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High-dimensional, single-cell characterization of the brain's immune compartment Ben Korin, Tamar L Ben-Shaanan, Maya Schiller, Tania Dubovik, Hilla Azulay-Debby, Nadia T Boshnak, Tamar Koren & Asya Rolls AffiliationsContributionsCorresponding author Nature Neuroscience (2017) doi:10.1038/nn.4610 
Gilbert C FAURE's curator insight,
July 25, 2017 4:19 AM
The brain and its borders create a highly dynamic microenvironment populated with immune cells. Yet characterization of immune cells within the naive brain compartment remains limited. In this study, we used CyTOF mass cytometry to characterize the immune populations of the naive mouse brain using 44 cell surface markers. By comparing immune cell composition and cell profiles between the brain compartment and blood, we were able to characterize previously undescribed cell subsets of CD8 T cells, B cells, NK cells and dendritic cells in the naive brain. Using flow cytometry, we show differential distributions of immune populations between meninges, choroid plexus and parenchyma. We demonstrate the phenotypic ranges of resident myeloid cells and identify CD44 as a marker for infiltrating immune populations. This study provides an approach for a system-wide view of immune populations in the brain and is expected to serve as a resource for understanding brain immunity.
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Article first published online: 20 DEC 2013 DOI: 10.1002/cyto.a.22429 © 2013 International Society for Advancement of Cytometry Via Alfredo Corell
Alfredo Corell's curator insight,
January 29, 2014 1:28 PM
This issue of Cytometry contains three reviews that further celebrate the longstanding, intense, and happy marriage between Cytometry and Immunology, as highlighted recently during the XV International Congress of Immunology, held in Milan, Italy, August 22–27, 2013. Scanning the scientific contributions of the Conference reveals that cytometric technology was used in the vast majority of abstracts and presentations. In other words, very few scientists did not switch on one or more lasers! It is obvious, that nowadays very few studies in the field of Immunology are possible without techniques based upon single cell analysis. |
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The International Clinical Cytometry Society and the European Society for Clinical Cell Analysis is proud to announce the first collaborative practical course focusing on clinical cytometry. Via Alfredo Corell
Alfredo Corell's curator insight,
July 23, 2014 8:05 AM
We welcome you to take part in this unique course offering that will focus on practical, hands-on, clinical flow cytometry education in a small group format. Content will focus on both basic fundamentals, as well as, specific clinical application. Most importantly, the final curriculum will be determined using feedback from the current course registrants to ensure an optimal experience for all attendees. |
