Plant and Seed Biology
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Rescooped by Loïc Lepiniec from Genetic Engineering - GEG Tech top picks
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Applying CRISPR/Cas for genome engineering in plants: the best is yet to come

Applying CRISPR/Cas for genome engineering in plants: the best is yet to come | Plant and Seed Biology | Scoop.it

Via BigField GEG Tech
BigField GEG Tech's curator insight, December 5, 2016 9:54 AM

Less than 5 years ago the CRISPR/Cas nuclease was first introduced into eukaryotes, shortly becoming the most efficient and widely used tool for genome engineering. For plants, efforts were centred on obtaining heritable changes in most transformable crop species by inducing mutations into open reading frames of interest, via non-homologous end joining.

Rescooped by Loïc Lepiniec from Genetic Engineering - GEG Tech top picks
Scoop.it!

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

Nucleosomes impede Cas9 access to DNA in vivo and in vitro | Plant and Seed Biology | Scoop.it

Via BigField GEG Tech
BigField GEG Tech's curator insight, April 5, 2016 10:05 AM

Our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 activity is incomplet. To address this gap, the scientists analyzed large-scale genetic screens performed in human cell lines using either nuclease-active or nuclease-dead Cas9 (dCas9). They observed that highly active sgRNAs for Cas9 and dCas9 were found almost exclusively in regions of low nucleosome occupancy. In vitroexperiments demonstrated that nucleosomes in fact directly impede Cas9 binding and cleavage, while chromatin remodeling can restore Cas9 access. These results reveal a critical role of eukaryotic chromatin in dictating the targeting specificity of this transplanted bacterial enzyme, and provide rules for selecting Cas9 target sites distinct from and complementary to those based on sequence properties.

Rescooped by Loïc Lepiniec from Genetic Engineering - GEG Tech top picks
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Second Chinese team reports gene editing in human embryos

Second Chinese team reports gene editing in human embryos | Plant and Seed Biology | Scoop.it
Study used CRISPR technology to introduce HIV-resistance mutation into embryos.

Via BigField GEG Tech
BigField GEG Tech's curator insight, April 12, 2016 9:18 AM

Researchers in China have reported editing the genes of human embryos to try to make them resistant to HIV infection. Their paper which used CRISPR-editing tools in non-viable embryos that were destroyed after three days is only the second published claim of gene editing in human embryos.

Rescooped by Loïc Lepiniec from Genetic Engineering - GEG Tech top picks
Scoop.it!

Genetic screens: CombiGEM-CRISPR: a creative combination - Nature Reviews Genetics 

Genetic screens: CombiGEM-CRISPR: a creative combination - Nature Reviews Genetics  | Plant and Seed Biology | Scoop.it

Via BigField GEG Tech
Loïc Lepiniecs insight:

Researchers have generated a platform that uses combinatorial genetics en masse (CombiGEM) and the CRISPR–Cas9 system for the rapid assembly of barcoded combinatorial genetic libraries that can be tracked with high-throughput sequencing.

BigField GEG Tech's curator insight, March 2, 2016 7:55 AM

Researchers have generated a platform that uses combinatorial genetics en masse (CombiGEM) and the CRISPR–Cas9 system for the rapid assembly of barcoded combinatorial genetic libraries that can be tracked with high-throughput sequencing.