iBB

The ability to analyze the distribution of topoisomers in a plasmid DNA sample is important when evaluating the quality of preparations intended for gene therapy and DNA vaccination, or when performing biochemical studies on the action of topoisomerases and gyrases. In a recent paper in Analytical Biochemistry, BERG-iBB researchers led by Miguel Prazeres and Ana Azevedo we describe the separation of supercoiled (sc) and open circular (oc) topoisomers by multimodal chromatography. A medium modified with the ligand N-benzyl-N-methyl ethanolamine and an elution scheme with increasing NaCl concentration are used to accomplish the baseline separation of sc and oc plasmid. Click on title to learn more.