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Institute for Bioengineering and Biosciences
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Plasmid Replicons for the Production of Biopharmaceuticals by Lactococcus lactis Cell Factories

Plasmid Replicons for the Production of Biopharmaceuticals by Lactococcus lactis Cell Factories | iBB | Scoop.it

While Lactococcus lactis is traditionally associated with the fermented food industry, applications of these bacteria have been spreading to the pharmaceutical industry. The goal is to use L. lactis as cell factories for the production of added-value recombinant proteins and plasmid DNA (pDNA) for DNA vaccination, as a safer and industrially profitable alternative to the traditional Escherichia coli host. In a recent review in International Molecular Sciences, Sofia Duarte and Gabriel Monteiro from BERG-iBB critically systematize the plasmid replicons available for the production of pharmaceutical-grade pDNA and recombinant proteins by L. lactis. The work is funded by the LactoSynt project.

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Bruno Silva Joins LactoSynt Team as Research Fellow

Bruno Silva Joins LactoSynt Team as Research Fellow | iBB | Scoop.it

Bruno Silva has joined the team of the FCT-funded project "Lactosynt: Lactic acid bacteria as cell factories". During the next years Bruno will be responsible for the development of a lactic acid bacteria platform for the production of plasmids and recombinant proteins with therapeutic applications. Bruno is a microbial geneticist with a doctoral degree in Genetics from Universidade Federal de Minas Gerais. He has more than 8 years of research experience in microbiology and molecular biology with a focus on probiotic and lactic acid bacteria.

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Increasing Plasmid Copy Number in Lactococcus lactis

Increasing Plasmid Copy Number in Lactococcus lactis | iBB | Scoop.it

The lipopolysaccharide-free Lactococcus lactis is an interesting alternative to E. coli for plasmid production. A key requirement for L. lactis‐based plasmid manufacturing is the availability of high‐copy number plasmids. In a recent publication in Biotechnology Journal, BERG-iBB researchers led by Gabriel Monteiro describe how engineering of the repDE Ribosome Binding Site led to an increase in the copy number  of plasmid pTRKH3 in L. lactis LMG19460 cells.  The number of copies of mutant pTRKH3‐b increased up to 215 copies per chromosome, which corresponds to a 3.5 fold increase when compared to the non‐modified pTRKH3. The new mutant is an important step towards the establishment of lactic acid bacteria as viable plasmid producers. The work was developed in the context of the FCT-funded, LactoSynt project.

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Lactic Acid Bacterial Factories

Lactic Acid Bacterial Factories | iBB | Scoop.it

The project "LactoSynt: Lactic Acid Bacteria as Cell Factories: a Synthetic Biology Approach for Plasmid DNA and Recombinant Protein Production" has been recommended for funding by FCT (2017 Call for SR&TD Project Grants). The goal of LactoSynt is to engineer lactic acid bacteria and plasmid vectors in order to develop a flexible platform for biomolecule production. Applications in the pharmaceutical field (DNA vaccines, recombinant proteins) are envisaged. The project, which falls within the scientific area of Medical Biotechnology, is headed by Gabriel Monteiro from BERG-iBB and Leonilde Moreira from BSRG-iBB.

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José Santos Joins LactoSynt Team as Research Fellow

José Santos Joins LactoSynt Team as Research Fellow | iBB | Scoop.it

José Santos has joined the team of the FCT-funded "Lactosynt: Lactic acid bacteria as cell factories: a Synthetic Biology Approach for Plasmid DNA and Recombinant Protein Production”. Over the next year, José will be involved in the editing of the genome of lactic acid bacteria strains with the aim of developing an effective bacterial platform for production of biopharmaceutical-grade plasmid and recombinant proteins.

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Cover of Biotechnology Journal Highlights iBB's Work on Lactic Acid Bacteria

Cover of Biotechnology Journal Highlights iBB's Work on Lactic Acid Bacteria | iBB | Scoop.it

BERG-iBB researchers led by Gabriel Monteiro are engineering lactic acid bacteria and plasmid vectors in order to develop a flexible platform for biomolecule production. The cover image of the August issue of Biotechnology Journal highlights a recent contribution of the group. The work is part of the FCT-funded project LactoSynt.

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Sofia Duarte Joins LactoSynt Team as Research Scientist

Sofia Duarte Joins LactoSynt Team as Research Scientist | iBB | Scoop.it

Sofia Duarte has joined the team of the FCT-funded project "LactoSynt: Lactic Acid Bacteria as Cell Factories: a Synthetic Biology Approach for Plasmid DNA and Recombinant Protein Production". During the next three years Sofia will be responsible for engineering lactic acid bacteria and plasmid vectors with the goal of developing a flexible platform for biomolecule production. The project is a joint collaboration between iBB groups BERG (Gabriel Monteiro) and BSRG (Leonilde Moreira).

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