Virology News
65.6K views | +0 today
Follow
 
Scooped by Ed Rybicki
onto Virology News
Scoop.it!

DNA Preparation Tubes Contaminated with Novel ssDNA Virus

DNA Preparation Tubes Contaminated with Novel ssDNA Virus | Virology News | Scoop.it
A novel virus thought to have come from human samples appears to have been derived from seawater during the manufacture of tubes used to extract DNA.  
Ed Rybicki's insight:

I have a problem with the original report, in J Virol (http://jvi.asm.org/content/early/2013/09/05/JVI.02323-13.abstract?related-urls=yes&legid=jvi;JVI.02323-13v1): not that they discovered it, because that was done well.  However, they essentially REdiscovered something that was ±100% identical to a virus already sequenced and named by Chinese researchers - who did not use the Qiagen kits, apparently - and then gave it a new name!

 

Sorry, that is simply bad practice!  It also smacks of scientific imperialism of a sort that characterised early discovery work on HTLVs and on HIV, when US researchers calmly treated earlier characterisations as if they had never happened.

 

There is another leap that I do not think is justified: the authors claim that

 

"Analysis of environmental metagenome libraries detected PHV sequences in coastal marine waters of North America, suggesting that a potential association between PHV and diatoms (algae) that generate the silica matrix used in the spin columns may have resulted in inadvertent viral contamination during manufacture".


Really?  On the basis of presence of a sequence in a metagenomic trawl?  No resampling with specific primers on a fresh sample?   And surely the generation of the silica matrix is done under conditions that would totally destroy adventitiious DNA?


So - an interesting paper, and a valuable notification (although it might have been nicer if they'd shared their findings informally, to save other people like our Virology Diagnostics lab time and money).  But flawed, in my opinion.

No comment yet.
Virology News
Topical news snippets about viruses that affect people.  And other things. Like Led Zeppelin. And zombies B-)
Curated by Ed Rybicki
Your new post is loading...
Your new post is loading...
Scooped by Ed Rybicki
Scoop.it!

The Vaccine Makers Project

The Vaccine Makers Project | Virology News | Scoop.it
Materials are updated as needed. The Vaccine Education Center staff regularly reviews materials for accuracy. You should not consider the information in this site to be specific, professional medical advice for your personal health or for your family's personal health.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Europe’s avian flu outbreak total in poultry passes 1,700

Europe’s avian flu outbreak total in poultry passes 1,700 | Virology News | Scoop.it
The amount of highly pathogenic avian influenza outbreaks in Europe in 2021 quadrupled that of 2020.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

A repository of stories of anti-vaxxers who died or came close to dying of COVID.

A repository of stories of anti-vaxxers who died or came close to dying of COVID. | Virology News | Scoop.it
A repository of stories of anti-vaxxers who died or came close to dying of COVID.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Drug treats Parkinson's, then makes it worse

Drug treats Parkinson's, then makes it worse | Virology News | Scoop.it
A new study clarifies how L-dopa, the front-line drug for Parkinson's disease, also contributes to the rapid decline in quality of life over time.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Horrified Anti-Vaxxer Discovers Every American Who Got Smallpox Vaccine In 19th Century Now Dead

Horrified Anti-Vaxxer Discovers Every American Who Got Smallpox Vaccine In 19th Century Now Dead | Virology News | Scoop.it
LYNCHBURG, VA—Astounded by the damning information, local anti-vaxxer Pete Dixon was reportedly horrified Thursday after discovering that every single American who got a smallpox vaccine in the 19th century was now deceased.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Itaconate and derivatives reduce interferon responses and inflammation in influenza A virus infection

Author summary Interferon responses are part of the primary host defenses against infections. However, excessive inflammation is often a major factor in severe disease or even death in respiratory infections such as influenza, as it can lead to acute respiratory distress syndrome and sepsis-like...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Return of the flu: EU faces threat of prolonged 'twindemic'

Return of the flu: EU faces threat of prolonged 'twindemic' | Virology News | Scoop.it
Influenza has returned to Europe at a faster-than-expected rate this winter after almost disappearing last year, raising concerns about a prolonged "twindemic" with COVID-19 amid some doubts about the effectiveness of flu vaccines.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Covid-flu joint booster vaccine possible late 2023: Moderna

Covid-flu joint booster vaccine possible late 2023: Moderna | Virology News | Scoop.it
Moderna aims to roll out a combined Covid-flu-RSV booster vaccine in late 2023, the US pharmaceutical firm said Monday, hoping a joint jab...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

H5N1 Flu Outbreak In Burkina Faso

H5N1 Flu Outbreak In Burkina Faso | Virology News | Scoop.it
Flu outbreaks continue to be a major crisis in the poultry industry.The year didn't start so well for poultry farmers in Burkina Faso, not only did it affect...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

With Covid, we’ll never have a normal flu season again

With Covid, we’ll never have a normal flu season again | Virology News | Scoop.it
This terrible Covid and flu season is a preview of the post-pandemic normal.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

CORBEVAX, a new patent-free COVID-19 vaccine, could be a pandemic game changer globally

CORBEVAX uses recombinant DNA technology that many countries already have the infrastructure to produce. Artis777 via iStock/Getty Images PlusThe world now has a new COVID-19 vaccine in its arsenal, and at a fraction of the cost per dose.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Development and Clinical Validation of a Potential Penside Colorimetric Loop-Mediated Isothermal Amplification Assay of Porcine Circovirus Type 3 

Development and Clinical Validation of a Potential Penside Colorimetric Loop-Mediated Isothermal Amplification Assay of Porcine Circovirus Type 3  | Virology News | Scoop.it
Porcine circovirus type 3 (PCV3), a novel circovirus, imposes great burdens on the global pig industry. The penside tests for detecting PCV3 are critical for assessing the epidemiological status and working out disease prevention and control programs due to the unavailability of a commercial...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

