Follow, research and publish the best content
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Already have an account: Login
Animal Models - GEG Tech top picks
7.3K views |
+0 today
 Your new post is loading...
Your new post is loading...
Scoop.it!
BigField GEG Tech's insight:
In this study, the authors used sleeping beauty for a site-specific gene knock-in in somatic cell lines which carried a Cre-loxP-cassette exchange of the tagging transposon . Then, with clonal population, they perform somatic cell nuclear transfer to generate a syngeneic cohort of piglets Potentially, the syngeneic cohort of pigs will be instrumental for vital tracking of transplanted cells in pre-clinical assessments of novel cell therapies. 
Scoop.it!
BigField GEG Tech's insight:
Recently, the use of a simple and inexpensive transgenic approach which is a hydrodynamics-based transfection (HT) method coupled with the Sleeping Beauty transposase system has been developed. In this review, the applicability of HT models in liver cancer research is expected to broaden and ultimately elucidate the cooperation between oncogenic signaling pathways and aid in designing molecular therapy to target altered pathways.
|
Scoop.it!
BigField GEG Tech's insight:
In this work, piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transposition is a feasible approach for genetic engineering in the cattle genome.
Scoop.it!
BigField GEG Tech's insight:
Here, the authors describe a simplified microinjection protocol for efficient germline transgenesis and sustained transgene expression in the mouse model employing binary Sleeping Beauty transposon constructs of different topology. The protocol is based on co-injection of supercoiled plasmids or minicircles, encoding the Sleeping Beauty transposase and a transposon construct, into the cytoplasm of murine zygotes.
Scoop.it!
BigField GEG Tech's insight:
The authors have designed cytoplasmic injection assays to generate transgenic pigs thanks to the piggybac transoposase. More than 8.00% of the injected embryos developed into transgenic animals containing monogenic and often single transgenes in their genome.
|
Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, the authors established a highly efficient one-step multiplex gene transfer system into the bovine genome.