Animal Models - GEG Tech top picks

Liao et al. transfected human cell lines with Cas9 and with gRNAs targeting the long terminal repeats (LTRs) of HIV-1, which led to reduced infection of these cells by HIV-1, compared with wild-type cells. Similarly, CRISPR–Cas9 transfection into cell lines with integrated virus reduced the levels of proviral DNA. Furthermore, human T cell lines engineered to stably express Cas9 and LTR-specific gRNAs maintained a low level of viral infection even 14 days post-infection with HIV-1. Finally, the authors showed that CRISPR–Cas9 efficiently reduced virus production in primary human cells, demonstrating that expression of the CRISPR–Cas9 system in humans confers protection against HIV-1 infection.

www.geg-tech.com/Vectors