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Institute for Bioengineering and Biosciences
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Intracoronary Delivery of Human Mesenchymal/Stromal Stem Cells

Intracoronary Delivery of Human Mesenchymal/Stromal Stem Cells | iBB | Scoop.it

Mesenchymal stem/stromal cells have unique properties favorable to their use in clinical practice and have been studied for cardiac repair. However, these cells are larger than coronary microvessels and there is controversy about the risk of embolization and microinfarctions, which could jeopardize the safety and efficacy of intracoronary route for their delivery. The index of microcirculatory resistance (IMR) is an invasive method for quantitatively assessing the coronary microcirculation status. In a recent publication in PlosOne, BERG-iBB researchers in collaboration with colleagues from the Department of Cardiology at Hospital de Santa Marta and from the Faculty of Veterinary Medicine at University of Lisbon have examined heart microcirculation in a swine model after intracoronary injection of mesenchymal stem/stromal cells with the index of microcirculatory resistance. Overall, the study provides definitive evidence of microcirculatory disruption upon intracoronary administration of mesenchymal stem/stromal cells, in a large animal model closely resembling human cardiac physiology, function and anatomy.

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A Microcarrier-based Stirred Culture System for the Scalable expansion of Human Mesenchymal Stem/Stromal Cells

A Microcarrier-based Stirred Culture System for the Scalable expansion of Human Mesenchymal Stem/Stromal Cells | iBB | Scoop.it

The therapeutic use of human mesenchymal stem/stromal cells (MSC) for a wide range of diseases is at the front line of stem cell-based cellular therapies. Through a bioprocess engineering approach, researchers at BERG-iBB led by Cláudia Lobato Silva and Joaquim Cabral, were able to maximize the production of MSC from different human sources – bone marrow and adipose tissue - in a microcarrier-based stirred culture system. The advances, reported in a recent paper on Biotechnology Journal, clearly improve the scalability and cost-effectiveness of MSC manufacturing, while facilitating the translation to a Good Manufacturing Practices (GMP)-compliant bioprocess.

 

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Seminar on "Stem Cell Niches" by Professor Graça Almeida-Porada

Seminar on "Stem Cell Niches" by Professor Graça Almeida-Porada | iBB | Scoop.it

Professor Graca Almeida-Porada, a renowed specialist in the area of Regenerative Medicine will be giving a talk entitled "Cell Therapies: Manipulating Stem Cell Niches" next Thursday the 18th June, at 11h00m, in Room 1.38, IST (Tagus Park). Prof. Almeida-Porada is currently affiliated with the Institute for Regenerative Medicine at Wake Forest Baptist Medical Center. She will be hosted by Prof. Cláudia Lobato Silva from BERG-iBB with whom she has been collaborating over the past years.

 

 

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Tiago Dias Selected to the Malcolm Lilly Award

Tiago Dias Selected to the Malcolm Lilly Award | iBB | Scoop.it

Tiago Dias, Ph. D. student in Biotechnology, has been shortlisted to present an oral lecture in the Malcolm Lilly Award session at the ESBES symposium, Lille 7-10th September. This prestigeous award is offered by the ESBES to a promising young scientist  working in the field of biochemical engineering  to honor Professor Malcolm Lilly, the founding father of Biochemical Engineering, Tiago, one of the 4 candidates selected, will have an opportunity to present the work he has developed at BERG under the supervison of Joaquim Cabral and Margarida Diogo with a communication entitled "Integrated Platform for Derivation and Enrichment of Human Pluripotent Stem Cell-Derived Cardiomyocytes: Towards a Clinical Application".

