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Institute for Bioengineering and Biosciences
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Mobile‐Phase Modulators as Salt Tolerance Enhancers in Phenylboronate Chromatography

Mobile‐Phase Modulators as Salt Tolerance Enhancers in Phenylboronate Chromatography | iBB | Scoop.it

The mechanisms underlying the adsorption of monoclonal antibodies (mAbs) towards phenylboronate (PB) ligands have been elucidated by Flow Microcalorimetry. The knowledge acquired BERG-iBB researchers, in collaboration with Cristina Dias-Cabral's group from CICS-UBI, suggest how to increase the salt tolerance of the bond between ligand and biomolecule by manipulating the environmental conditions.It was demonstrated that such feature can be accomplished either by using mobile phase modulators that promote the specific cis-diol interactions between PB ligands and mAbs or by diminishing the non-specific interactions involved in the adsorptive process. The research team believes that these findings could contribute towards the effective integration of boronate chromatography in the current mAb downstream processing platform. The paper was published in Biotechnology Journal.

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Flow Microcalorimetry Analysis of the Adsorption of mAbs in Phenylboronate Chromatography

Flow Microcalorimetry Analysis of the Adsorption of mAbs in Phenylboronate Chromatography | iBB | Scoop.it

The feasibility of applying Flow Microcalorimetry to evaluate the complex phenomena involved in the adsorption of monoclonal antibodies to phenylboronate ligands has been demonstrated by BERG-iBB researchers led by Ana Azevedo, in collaboration with Ana Cristina Dias-Cabral from CICS-UBI. The thermodynamic studies evidence the role of affinity and non-specific effects that contribute to the complex, phenylboronate-mediated multimodal adsorption process. This deeper understanding, combined with the economic advantages associated with the employment of the PBA ligand, may lead industry to adopt such a chromatographic process as a reliable alternative to protein A-based capture steps in the current downstream processing of mAbs. The work is part of Sara Rosa’s PhD thesis in Biotechnology and Biosciences. The paper was published on J. Chromatography A.

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Sara Rosa Attends Bayer PhD Course 2016

Sara Rosa Attends Bayer PhD Course 2016 | iBB | Scoop.it

Sara Rosa, a PhD student from BERG-iBB attended the Bayer PhD course 2016 in Germany from the 7th to the 10th March. During this week, PhD students with  Mechanical, Electronic Engineering and Biotechnology backgrounds  and from diverse parts of Europe, EUA and India had the opportunity to know more about Bayer’s modus operandi, mission and values. The students worked as teams and discussed their PhD projects, visited the Leverkusen and Wüppertal site plants, met people working in the various company segments from Technology Development, Process Control Technologies to Supply Chain and got to know a bit of the story and the beauties of the main city Köln. About Bayer: Bayer is a global enterprise with core competencies in the Life Science Fields of health care and agriculture. Click on title to know more.

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Sara Rosa to Defend PhD Thesis in Biotechnology and Biosciences

Sara Rosa to Defend PhD Thesis in Biotechnology and Biosciences | iBB | Scoop.it

Sara Lourenço Rosa will be defending her PhD thesis in Biotechnology and Biosciencesat Instituto Superior Técnico, monday the 9th september 2019 (14:30 H, room PA-3). During the last years, and under the supervision of Ana Azevedo from BERG-iBB and Cláudia Lobato-Silva from SCERG-iBB, Sara explored phenyl boronate chromatography as an alternative technique to purify biomolecules such as antibodies. The title of her thesis is "A phenylboronate chromatography based approach as an effective alternative for downstream processing of biomolecules".

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iBB-BERG Represented at ISPPP 2016

iBB-BERG Represented at ISPPP 2016 | iBB | Scoop.it
iBB was recently represented at the International Symposium ISPPP 2016 by researchers from BERG's Bioseparation Engineering Lab, who presented 3 oral communications (Raquel Aires-Barrros, Ana Azevedo and Emanuel Capela) and 2 poster communications (Sara Rosa and Inês Pinto).
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