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T-reg or T-regulatory cells have direct roles in both autoimmunity and responses to pathogens. Here we'll show you how to quantify Tregs.
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Alfredo Corell
January 21, 2016 2:26 PM
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The standard Treg gating strategy for both mouse and human samples (after first gating out doublets and gating on live cells) includes the antigens CD3, CD4, CD25, FOXP3, and CD127.
When looking solely at antigen expression, Tregs are often defined as CD3+, CD4+, CD25hi, FOXP3+, and CD127lo (shown in the figure below as Option 1). Using these markers, a clear population is often visible from samples such as mouse splenocytes and human PBMC.