J&J booster 85% effective against Omicron hospitalisation, South Africa says

J&J booster 85% effective against Omicron hospitalisation, South Africa says | Virology News | Scoop.it
A Johnson and Johnson COVID-19 vaccine booster shot is 85% effective in protecting against being hospitalised by the Omicron variant for 1-2 months after it is received, the head of South Africa's Medical Research Council (SAMRC) said on Friday.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Persistence of T Cell and Antibody Responses to SARS-CoV-2 Up to 9 Months after Symptom Onset 

Persistence of T Cell and Antibody Responses to SARS-CoV-2 Up to 9 Months after Symptom Onset  | Virology News | Scoop.it
Flow cytometric data were analyzed using FlowJo version 10.7.1 (BD Biosciences). Multiplex cytokine bead data were analyzed using the LEGENDplex Data Analysis Software Suite (BioLegend). Statistical analyses were performed using GraphPad Prism version 9.1.1. Δ in figures represents values for which the corresponding background signal for each donor had been subtracted. “Background signal” is defined as the frequency of cells expressing the parameter being analyzed, or concentration of an analyte, in negative control wells. For each donor, the response is reported as the Δ after background subtraction for that donor. A T cell response was defined as positive if the value was two times background for that sample, and above a minimal level of 0.003%, which is calculated as the median of all DMSO controls. To calculate the rate of decay of the CD4 T cell responses based on ICC responses, we focused only on the stronger responses, and we only included donors whose response was 2 SDs above the median background. For proliferation data, in calculating the distribution of CD4 or CD8 subsets, only positive results were included, defined as above. For the multiplex data, the limit of detection was established using a standard curve for each analyte. Pairwise comparisons were made by a two-tailed Wilcoxon test or a nonparametric Dunn’s multiple comparisons test. Correlation analyses were performed by computing the Spearman correlation coefficient. For longitudinal data, the percentage change over time and t1/2 were calculated after linear regression was performed for each donor. We report the average percentage change per week and the average t1/2. Cluster analysis for multiplex data were performed by first calculating fold change in response to an Ag and then performing hierarchical agglomerative clustering on log-transformed data. Values across donors were standardized by minimum/maximum normalization such that, for each analyte, the minimum is subtracted, then divided by its maximum. These data were visualized using the Seaborn data visualization library for Python (https://joss.theoj.org/papers/10.21105/joss.03021).
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Moderna CEO Hints At Possible 3-in-1 COVID-19/Flu/RSV Vaccine by Fall

Moderna CEO Hints At Possible 3-in-1 COVID-19/Flu/RSV Vaccine by Fall | Virology News | Scoop.it
​Moderna is poised to commercialize its COVID-19 and influenza combination vaccine by the fall of 2023, with some potential for the same drug to also be viable against the RSV.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Evaluation of the relative virulence of novel SARS-CoV-2 variants: a retrospective cohort study in Ontario, Canada

The increasing virulence of SARS-CoV-2 VOCs will lead to a considerably larger, and more deadly, pandemic than would have occurred in the absence of the emergence of VOCs.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Yes, you can catch the flu and Covid. No, 'flurona' isn't real.

Yes, you can catch the flu and Covid. No, 'flurona' isn't real. | Virology News | Scoop.it
It would have been hard to know in March 2020 that Covid-19 testing limitations in New York City would help lead doctors at Montefiore Medical Center in the Bro...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

The unequal impact of the Spanish flu in South Africa

The unequal impact of the Spanish flu in South Africa | Virology News | Scoop.it
The 1918 Influenza epidemic in South Africa exacerbated existing large racial inequalities in access to institutionalized medical treatment...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

No vaccine, no French Open for Djokovic as rules tighten

No vaccine, no French Open for Djokovic as rules tighten | Virology News | Scoop.it
Novak Djokovic risks being frozen out of tennis as he chases a record 21st Grand Slam title, with rules on travellers who are unvaccinated against COVID-19 tightening in the third year of the pandemic and some tournaments reconsidering exemptions.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

France to push EU states to approve avian flu vaccine for poultry

France to push EU states to approve avian flu vaccine for poultry | Virology News | Scoop.it
Two vaccines will soon be tested in France in response to the worst avian influenza outbreak Europe has ever faced, French Agriculture Minister Julien Denormandie has announced. The aim is for them to be approved for all 27 EU member states.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Israeli trial, world's first, finds 4th dose ‘not good enough’ against Omicron

Expert at Sheba Medical Center says jab raises COVID antibody levels, but there are ‘still a lot of infections’ among those who received it...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Queen’s swans culled after bird flu outbreak on the Thames

Queen’s swans culled after bird flu outbreak on the Thames | Virology News | Scoop.it
Vets had the unfortunate task of putting down 26 of the swans on the Thames after six were pulled from the river earlier in January...
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

ADHD drug may protect against Alzheimer's neurodegeneration

ADHD drug may protect against Alzheimer's neurodegeneration | Virology News | Scoop.it
A repurposed ADHD medication that boosts norepinephrine levels could stall neurodegeneration in people with early signs of Alzheimer’s disease.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Doomscrolling is a unique behavior, not just a trendy name

Doomscrolling is a unique behavior, not just a trendy name | Virology News | Scoop.it
"Doomscrolling" isn't just a new name for an existing behavior, researchers say. It's a unique behavior all its own, according to a new study.
No comment yet.
Scooped by Ed Rybicki
Scoop.it!

Which countries offer the best (and worst) end-of-life care?

Which countries offer the best (and worst) end-of-life care? | Virology News | Scoop.it
"Most people in the world die badly—many through no treatment at all and many through excessive often futile treatment that increases suffering."...
No comment yet.