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The Effect of Recombinant Protein Production in Lactococcus lactis Transcriptome and Proteome

The Effect of Recombinant Protein Production in Lactococcus lactis Transcriptome and Proteome | iBB | Scoop.it

Lactococcus lactis is a food-grade, and generally recognized as safe, bacterium, which making it ideal for producing plasmid DNA (pDNA) or recombinant proteins for industrial or pharmaceutical applications. A paper published in Microorganisms by Sofia Duarte and Gabriel Monteiro from BERG-iBB reviews the major findings from L. lactis transcriptome and proteome studies, with an overexpression of native or recombinant proteins. These studies provide important insights on how to engineer the plasmid vectors and/or the strains in order to achieve high pDNA or recombinant proteins yields, with high quality standards. 

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Cover of Biotechnology Journal Highlights iBB's Work on Lactic Acid Bacteria

Cover of Biotechnology Journal Highlights iBB's Work on Lactic Acid Bacteria | iBB | Scoop.it

BERG-iBB researchers led by Gabriel Monteiro are engineering lactic acid bacteria and plasmid vectors in order to develop a flexible platform for biomolecule production. The cover image of the August issue of Biotechnology Journal highlights a recent contribution of the group. The work is part of the FCT-funded project LactoSynt.

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Sofia Duarte Joins LactoSynt Team as Research Scientist

Sofia Duarte Joins LactoSynt Team as Research Scientist | iBB | Scoop.it

Sofia Duarte has joined the team of the FCT-funded project "LactoSynt: Lactic Acid Bacteria as Cell Factories: a Synthetic Biology Approach for Plasmid DNA and Recombinant Protein Production". During the next three years Sofia will be responsible for engineering lactic acid bacteria and plasmid vectors with the goal of developing a flexible platform for biomolecule production. The project is a joint collaboration between iBB groups BERG (Gabriel Monteiro) and BSRG (Leonilde Moreira).

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Sofia Duarte Defends PhD Thesis in Biotechnology and Biosciences

Sofia Duarte Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Sofia de Oliveira Duarte has defended her Ph D thesis in Biotechnology and Biosciences at Instituto Superior Técnico on the 16th April 2018. During the last years, and under the supervision of Gabriel Monteiro from BERG-IBB and Lígia Rodrigues from University of Minho, Sofia has investigated the possibility of engineering lactic acid bacteria to produce plasmids and proteins. The title of her thesis is "Lactic acid bacteria as cell factories: A synthetic biology approach for plasmid DNA and recombinant protein production”.

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Plasmid Replicons for the Production of Biopharmaceuticals by Lactococcus lactis Cell Factories

Plasmid Replicons for the Production of Biopharmaceuticals by Lactococcus lactis Cell Factories | iBB | Scoop.it

While Lactococcus lactis is traditionally associated with the fermented food industry, applications of these bacteria have been spreading to the pharmaceutical industry. The goal is to use L. lactis as cell factories for the production of added-value recombinant proteins and plasmid DNA (pDNA) for DNA vaccination, as a safer and industrially profitable alternative to the traditional Escherichia coli host. In a recent review in International Molecular Sciences, Sofia Duarte and Gabriel Monteiro from BERG-iBB critically systematize the plasmid replicons available for the production of pharmaceutical-grade pDNA and recombinant proteins by L. lactis. The work is funded by the LactoSynt project.

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Increasing Plasmid Copy Number in Lactococcus lactis

Increasing Plasmid Copy Number in Lactococcus lactis | iBB | Scoop.it

The lipopolysaccharide-free Lactococcus lactis is an interesting alternative to E. coli for plasmid production. A key requirement for L. lactis‐based plasmid manufacturing is the availability of high‐copy number plasmids. In a recent publication in Biotechnology Journal, BERG-iBB researchers led by Gabriel Monteiro describe how engineering of the repDE Ribosome Binding Site led to an increase in the copy number  of plasmid pTRKH3 in L. lactis LMG19460 cells.  The number of copies of mutant pTRKH3‐b increased up to 215 copies per chromosome, which corresponds to a 3.5 fold increase when compared to the non‐modified pTRKH3. The new mutant is an important step towards the establishment of lactic acid bacteria as viable plasmid producers. The work was developed in the context of the FCT-funded, LactoSynt project.

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Effects of Clay's Chemical Interactions on Biocementation

Effects of Clay's Chemical Interactions on Biocementation | iBB | Scoop.it

Biocementation, or microbially induced calcite precipitation (MICP), is a technique currently being appraised for the improvement of sandy soils. A recent study led by Gabriel Monteiro from BERG-iBB and Rafaela Cardoso from IST describes an investigation of the effect of clay chemical interactions on biocementation with Sporosarcina pasteurii. Results show that osmotic effects play a key role in biocimentation and that MICP treatment in clayey soils is more complex than in sands with reduced clay content. Click on title to learn more about the research published in Applied Clay Science.

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