Scientists compared selection and expression efficacies in cells cotransduced with separate single-gene MGMT and GFP lentivectors to those obtained with dual-gene vectors employing either internal ribosome entry site (IRES) or 2A elements for co-expression strategies. The highest dual-gene expression (MGMT+GFP+) percentages were obtained with the 2A dual-gene vector, but half of the resulting gene products existed as fusion proteins. Equivalent MGMT expression levels were obtained with each strategy, but GFP expression levels derived from the IRES dual-gene vector were significantly lower.
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Scientists compared selection and expression efficacies in cells cotransduced with separate single-gene MGMT and GFP lentivectors to those obtained with dual-gene vectors employing either internal ribosome entry site (IRES) or 2A elements for co-expression strategies. The highest dual-gene expression (MGMT+GFP+) percentages were obtained with the 2A dual-gene vector, but half of the resulting gene products existed as fusion proteins. Equivalent MGMT expression levels were obtained with each strategy, but GFP expression levels derived from the IRES dual-gene vector were significantly lower.
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