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Topical news snippets about viruses that affect people.  And other things. Like Led Zeppelin. And zombies B-)
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Detection of specific HPV subtypes responsible for the pathogenesis of genital warts

Detection of specific HPV subtypes responsible for the pathogenesis of genital warts | Virology News | Scoop.it

The aims of the study were to evaluate current strategies used for the monitoring of HPV infection in genital warts and to assess the suitability of laser-capture microdissection (LCM) as a technique to improve the understanding of the natural history of HPV types associated with genital wart lesions.

Methods

DNA and RNA were extracted from whole wart, surface swabs and LCM sections from 23 patients. HPV types present were determined using the Linear Array HPV Genotyping Test (Roche), with HPV DNA viral load and mRNA expression investigated using qPCR and qRT-PCR, respectively.

Results

Results indicated that swabbing the surface of warts does not accurately reflect potential causative HPV types present within a wart lesion, multiple HPV types being present on the surface of the wart that are absent in the lower layers of tissue isolated by LCM. Although it was shown that HPV DNA viral load does not directly correlate with HPV mRNA load, the presence of both DNA and mRNA from a single HPV type suggested a causative role in lesion development in 8/12 (66.6%) of patients analysed, with dual infections seen in 4/12 (33.3%) cases. HPV 6 and HPV 11 were present in more than 90% of the lesions examined.


HPV graphic courtesy of Russell Kightley Media

Ed Rybicki's insight:

Interesting stuff: so, simply swabbing the surface of lesions does NOT give a good indication as to what is causing the lesion - and HPVs 6 and 11 are often present together in condylomas.

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Multi-virus detector chip created by Singapore team

Multi-virus detector chip created by Singapore team | Virology News | Scoop.it
In a world first, a Singapore scientist and his team have developed a chip which can detect up to 70,000 different viruses and bacteria in one test.
Ed Rybicki's insight:

Virus on a chip: one better than gannet on a stick....

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