiety (known as the endotoxin) from LPS. The acquisition of polymyxin B resistance came at the expense of loss of innate resistance to deoxycholate, a major component of bile. They provide evidences that bile susceptibility arises from the inhibition of LpxT-dependent modification, which consists in lipid A phosphorylation. Bile acids are abundant in the small intestine, where they modulate the commensal flora and the researchers further showed that the inactivation of lpxT impaired gut colonization in mice.

These results highlight the importance of lipid A decorations and their tight regulation in the lifestyle of E. coli and probably other Enterobacteriaceae that exhibit the same modifications

https://www.frontiersin.org/articles/10.3389/fmicb.2021.676596/full

Contact: thierry.touze@i2bc.paris-saclay.fr

The transcriptional regulation of the biosynthesis and resistance to netropsin is not only controlled by a transcriptional regulator, Cgc1, but also by netropsin itself, which induces the expression of the resistance genes and of cgc1 by a new type of feedforward induction mechanism.

Understanding the mechanisms of regulation of specialized metabolite production can have important implications both at the level of specialized metabolism study (expression of silent gene clusters) and at the biotechnological level (increase of the production of a metabolite of interest). We report here the first study on the regulation of the biosynthesis of a metabolite from the pyrrolamide family, congocidine (netropsin). Most pyrrolamides bind into the minor groove of DNA, specifically in A/T-rich regions, which gives them numerous biological activities, such as antimicrobial and antitumoral activities. We demonstrate that Cgc1, a cluster-situated, atypical orphan response regulator, controls congocidine biosynthesis and resistance. In addition, we show that congocidine induces the expression of the operon containing the resistance genes, offering protection against congocidine to cells that are not yet producing it. Finally, our results reveal that congocidine induces its own biosynthesis through the induction of the transcription of cgc1, in a new type of feed-forward induction mechanism.

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Contact person: Sylvie Lautru

High throughput analysis of fitness genes in a bacterial pathogen: towards the discovery of new targets to develop treatments.

The pathogenic bacterium Agrobacterium tumefaciens provokes crown-gall disease on a wide diversity of host plants. It colonizes the galls it causes on host plants, poplar and tomato plant for instance. This pathogen also colonizes the roots of host plants and non-host plants, such as maize. We used a genome-wide approach (transposon sequencing) to discover Agrobacterium tumefaciens genes involved in reproductive success on tomato and poplar galls and tomato and maize roots. We used this knowledge to develop plant protection approaches against this pathogen. This wok was supported by the I2BC sequencing platform.

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Contact person: Denis Faure

Last call for registration at the Environmental and Agronomical Genomics 2021 Symposium until 13th October.

The Environmental and Agronomical Genomics 2021 symposium will be the opportunity to have an update on most recent environmental genomics research, as well as an overview of high scientific impact projects carried out in collaboration with France Genomique platforms. This year we are also celebrating the 10th anniversary of the GDR Génomique environnementale.

Contact person: Denis Faure

A joint collaborative effort of the Microbiology, Genome Biology Departments and the Sequencing Facility of the I2BC unveiled the dynamics of the linear chromosome of Streptomyces ambofaciens, during metabolic differentiation.

Streptomyces are soil bacteria mostly known for their complex life cycle (uni- to multi-cellular transition, sporulation, metabolic differentiation) and their prolific specialized metabolism (e.g. antibiotics and pigments), widely exploited in medicine, agriculture and the food industry. Their genome possesses remarkable characteristics including a large size (6-12 Mb) and a high GC content (circa 72%) and, even more unusual in bacteria, a linear configuration. Interestingly, Streptomyces chromosomes present a remarkable genetic compartmentalization, with a distinguishable central region harboring core genes and two terminal regions enriched in specialized metabolite biosynthetic gene clusters. The molecular mechanisms governing the structure and function of these compartmentalized chromosomes remain mostly unknown. In this work, teams of the Genome Biology, the Microbiology Department and the Sequencing Facility of the I2BC in collaboration with the DynAMIC (Lorraine University -INRAe) and the TIMC-IMAG ( Grenoble Alpes University - CNRS) Institutes, show that chromosome structure in Streptomyces ambofaciens correlates with genetic compartmentalization during exponential phase. Conserved, large and highly transcribed genes form boundaries that segment the central part of the chromosome into domains, whereas the terminal ends tend to be transcriptionally quiescent compartments with different structural features. The onset of metabolic differentiation is accompanied by a rearrangement of the chromosome architecture, from a rather ‘open’ to a ‘closed’ conformation, in which highly expressed specialized metabolite biosynthetic genes form new domain boundaries. Altogether, these results indicate that the linear chromosome of S. ambofaciens is partitioned into structurally distinct entities, suggesting a link between chromosome folding, gene expression and genome evolution.

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Contact persons: Virginia Lioy & Stephanie Bury-Mone

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Multiomic analysis of a suboptimal symbiotic interaction where a soybean nodulator (Bradyrhizobium diazoefficiens) faces NCR peptides in Aeschynomene afraspera and undergoes atypical terminal bacteroid differentiation

In the legume-rhizobium nitrogen-fixing symbiosis, some host plants produce antimicrobial peptides called NCR to control their bacterial partner and trigger its cellular differentiation. Consequently, intracellular bacteroids become polyploid, elongated and with a permeabilized membrane, collectively resulting in an altered bacterial viability. We call this phenomenon terminal bacteroid differentiation (TBD), which is associated to a higher return on investment to the host of the symbiotic process.

In the study by Nicoud et al. published in mSystems, we analyzed the physiology of Bradyrhizobium diazoefficiens USDA110 in symbiosis with its natural host soybean, which does not trigger TBD, and in interaction with Aeschynomene afraspera, a host producing NCR peptides and inducing TBD. To do so, we combined whole-nodule metabolomics, with bacterial transcriptomics and proteomics. We found that the maladapted symbiosis between Bradyrhizobium and Aeschynomene is suboptimal (reduced benefit to the host) and that bacteria undergo an intense stress. Finally, we characterized bacteroid differentiation by a combination of flow cytometry and image based morphometry and found a disconnection of the canonical features of TBD, with bacteroids that fail to elongate and endoreduplicate, while their membranes become preamble and their viability reduced.

https://doi.org/10.1128/mSystems.01237-20

Contact: benoit.alunni@i2bc.paris-saclay.fr