Vitrification is a powerful tool for the efficient production of offspring derived from cryopreserved oocytes or embryos in mammalian species including domestic animals. Although the authors reported the successful production of piglets derived from vitrified PN embryos by a solid-surface vitrification method with glutathione supplementation, further improvements are required and exposed in this article. The findings of this study demonstrate for the first time that carboxylated ε-poly-L-lysine is a promising cryoprotective agent for further improvements in the vitrification of oocytes and embryos in mammalian species.
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