from Flow Cytometry to Cytomics

There are several commercial kits available for the determination of residual leukocytes (rWBC) in different blood derived products. All of then based on the fact that nucleated cells could be stained by Propidium Iodide. Identification of stained cells could be assayed by flow cytometry or other cassette technologies:

 

MEASUREMENT OPTIONS:

BD-LEUCOCOUNT: http://www.bdbiosciences.com/ptProduct.jsp?prodId=21185&catyId=21157&page=product

 

BECKMAN-COULTER LEUKOSURE ENUMERATION KIT: http://ow.ly/fueUl

 

CYTOGNOS LEUCOFINDER: http://www.cytognos.com/index.php/en/reagents-flow-cytometry/absolute-counts/1238-leucofinder-for-rwbc

 

HAND-MADE METHOD WITH INVITROGENE VybrantDyeCycleViolet: http://www.google.es/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&ved=0CD4QFjAB&url=http%3A%2F%2Fwww.invitrogen.com%2Fetc%2Fmedialib%2Ffiles%2FCell-Analysis%2FPDFs.Par.31561.File.dat%2FCYTO2012ANovelNonWash.pdf&ei=Ak-tUNS_OOG50QX184HYAw&usg=AFQjCNHbOvtPfZ3l_glJAQDoKQ_DM1si3g

 

The manual traditional method was the Nageotte Chamber: http://www.google.es/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&ved=0CC4QFjAA&url=http%3A%2F%2Fwww.hausserscientific.com%2Fproducts%2Fnageotte_chamber.html&ei=21CtUNbOKarK0QWypoCgCA&usg=AFQjCNHNeMnmVcxfXmhsEV6h8uQiQpslPw

 

QUALITY CONTROL:

The GECLID-SEI External Quality Control for Proficiency Testing in rWBC:

http://www.geclidsei.uva.es

 

EPTIS page: http://www.eptis.bam.de/php/eptis/index_results1.php?task=show__search_summary&ringversuch_id=3719&ringversuch_treffer=,GENERAL,NAME&level_id_of_hitlist=1&keyword__not_refined=residual%20leukocytes&keywordstartingposition__not_refined=1&countries__not_refined=__&productgroups__not_refined=-1&testingfields__not_refined=-1&accredited__not_refined=0&rv_ids__not_refined=&sorting_order__bez=&sorting_order__veranst=&sorting_order__matchcount=DESC&sorting_order__aktdatum=&resultpage_selected=1&resultpage_last=1

 

 

 

 

 

 

Via Alfredo Corell