Vectorology - GEG Tech top picks

In this study, the authors design a lentiviral vectors (LV) for delivery of snoMEN RNAs which is an antisense technology for targeted knock-down of protein coding mRNAs. They show that the vectorization in LV increases the efficiency of delivery in many human cell lines that are difficult to transfect with plasmid vectors. Transduction of lentiviral vectors expressing snoMEN targeted to pri-miR21 induces apoptosis in human lung adenocarcinoma cells, which express high levels of miR21, but not in human primary cells. They show that snoMEN-mediated suppression of miRNA expression is prevented by siRNA knock-down of Ago2, but not by knock-down of Ago1 or Upf1. snoMEN RNAs colocalise with Ago2 in cell nuclei and nucleoli and can be co-immunoprecipitated from nuclear extracts by antibodies specific for Ago2.

www.geg-tech.com/Vectors

Depression is a serious and potentially life-threatening mental disorder with unknown etiology. Here, the authors used lentiviral vectors to over expressed BDNF, miR-182 or miR-182 silencer in the hippocampus of rat. They observe that LV-BDNF or LV-si-miR-182 have anti-depressant like effect. In contrast, miR-182 overexpression exacerbated depression-like behaviors and decreased BDNF.  Taken together, the current results reveal a potential molecular regulation of miR-182 on BDNF and the pronounced behavioral consequences of this regulation.

www.geg-tech.com/Vectors