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Adaptation to Acetic Acid Stress Involves Structural Alterations and Increased Stiffness of the Yeast Cell Wall

Adaptation to Acetic Acid Stress Involves Structural Alterations and Increased Stiffness of the Yeast Cell Wall | iBB | Scoop.it

The role of the cell wall in yeast response and tolerance to stress is frequently neglected. A BSRG-iBB research paper just published in Scientific Reports, provides, for the first time, a comprehensive view of the alterations occurring at the cell wall in a yeast population adapting to sub-lethal stress induced by acetic acid. The results reveal changes to the cell wall polysaccharide composition and nanomechanical properties, as well as alterations in the transcript levels of key cell wall biosynthetic genes. This paper reinforces the notion that the adaptive yeast response to acetic acid involves coordinated alterations of the cell wall at the biophysical and molecular levels. The gathered knowledge is important for the design of superior industrial strains and for the efficient control of the deleterious activity of spoilage yeasts, particularly in the Food Industry. This research work is first-authored by the PhD student of the PhD programme in Biotechnology and Biosciences Ricardo Ribeiro (FCT_DP AEM fellowship), performed under the supervision of Isabel Sá-Correia. This collaborative study with Fábio Fernandes (BSIRG-iBB) and Mário S. Rodrigues and his team (BioISI, Faculty of Sciences, ULisboa), is also coauthored by Cláudia Godinho (posdoc researcher) and the PhD student Nuno Bourbon-Melo (FCT_DP BIOTECnico) from the BSRG-iBB team.

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Luís Martins to Defend PhD Thesis in Biotechnology and Biosciences

Luís Martins to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Luís Miguel Candeias Martins will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, Tuesday the 18th of May 2021, at 14:00 (https://videoconf-colibri.zoom.us/j/82200706553). During the last years, and under the supervision of Prof. Isabel Sá-Correia from BSRG-iBB, Luís studied the genetic tolerance determinants for multiple stresses in S. cerevisiae and the potential of oleaginous red yeasts for the efficient carbon substrates conversion present in pectin-rich hydrolysates. The title of his thesis is “Valorisation of pectin-rich agro-industrial residues by yeasts: Potential and Challenges”.

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BIOTECnico PhD Alumni Seminar: Pedro Pais

BIOTECnico PhD Alumni Seminar: Pedro Pais | iBB | Scoop.it

Pedro Pais will be giving a talk entitled "Unveiling the complex regulatory network governing antifungal drug resistance in Candida glabrata: from new regulators to new effectors", Tuesday the 16th february at 16:00 h via zoom. Pedro obtained his PhD in Biotechnology and Biosciences in 2019 and is currently an Assistant Researcher at iBB.

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BIOTECnico PhD Program Seminar Cycle 2021

BIOTECnico PhD Program Seminar Cycle 2021 | iBB | Scoop.it

The BIOTECnico PhD program will initiate a cycle of alumni seminars on the 19th of January 2021. Until the 30th of March, every 2 weeks, on Tuesdays at 4 pm, BIOTECnico alumni will deliver a 20 min presentation describing the main achievements of the PhD projects they developed at iBB, as well as their current professional activities and institution. The seminars will be delivered via zoom and the full list can be found here.

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Use of Non-conventional Yeasts to Enrich the Aroma Profile of Beer

Use of Non-conventional Yeasts to Enrich the Aroma Profile of Beer | iBB | Scoop.it

There is a wide diversity of non-conventional yeast species, which often present distinctive metabolic properties and can therefore impart different flavours and aromas to beer. Because of this, brewers now regard non-conventional yeast species as a tool to differentiate their beers from other products in the market. In fact, some of these yeasts are already used to produce specific beer styles, such as lambics. A study recently published in Food Microbiology, coordinated by Dr. Margarida Palma and Prof. Isabel Sá-Correia and first authored by the BIOTECnico PhD student Nuno Melo, investigates how two non-conventional yeast species commonly found in wineries, Hanseniaspora guilliermondii and Hanseniaspora opuntiae, influence the volatile composition of beer. These two species, initially selected by a preliminary organoleptic evaluation, were found to modulate the aroma profile of beer, namely by increasing phenylethyl acetate concentration (‘rose’, ‘honey’ aroma). These findings highlight the importance of non-conventional yeasts in shaping the aroma profile of beer and suggest a role for Hanseniaspora spp. in improving it.

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Monitoring Proteolytic Activity in Real-Time

Monitoring Proteolytic Activity in Real-Time | iBB | Scoop.it

Proteases play a pivotal role in biological processes, from digestion, cell proliferation and differentiation to fertility. Deregulation of protease metabolism can result in several pathological conditions (i.e., cancer, neurodegenerative disorders, etc). Monitoring proteolytic activity in real-time can ultimately help us understand the role of such enzymes within a certain condition. This may contribute to an early diagnosis, the follow-up of disease progression or the development of protease-targetted treatments (e.g. the current HIV treatment). In a recent paper published in Trends in Biochemical Sciences, Rui Oliveira-Silva and colleagues from BERG, CQE and University of Aveiro review the main approaches used to develop biosensors for monitoring proteolytic activity. A comparison of the advantages and disadvantages of each approach is provided along with a discussion of their importance and promising opportunities for the integration of such sensors into Personalized Medicine.

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Assembly of Dyes at the Tips of Gold Nanorods Enhances Fluorescence Emission

Assembly of Dyes at the Tips of Gold Nanorods Enhances Fluorescence Emission | iBB | Scoop.it

The conjugation of dye-labelled DNA oligonucleotides with gold nanorods has been widely explored for the development of multifunctional fluorescent nanoprobes. In a recent publication in Nanoscale, BERG-iBB researchers in collaboration with colleagues from Centro de Química Estrutural and Centro de Ciências do Mar show that the functionalization route is crucial to achieve enhanced emission in dye nano-assemblies based on gold nanorods. By using a tip-selective approach for thiol attachment of dye molecules onto gold nanorods, it was possible to effectively increase the emission by more than 10-fold relatively to that of free dye. The results could improve significantly the performance of dye nano-assemblies for imaging or sensing applications.The work is part of the PhD thesis of David Botequim, which is supervised by Pedro Paulo (CQE) and Miguel Prazeres (BERG-iBB).

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Nuno Bourbon Delivers Oral Presentation at Microbiotec’19

Nuno Bourbon Delivers Oral Presentation at Microbiotec’19 | iBB | Scoop.it

Nuno Bourbon, PhD student from the BIOTECnico doctoral programme at iBB-BSRG, presented his work during the symposium on “Industrial and Food Microbiology” of the Congress of Microbiology and Biotechnology 2019 (Microbiotec’19), which was held between the 5th and 7th of December 2019 in Coimbra, Portugal. The presentation, entitled “Use of Hanseniaspora guilliermondii and Hanseniaspora opuntiae in co-fermentation with Saccharomyces cerevisiae to enhance the aromatic profile of craft beer”, focused on the potential of using two distinct Hanseniaspora yeast species to improve the organoleptic properties of beer. This work was carried out under the supervision of Prof. Isabel Sá-Correia (iBB-BSRG) and Dr. Margarida Palma (iBB-BSRG), in collaboration with Prof. Maria Rosário Bronze’s team (IBET and Faculty of Pharmacy, ULisboa, for the analysis of volatile compounds).

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Featured Photo: Illustration of DNA Origami Nanotetrahedron

Featured Photo: Illustration of DNA Origami Nanotetrahedron | iBB | Scoop.it

Description: Illustration of a DNA origami nanotetrahedron created with David Goodsell’s Illustrate software.

 

Context: BIOTecnico PhD student Ana Rita Santos, under the supervision of BERG-iBB researcher Miguel Prazeres is developing processes to produce and purify DNA scaffolds and DNA origami nano-objects.

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Pedro Pais to Defend PhD Thesis in Biotechnology and Biosciences

Pedro Pais to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Pedro Henrique Magalhães Fernandes Pais will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, friday the 27th september 2019 (10:00 H, room PA-3). During the last years, and under the supervision of Miguel Teixeira from BSRG-iBB, Pedro studied the intricacies of the regulatory network that controls drug resistance in the fungus Candida glabrata. The title of his thesis is "Unveiling the complex regulatory network governing antifungal drug resistance in Candida glabrata: from new regulators to new effectors".

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André Nascimento Delivers Oral Presentation at Affinity 2019

André Nascimento Delivers Oral Presentation at Affinity 2019 | iBB | Scoop.it

André Nascimento from BERG-IBB delivered an oral presentation at the Affinity 2019 Conference titled “Debottlenecking process development using a microfluidic high-throughput platfrom”. The work involves a collaboration between researchers from BERG-iBB and the Rensselaer Polytechnic Institute. The meeting brought together scientists and engineers from academia and industry to exchange knowledge in the field of affinity technology, molecular recognition and its application in industry for health and disease, bioprocessing and biosensing. The conference took place in Stockholm on the 26-18 th June.

Aptech Visa's comment, July 9, 2019 8:36 AM
Saskatchewan Express Entry
https://www.aptechvisa.com/sinp-saskatchewan-express-entry
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Mónica Galocha Receives Best Poster Award at HFP2019

Mónica Galocha Receives Best Poster Award at HFP2019 | iBB | Scoop.it

Mónica Galocha, PhD student from the PhD program AEM-Applied Environmental Microbiology, won one of the PhD poster prizes awarded at the eight FEBS Advance Lecture Course on Human Fungal Pathogens-Molecular Mechanisms of Host-Pathogen Interactions and Virulence, held on May 18-24, in La Colle sur Loup, France, with a communication entitled “Candida glabrata evolution towards posaconazole resistance irrespective of PDR1 GOF mutations”. The work results from a collaboration between researchers from BSRG-iBB (Miguel Cacho Teixeira), University of Porto (Acácio G. Rodrigues) and Chiba University (Hiroji Chibana).

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Applications for the FCT-PhD Program on Biotechnology and Biosciences Open

Applications for the FCT-PhD Program on Biotechnology and Biosciences Open | iBB | Scoop.it

The 2019 call for applications to the FCT-PhD Program on Biotechnology and Biosciences (BIOTecnico) is open from the 29th march until the 12th may 2019. BIOTEcnico is an interdisciplinary four-year’s Doctoral program, offered by IST and iBB, that provides PhD students with a world-class training in Biotechnology and Biosciences. The course is designed to attract and prepare talented and motivated students to translate basic bioscience discoveries into technological innovations in Industrial, Agro-Food, Health and Environmental Biotechnology. Learn more at BIOTecnico's website.

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Determination of Recombination Efficiency in Minicircle Production by Real-time PCR

Determination of Recombination Efficiency in Minicircle Production by Real-time PCR | iBB | Scoop.it

The determination of the recombination efficiency and the monitoring of parental plasmids (PP), minicircles (MC) and miniplasmid (MP) species during processing and in the final product are critical aspects of minicircle manufacturing. A paper published in Analytical Biochemistry by BERG-iBB researchers Cláudia Alves, Miguel Prazeres and Gabriel Monteiro describes a real-time PCR method for the specific identification of PP, MP or MC that uses sets of primers specific for each species. The results presented indicate that the method can be applied for the determination of recombination efficiency in pure DNA samples.

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BIOTECnico PhD Alumni Seminar: Mafalda Cavalheiro

BIOTECnico PhD Alumni Seminar: Mafalda Cavalheiro | iBB | Scoop.it

Mafalda Cavalheiro will be giving a talk entitled "Deciphering Mechanisms of Pathogenesis in Candida glabrata: in the Crossroad Between Drug Resistance and Virulence ", Tuesday the 16th of March at 16:00 h via zoom. Mafalda obtained her PhD in Biotechnology and Biosciences in 2020 and is currently working at A4F – Algae for Future, as a Junior R&D Project Manager.

