Adjuvant poly(N-isopropylacrylamide) generates more efficient monoclonal antibodies against truncated recombinant histidine-rich protein 2 of Plasmodium falciparum for malaria diagnosis - Verma - B... | Immunology and Biotherapies | Scoop.it
From onlinelibrary.wiley.com - January 10, 2015 2:36 PM
Adjuvant poly(N-isopropylacrylamide) generates more efficient monoclonal antibodies against ... http://t.co/HAtCVrK52x #biotech #science

 

Abstract

Adjuvants play an important role in eliciting immune responses and subsequent generation of antibodies with high specificity. Recently, poly(N-isopropylacrylamide) (PNiPAAm), also known as a “smart” polymer, has been proposed as a potential adjuvant for making antibodies and vaccines. This material exhibits efficient delivery, protection against degradation, and preservation of antigen epitopes. In this work, we used both CFA and smart polymer to develop a highly specific murine monoclonal antibody (mAb) against recombinant truncated histidine rich protein2 (HRP2) of P.falciparum. Our results indicate that the mAbs developed using these adjuvants were able to recognize recombinant HRP2 and native PfHRP2 protein from spent medium. The mAbs generated against recombinant truncated HRP2 showed better sensitivity to the antigen and importantly mAbs generated through PNiPAAm adjuvant were in the range of 108 to 109 M-1. The mAbs generated through PNiPAAm are very efficient and sensitive in detecting HRP2. To the best of our knowledge, this is the first report of such comparison having been made between these two adjuvants and we propose that the smart polymer has huge potential as an alternative to CFA. Additionally, we discuss the utility of the mAbs generated through PNiPAAm for specific diagnosis of malaria caused by P.falciparum.

 


Via Krishan Maggon