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Animal Models - GEG Tech top picks
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BigField GEG Tech's insight:
The scientists injected zinc finger nuclease (ZFN)-encoding mRNA or DNA into bovine zygotesto target beta-lactoglobulin gene (LGB) and detected ZFN-induced random mutations in 30% to 80% of embryos. Next, to precisely change the LGB sequence, they co-injected ZFNs or transcription activator-like effector nucleases (TALENs) with DNA oligonucleotides (ODNs). Analysis of co-injected embryos showed targeted changes in up to 33% (ZFNs) and 46% (TALENs) of blastocysts. 
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BigField GEG Tech's insight:
In this study, the authors designed a knock-in vector system based on CRISPR for expression of human fibroblast growth factor 2 (FGF2) in the bovine β-casein gene locus. They transfected bovine ear fibroblasts and produced knock-in fibroblasts with a highest efficiency than with conventional methods. In addition, they produced knock-in blastocysts by somatic cell nuclear transfer. In this way they created cloned embryos for development of transgenic dairy cattle expressing human FGF2 protein in the mammary gland via the expression system of the bovine β-casein gene.
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
BigField GEG Tech's insight:
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In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes.
The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest.