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Animal Models - GEG Tech top picks
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BigField GEG Tech's insight:
The adult newt has the ability to regenerate the neural retina following injury, a process achieved primarily by the retinal pigment epithelium (RPE). Thanks to transgenic newts, the authors identified a 340 bp CpG island located between −635 and −296 bp in the promoter which contains response elements for the microphthalmia-associated transcription factor known as MITF (CACGTG, CATGTG), and E-boxes (CANNTG). 
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Conditional gene knockout technology is a powerful tool to study the function of a gene in a specific tissue, organ or cell lineage.
In this study on the deletion of a same gene in two different tissues, the authors show that the incidence rate of non-specific recombination in unwanted tissues depends on the Cre driver strain, ranging from 100 %, rendering it useless (aP2-Cre strain), to ~5 %, which is still compatible with their use (RIP-Cre strain). They conclude that, when choosing the Cre-driver promoter, researchers not only have to be careful about its tissue-specificity and timing of activation, but should also include a systematical screening in order to exclude mice in which atypical recombination has occurred and to limit the unnecessary use of laboratory animals in uninterpretable experiments.
www.geg-tech.com/Vectors