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BIOTECnico PhD Alumni Seminar: Amir Hassan

BIOTECnico PhD Alumni Seminar: Amir Hassan | iBB | Scoop.it

Amir Hassan will be giving a talk entitled "Adaptation of Burkholderia cepacia complex bacteria to the cystic fibrosis lung: focus on lipopolysaccharide O-antigen and cell wall physical properties", Tuesday the 19th january at 16:00 h via zoom. Amir obtained his PhD in Biotechnology and Biosciences in 2020 and is currently at the Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.

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Andreia Pimenta to Defend PhD Thesis in Biotechnology and Biosciences

Andreia Pimenta to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Andreia Isabel de Melo Pimenta will be defending her PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, Tuesday the 22nd of December 2020, at 10:00 am (https://videoconf-colibri.zoom.us/j/87589426644). During the last years, and under the supervision of Arsénio Fialho from BSRG-iBB, Andreia studied the role of Trimeric autotransporter adhesins in the pathogenesis of Burkholderia cenocepacia. The title of her thesis is “New insights into the interaction of Burkholderia cenocepacia with host cells: Trimeric autotransporter adhesins as pathogenicity factors”.

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David Botequim to Defend PhD Thesis in Biotechnology and Biosciences

David Botequim to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

David José Roxo Botequim will be defending his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, Wednesday the 18th of November 2020, at 10:30 am (https://videoconf-colibri.zoom.us/j/89168693131). During the last years, and under the supervision of Pedro Paulo from CQE and Miguel Prazeres from BERG-iBB, David studied fluorescently-labeled DNA probes conjugated onto gold nanoparticles for the improved detection of nucleic acids. The title of his thesis is “Fluorescently-labeled DNA Probes Functionalized onto Gold Nanorods for Signal Enhancement in Nucleic Acids’ Detection”.

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André Nascimento Defends PhD Thesis in Biotechnology and Biosciences

André Nascimento Defends PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

André Nascimento defended his PhD thesis in Biotechnology and Biosciences on Monday the 6th april 2020 (14:00 H). During the last years, and under the supervision of Ana Azevedo from BERG-iBB and Steven Cramer and Pankaj Karande from the Rensselaer Polytechnic Institute (US), André developed and explored affinity peptides and multimodal ligands for the purification of Fab antibody fragments. The title of the thesis is “Development of a novel purification platform for Fab antibody fragments based on affinity peptides and multimodal ligands”.

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Amir Hassan to Defend PhD Thesis in Biotechnology and Biosciences

Amir Hassan to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Ahmed Amir Elsayed Hassan will be defending her PhD thesis in Biotechnology and Biosciences on tuesday the 28th january 2020 (14:30 H, amphitheater PA-3, Mathematics Building). During the last years, and under the supervision of Isabel Sá-Correia from BSRG-iBB, Amir studied the role of the lipopolysaccharide O-antigen on the adaptation of the Bhurkolderia cepacia complex to the cystic fibrosis environment. The title of the thesis is “Adaptation of Bhurkolderia cepacia complex bacteria to the cystic fibrosis lung: focus on lipopolysaccharide O-antigen and cel wall physical properties”.