 

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BERG Researchers Develop Bioreactor System Mesenchymal Stem Cell Expansion

BERG Researchers Develop Bioreactor System Mesenchymal Stem Cell Expansion | iBB | Scoop.it

"In a recent paper published on Biotechnology and Bioengineering, researchers working on the Stem Cell Bioengineering and Regenerative Medicine area of BERG, in collaboration with Louisiana State University and Life Technology Corporation, describe the successfull establishment of a xenogeneic-free microcarrier-based culture system for bone marrow mesenchymal stem/stromal cells (MSC) and adipose tissue-derived stem/stromal cell (ASC) cultivation. The process, which was developed using a 1L-scale controlled stirred-tank bioreactor, is aimed at generating the large amounts of cells required for MSC-based clinical trials. Click on title to learn more."

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Production of Platelets and Their Progenitors From Umbilical Cord Blood

Production of  Platelets and Their Progenitors From Umbilical Cord Blood | iBB | Scoop.it

Platelet transfusion can be a life-saving procedure in different medical settings, but shelf-life of this plod product is only 5 days. The efficient ex vivobiomanufacturing of platelets would make it possible toovercome the shortages of donated platelets. The optimized protocol described by BERG researchers in a recent paper published in the journal Cythotherapy allows the efficient production of platelets and their progenitors from blood stem/progenitor cells by mimicking the bone marrow niche through a co-culture system with human mesenchymal stromal cells (MSC), in combination with different biological factors to prompt differentiation toward functional platelets. Click on title to learn more.

 

Photo details: SEM image from blood shwoing small disc-shaped platelets among other red and white blood cells. Bruce Wetzel, NCI, 1982, public domain. 

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Seminar on Bioengineered Organs by Professor Pedro Baptista

Seminar on Bioengineered Organs by Professor Pedro Baptista | iBB | Scoop.it

Professor Pedro Baptista, a specialist in the area of  of Regenerative Medicine will be giving a talk entitled "How to make a liver and other organs for Dummies" Friday the 19th June, at 11h00m, in Room QA1.2, IST (Alameda). Pedro Baptista is currently a Group Leader at the Aragon Health Sciences Institute (IACS) in Zaragoza, Spain and the founder of the Organ Bioengineering and Regenerative Medicine Laboratory at the Aragon Biomedical Research Institute, Zaragoza, Spain. The focus of his work is on intestinal organs bioengineering and cellular therapies directed to liver, pancreas and kidney regeneration. He will be hosted by Prof. Cláudia Lobato Silva from BERG-iBB.

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Seminar on Human Hematopoietic Stem Cells by Dr Fernando Anjos-Afonso

Seminar on Human Hematopoietic Stem Cells by Dr Fernando Anjos-Afonso | iBB | Scoop.it

Dr. Fernando Anjos-Afonso of the European Cancer Stem Cell Research Institute at Cardiff School of Biosciences, will be giving a talk entitled "Notching up Human Hematopoietic Stem Cells to control their cell fate decisions and expansion" Friday the  8th May, at 11h00m, in Room 1.38, IST (TagusPark). Dr. Anjos-Afonso was awarded the Jane Hodge Research Fellowship at the European Cancer Stem Cell Research Institute in 2015, to investigate different cell-to-cell communication signalling pathways that control human haematopoietic stem cell activity and to determine how these pathways are exploited or avoided by certain leukaemias.

 

Photo: A colony of embryonic stem cells (centre). Ryddragyn 2006, Public domain.  https://commons.wikimedia.org/wiki/File:Humanstemcell.JPG

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3D Microarray Platform for Toxicology Assays in Neural Development

3D Microarray Platform for Toxicology Assays in Neural Development | iBB | Scoop.it

In collaboration with Rensselaer Polytechnic Institute (RPI/USA), BERG researchers at the Stem Cell Bioengineering and Regenerative Medicine Laboratory (SCBL) have recently developed a 3D microarray platform to perform high-throughput studies of human Neural Stem Cell (hNSC) Differentiation and Toxicology. By using this platform it is possible to screen for the differential toxicity of small molecules to hNSCs which may help to predict, in vitro, which compounds pose an increased threat to neural development and should therefore be prioritized for further screening and evaluation. The work was published in “Stem Cell Research” journal. Click on title to learn more,

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