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Variation of Burkholderia cenocepacia Cell Wall Morphology and Mechanical Properties During Cystic Fibrosis Chronic Lung Infection, Assessed by AFM

Variation of Burkholderia cenocepacia Cell Wall Morphology and Mechanical Properties During Cystic Fibrosis Chronic Lung Infection, Assessed by AFM | iBB | Scoop.it

Bacterial cell envelope plays a central role in cell physiology and the alteration of surface properties can implicate the variation of phenotypes that play a crucial role in the pathogenesis of infectious diseases, such as the resistance to antibiotics and other environmental stresses, biofilm formation, persistence of infection. However, the influence that Burkholderia cenocepacia adaptive evolution during long-term respiratory infection in cystic fibrosis (CF) patients has on cell wall morphology and mechanical properties is poorly understood. This study, just published in the journal Scientific Reports, has examined cell wall morphology and mechanical properties of three sequential B. cenocepacia clonal variants by Atomic Force Microscopy (AFM). Results reinforce the concept of the occurrence of phenotypic variation and adaptive evolution during chronic infection, also at the level of cell size, form, envelope topography and physical properties. This research work, coordinated by Prof. Isabel Sá-Correia from iBB-BSRG and IST, was the result of a national collaboration with the team of Dr. Mário S. Rodrigues from BioISI, Faculty of Sciences, Universidade de Lisboa, having the IST PhD student of the BIOTECnico program Amir Hassan, from iBB-BSRG, as first author of the publication.

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Oscillatory Reactor for Continuous ATPS Presented at ICB IV

Oscillatory Reactor for Continuous ATPS Presented at ICB IV | iBB | Scoop.it

Diogo Faria, PhD candidate at BIOTECnico Programme in IBB-BERG, gave a poster presentation at the conference "Integrated Continuous Biomanufacturing IV", organized by ECI (Engineering Conferences International), held in Cape Cod, Massachusetts, in October 6-9. The poster was about Continuous Aqueous Two-Phase System Extraction using Oscillatory Flow Reactor. The work was carried out under the supervision of Prof. Ana Azevedo (IBB-BERG) and Prof. Raquel Aires Barros (iBB-BERG), with colaboration of António Ferreira (FEUP).

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Ricardo Ribeiro Delivers Oral Presentation at the 7th Conference on Physiology of Yeast and Filamentous Fungi

Ricardo Ribeiro Delivers Oral Presentation at the 7th Conference on Physiology of Yeast and Filamentous Fungi | iBB | Scoop.it

Ricardo Ribeiro, PhD student from the PhD program AEM - Applied

Environmental Microbiology delivered an oral presentation at the 7th Conference on Physiology of Yeast and Filamentous Fungi titled “Yeast response and tolerance to acetic acid: focus on the cell envelope” The work involves a collaboration between researchers from BSRG-iBB and BioISI. The meeting brought together researchers to exchange knowledge in the field of applied

physiology of yeasts and filamentous fungi, boosting the  understanding of these systems, envisaging their application in biotechnological, food, beverage and pharmaceutical industries. The conference took place in Milan, Italy, on the 24th-27th June.

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Density Gradient Selection of Silver Nanotriangles

Density Gradient Selection of Silver Nanotriangles | iBB | Scoop.it

Many metal nanoparticles exhibit plasmonic properties that can be explored in different applications. The synthesis of these nanoparticles often leads to a heterogeneous mixture in terms of sizes and shapes that needs to be fractionated to yield samples with narrow plasmon resonances. A simple method based on sucrose density gradient centrifugation has been proposed by BERG-iBB researchers and colleagues from Centro de Química Estrutural and Universidade de Aveiro for the fractionation of colloidal silver nanotriangles. The method affords particle fractions with surface plasmon resonances spanning from red to infrared spectral ranges that could be used to tune optical properties for plasmonic applications. The work is part of the PhD thesis of Rui Oliveira Silva and was published in Nanomaterials.

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Featured Photo: Bacteriophage Plaque Assay

Featured Photo: Bacteriophage Plaque Assay | iBB | Scoop.it

Description: A plaque used to determine bacteriophage titers, featured photo by Jorge João, Copyright BERG-iBB 2018.

 

Context: BERG researchers led by Ana Azevedo and Miguel Prazeres are developing processes for bacteriophage manufacturing in collaboration with the portuguese start-up Tecnophage. The work is supported by the Portugal2020 project "Phage4BacID-Bacteriophages in the Diagnostic and Prevention of Multiresistant Infection in Portugal and in the World".

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